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1992 Fiscal Year Final Research Report Summary

Suppression of tumor phenotypes of human colon carcinoma cells by introduction of normal chromosomes

Research Project

Project/Area Number 03670186
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionThe Tokyo Metropolitan Institute of Medical Science

Principal Investigator

TANAKA Kiyoko  Dept. Biochem. Researcher, 研究員 (40124474)

Project Period (FY) 1991 – 1992
KeywordsHuman colon cancer / chromosome introduction / Tumor suppressor gene / 微小核融合法 / 癌形質抑制
Research Abstract

Development of colon carcinomas can be associated with allelic deletions on several chromosomes, including 5q and 17p. The APC gene on 5q and the p53 gene on 17q have been identified as potential tumor suppressor genes, whose suppression contributes to colon carcinogenesis. To investigate the role of genes in these deleted regions, we have introduced different normal human chromosomes into a colon carcinoma cells through microcell hybridization. The introduction of a normal chromosome 17 causes a decrease in the malignancy of colon carcinoma cells suppressing certain malignant phenotypes, such as morphology, growth in soft agar, although not all tumor phenotypes are suppressed. The hybrid cells with chromosome 17 were tumorigenic, but the tumor growth rate of these clones were consistently slower than that of parental colon carcinoma cells. Furthermore, the introduction of normal chromosome 5 into hybrid cells with chromosome 17 caused suppression of the growth of carcinoma cells. Introduction of different normal chromosomes into colon carcinoma cells brought a decrease in the malignancy.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] K.TANAKA et al.: "Suppression of tumorigenicety in human colon carcinoma cells by introduction of normal chromosome 1p36 region." Onocogene.(1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.SEKI,K.TANAKA et al.: "Loss of allele of the APC gene in an adrenocortical carcinoma from a patient in a family of adenomatous polypossis." Human Gent.89. 298-300 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.YANOSHITA,K.YANAKA et al.: "Genetic changes in both alleles of p53gene involved in the conversion of colorectal adenomas into early carinomas." Cancer Res.52. 3965-3971 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.TANAKA et al.: "Suppresseon of tumorigenicety in human colon carcinoma cells by introduction of normal chromosome 5 or 18." Nature. 349. 340-342 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中 貴代子 他: "染色体移入による癌形質の抑制" 実験医学. 9. 59-62 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宮木 美知子,田中 貴代子 他: "多段階発癌過程におけるp53遺伝子異常の意義" 実験医学. 10. 964-968 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanaka et al: "Suppression of tumorigenicity in human colon carcinoma cells by introduction of normal chromosome 1p36 region" Onocogene. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Seki, K.Tanaka et al: "Loss of allele of the APC gene in an adrenocortical carcinoma from patient in a family of adenomatous polypossis" Human Gent. 89. 298-300 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Yanoshita, K.Tanaka et al: "Genetic changes in both alleles of p53 gene involved in the conversion of colorectal adenomas into early carcinomas" Cancer Res. 52. 3965-3971 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka et al: "Suppression of tumorigenicity in human colon carcinoma cells by introduction of normal chromosome 5 or 18" Nature. 349. 340-342 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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