1992 Fiscal Year Final Research Report Summary
Immune mechanisms in parasite-snail interactions
| Project/Area Number |
03670199
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Kagoshima University |
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Principal Investigator |
NODA Shinichi Kagoshima University Faculty of Medicine Associate Professor, 医学部, 助教授 (60112439)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIKAWA Ryuichi Kyoto Prefectural University of Medicine Research Associate (only 1991), 助手 (80145466)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Invertebrate / Echinostoma paraensei / Biomphalaria glabrata / Defense system / Secretory products / Hemocyte / Hematopoietic organ / Chemotaxis |
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| Research Abstract |
Effects of excretory-secretory (E-S) products of Echinostoma paraensei larvae on hematopoietic organ and hemocyte motility of Biomphalaria glabrata were studied.
Eggs of E. paraensei were suspended in culture medium, and hatched larvae were cultured to collect E-S products. M-line B. glabrata snails were injected with E-S products, and the size of the HO was ascertained in histological sections. The size of HO in snails injected with E-S products from sporocysts was significantly larger than that in snails injected with culture medium. E-S products of sporocysts were fractionated using ultrafiltration membranes, polyacrylamide gel electrophoresis, and electrophoretic elution. Examination of fractionated E-S products of sporocysts revealed that specific components of E-S products were responsible for HO-stimulating activity.
Both whole (unfractionated) and fractionated E-S products were tested in chemotaxis chambers. E-S products of sporocysts were fractionated using ultrafiltration membranes. E-S products of sporocysts significantly inhibited the random motility of hemocytes from M-line B. glabrata.
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