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1992 Fiscal Year Final Research Report Summary

New vasoactive peptides and their functions

Research Project

Project/Area Number 03670136
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionNational Cardiovascular Center Research Institute (NCVCRI)

Principal Investigator

MINAMINO Naoto  NCVCRI, Pharmacology, Head, 薬理部, 室長 (50124839)

Co-Investigator(Kenkyū-buntansha) MATSUO Hisayuki  NCVCRI, General Director, 所長 (50028685)
Project Period (FY) 1991 – 1992
KeywordsVasoactive peptide / Vasocular smooth muscle cell / Vascular endothelial cells / Natriuretic peptide / Second messenger / Growth inhibition / C-type natriuretic peptide / Natriuretic peptide receptor
Research Abstract

(1) Development of survey methods for new vasoactive peptides : In addition to our previous methods using contractile or relaxant activity on several smooth muscle preparations, We developed a new method measuring second messengers in the cell, such as cAMP and cGMP, in cultured vascular smooth muscle cells and endothelial cells.
(2) production of CNP in THP-1 cell line : We detected strong chick rectum relaxant activity in phorbol-ester (TPA) supplemented culture medium of monocytic leukemia cell line, THP-1. From the culture medium, we purified a peptide exhibiting rectum relaxant activity. The peptide was determined to be CNP-29 by structural analyses.
Since vascular smooth muscle cells (VSMC) express CNP-specific GC-B receptor, CNP derived from monocytes (or macrophages) may regulate VSMC functions.
(3) C-type natriuretic peptide (CNP) : CNP has been recognized as a neuropeptide functioning in the central nervous system. By using the PCR method and highly sensitive RIA method, we found CNP and its mRNA in the peripheral tissue, including aorta, kidney, lung, gastrointestinal tract. These results suggest that CNP may be a local regulator functioning through paracrine mode.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Y.OHYAMA,et.al.: "Cloning and characterization of two forms of C-type natriuretic peptide receptor in rat brain." Biochem.Biophys.Res.Commun.183. 743-749 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.FURUYA,et.al.: "Structural reqiurements of C-type natriuretic peptide for elevation of cyclic GMP in cultured vascular smooth muscle cells." Biochem.Biophys.Res.Commun.183. 964-969 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.TATEYAMA,et.al.: "Concentrations and molecular forms of human brain natriuretic peptide in plasma." Biochem.Biophys.Res.Commun.185. 760-767 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.TANAKA,et.al.: "Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells." Proc.Natl.Acad.USA. 89. 8928-8932 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.OHYAMA,et.al.: "Stable expression of natriuretic peptide receptors:effects of HS-142-1,a non-peptide ANP antagonist." Biochem.Biophys.Res.Commun.189. 336-342 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.ISHIZAKA,et.al.: "Isolation and identification of C-type natriuretic peptide in human monocytic cell line,THP-1." Biochem.Biophys.Res.Commun.189. 697-704 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 寒川 賢治他: "血管の分子生物学3.血管作動性物質とリセプター3.ナトリウム利尿ペプチドファミリー(ANP・BNP・CNP)その生化学" 中外医学社(高久 史磨監修永井 良三,平井 久丸編), 19 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] 児島 将康他: "循環調節ペプチドと関連疾患ナトリウム利尿ペプチドファミリー(ANP.BNP.CNP)の構造と発現" 羊土社(矢崎 義雄編), 12 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] K.J.Koller, D.G.Lowe, G.L.Bennett, N.Minamino, K.Kangawa, H.Matsuo and D.V.Goeddel: "Selective activation of the B natriuretic peptide receptor by C-type natriuretic peptide (CNP)" Science. 252. 120-123 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M.Aburaya, E.Suzuki, N.Minamino, K.Kangawa, K.Tanaka and H.Matsuo: "Concentration and molecular forms of brain natriuretic peptide in rat plasma and spinal cord" Biochem. Biophys. Res. Commun. 177. 40-47 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Kozawa, J.Hino, N.Minamino, K.Kangawa and H.Matsuo: "Isolation of four novel tachykinins from frog (Rana catesbeiana) brain and intestine" Biochem. Biophys. Res. Commun. 177. 588-595 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M.Furuya, M.Yoshida, Y.Hayashi, N.Ohnuma, N.Minamino, K.Kangawa and H.Matsuo: "C-type natriuretic peptide is a growth inhibitor of rat vascular smooth muscle cells" Biochem. Biophys. Res. Commun. 177. 927-931 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Minamino, Y.Makino, H.Tateyama, K.Kangawa and H.Matsuo: "Characterization of immunoreactive human C-type natriuretic peptide in brain and heart" Biochem. Biophys. Res. Commun. 179. 535-542 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] E.Suzuki, Y.Hirata, H.Matsuoka, T.Sugimoto, H.Hayakawa, T.Sugimoto, K.Kangawa, N.Minamino and H.Matsuo: "Characterization of atrial natriuretic peptide in urine from rats treated-with a neutral endopeptidase inhibitor" Biochem. Biophys. Res. Commun. 182. 1270-1276 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ohyama, K.Miyamoto, Y.Saito, N.Minamino, K.Kangawa and H.Matsuo: "Cloning and characterization of two forms of c-type natriuretic peptide receptor in rat brain" Biochem. Biophys. Res. Commun. 183. 743-749 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Tateyama, J.Hino, N.Minamino, K.Kangawa, T.Minamino, K.Sakai, T.Ogihara and H.Matsuo: "Concentrations and molecular forms of human brain natriuretic peptide in plasma" Biochem. Biophys. Rse. Commun. 185. 760-767 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Tanaka, K.Miyamato, N.minamino, M.Takeda, B.Sato, H.Matsuo and K.Matsumoto: "Cloning and characterization of an androgen-induced growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells" Proc. Natl. Acad. Sci. USA. 89. 8928-8932 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ohyama, K.Miyamoto, Y.Morishita, Y.Matsuda, Y.Saito, N.Minamino, K.Kangawa and H.Matsuo: "Stable expression of natriuretic peptide receptors : Effects of HS-142-1, a non-peptide ANP antagonist" Biochem. Biophys. Res. Commun. 189. 336-342 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ishizaka, K.Kangawa, N.Minamino, K.Ishii, S.Takano, T.Eto and H.Matsuo: "Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1" Biochem. Biophys. Res. Commun. 189. 697-704 (1992)

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Published: 1994-03-24  

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