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1992 Fiscal Year Final Research Report Summary

studies on the mechanism by which nasal influenza vaccine enhances the efficacy of protection

Research Project

Project/Area Number 03670241
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionNational Institute of Health

Principal Investigator

TAMURA Shin-ichi  N.I.H. chief, 感染病理部, 室長 (20100084)

Co-Investigator(Kenkyū-buntansha) KURATA Takeshi  N.I.H. Director, 感染病理部, 部長 (50012779)
Project Period (FY) 1991 – 1992
Keywordsinfluenza vaccine / cholera toxin B subunit / hemagglutinin / uncleoprotein / CTL / nasal vaccination / 経鼻接種ワクチン
Research Abstract

In our previous studies, We demonstrated that intranasal inoculation of influenza inactivated vaccine together with cholera toxin B subunit(CTB)into mice can provide cross-protection against variant virus infection in parallel with the induction of cross-reacting IgA antibodies (Ab) in the respiratory tract. The inactivated vaccine contained seven protein components of influenza virus. Among them, surface glycoprotein, hemagglutinin(HA), which varies slightly within the same subtype and largely within the same type, is known to be a major protein involved in the induction of protective antibodies. In addition, core protein, nucleoprotein(NP), which is cross-reactive in the same type, is known to be another major component involved in the recovery from influenza. The present study was designed to elucidate the role of HA and NP in the defense mechanism against influenza in the mice immunized intranasally together with CTB. This would lead to the development of the effective nasal influenza component vaccine. The results show that intranasal inoculation of PR8 HA molecules together with CTB into mice conferred protection against not only homologous virus infection but also heterologous virus infection. Moreover, the respiratory tract anti-HA IgA Ab from mice immunized with CTB-combined HA molecules mediated directly the cross-protection when transferred passively into the respiratory tract. Thus,the HA is one of the essential vaccine components in the blockade of infection in the nasally-vaccinated mice. On the other hand, intranasal inoculation of PR8 NP molecules together with CTB induced memory cells involved in the accelerated induction of CTL responses. Moreover, the accelerated elimination of virus-infected cells by CTL in the respiratory tract resulted in the facilitation of the recovery from influenza. Consequently, the NP seems to be also a useful vaccine component under the absence of local Ab.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] TAMURA,S.-I.,et al.: "Cross-protection against influenza A virus infection by passively transferred respiratory tract IgA antibodies to different hemagglutinin molecules." Eur.J.Immunol.21. 1337-1344 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIRABAYASHI,Y.,et al.: "H-2-unrestricted adjuvant effect of cholera toxin B subunit on murine antibody responses to influenza virus hemagglutinin." Immunology. 72. 329-335 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAMURA,S.-I.,et al.: "Superior cross-protective effect of nasal vaccination to subcutaneous inoculation with influenza hemagglutinin vaccine." Eur.J.Immunol.22. 477-481 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] HIRABAYASHI,Y.,et al.: "Inovolvement of antigen-presenting cells in the enhance-ment of the in vitro antibody responses by cholera toxin B subunit." Immunology. 75. 493-498 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gizurarson,S.,et al.: "Stimulation of the transepithelial flux of influenza HA vaccine by cholera toxin B subunit." Vaccine. 10. 101-106 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TAMURA,S.-I.,et al.: "Cross-protection against influenza virus infection afforded by trivalent inactivated vaccines inoculated intranasally with cholera toxin B subunit." J.Immunol.149. 981-988 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura,S.-I., et al.: "Cross-protection against influenza A virus infection by passively transferred respiratory tract IgA antibodies to different hemagglutinin molecules." Eur.J. Immunol.21. 1337-1344 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirabayashi,Y., et al.: "H-2-unrestricted adjuvant effect of cholera toxin B subunit on murine antibody responses to influenza virus hemagglutinin" Immunology. 72. 329-335 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura,S.-I., et al.: "Superior cross- protective effect of nasal vaccination to subcutaneous inoculation with influenza hemagglutinin vaccine" Eur. J. Immunol.22. 477-481 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirabayashi,Y., et al.: "Involvement of antigen-presenting cells in the enhancement of the in vitro antibody responses by cholera toxin B subunit" Immunology. 75. 493-498 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gizurarson,S., et al.: "Stimulation of the transepithelial flux of influenza HA vaccine by cholera toxin B subunit" Vaccine. 10. 101-106 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura,S.-I., et al.: "Cross-protection against influenza virus infection afforded by trivalent inactivated vaccines inoculated intranasally with cholera toxin B subunit" J. Immunol.149. 981-988 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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