1992 Fiscal Year Final Research Report Summary
Mechanism of T cell growth-signal transduction and its abnormality
| Project/Area Number |
03670172
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
NAMBA Yuziro Inst. for Virus Res., Kyoto Univ. Associate Prof., ウイルス研究所, 助教授 (50027322)
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| Project Period (FY) |
1991 – 1992
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| Keywords | protein phosphorylation / T lymphocyte / 1-plastin / IL 2 / actin-binding protein / calcium-binding protein / monoclonal antibody / microfilament |
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| Research Abstract |
65-kilodalton protein has been characterized as an interleukin 2-stimulated phosphoprotein in human T cells. We determined the complete primary structure of p65 based on the cDNA isolated from a human T lymphocyte (KUT-2) cDNA library. Analysis of p65 sequence indicated that p65 is multidomain molecule involving at least three intriguing domains; two putative calcium-binding sited along the N- terminal 80 amino acid residues; a putative calmodulin-binding site following the calcium binding region; and two tandem repeats of putative actin-binding domains in its middle and C-terminal parts, each containing approximately 240 residues. This domain structure closely resembles a chicken intestinal microvilli protein, fimbrin, that bundles actin filaments. It was observed that unphosphorylated L-plastin isolated from human T cells bundles F-actin just as fimbrin does. L-plastin acted on T cell beta-actin, but hardly acted on muscle alpha-actin or chicken gizzard gamma-actin, whereas fimbrin bundles alpha-actin. Unlike fimbrin, L-plastin's actin-bundliung action was strictly calcium-dependent: the bundles were formed at pCa 7, but not at pCa 6. Under suitable conditions, approximately one molecule of L-plastin bound to 8 molecules of actin monomer in the actin filament.
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[Publications] Kondo,S.,Miyatake,S.,Oda,Y.,Kikuchi,H.,〓 Namba,Y.,: "Analysis of the close relationship between human astrocytoma-specific antigens detected by murine monoclonal antibodies and c-kit proto-oncogene products." Biochem.Biophys.Res.Comm.,. 182. 474-480 (1992)
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「研究成果報告書概要(和文)」より
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