1992 Fiscal Year Final Research Report Summary
A study on the cell interaction between stromal cells and lymphocytes
| Project/Area Number |
03670246
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KINA Tatsuo Chest Disease Research Institute, Kyoto University Associate Professor, 胸部疾患研究所, 助教授 (30127071)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Stromal cell / Adhesion molecule / Cell interaction / Lymphocytes / VCAM-1 / VLA-4 |
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| Research Abstract |
Function of cell surface 107kD glycoprotein(gp107) of stromal cells in lymphocyte development was investigated We have established two monoclonal antibodies (HANA-2 and HANA-5) against 107kD glycoprotein(s),and studied the expression and function of gp107 in hematopoietic system. In normal hematopoietic system, HANA-2 reacts only with fibroblastoid cells in the reticuloendothelia system, but not with lymphoid, myeloid and epithelial cells. On the other hand, HAHAN-5 reacts with activated lymphocytes and myeloid cells as well as established stromal cells. In the in vitro hematopoietic system, although HANA-5 strongly inhibited the development of both lymphoid and myeloid cells, HANA-2 inhibited only lymphoid development. HANA-2 but not HANA-5 could block the binding of lymphocytes with stromal cells, suggesting that HANA-2 reacts with an adhesion molecule on the stromal cells. By isolating cDNA clones for HANA-2 antigen, we identified it as an moue homologue of human vascular cell adhesion molecule, VCAM-1. This was further confirmed by the findings that antibodies against mouse VLA-4 strongly blocked lymphocye adhesion to stromal cells. HANA-5 has no blocking effects on cell adhesion and seems to be an activated antigen of both hamatopoietic and stromal cells, These results suggest an important role of VLA-4/VCAM-1 interaction in the development of lymphoid cells and that 107kD poteins on the stromal cell surface consist of at least two different molecules, one of which is VCAM-1.
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[Publications] Kina, T., Sen Majumdar, A., Heimfeld, S., Kaneshima, H., Holzmann, B., Katsura, Y., and Weissman, I. L.: "Identification of a 107-kD glycoprotein that mediates adhesion between stromal cells and hematolymphoid cells." J. Exp. Med.173. 373-381 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Ogawa, M., Matsuzaki, Y., Nishikiwa, S., Hayashi,S-I., Kunisada, T., Sudo, T., Kina, T., Nakauchi, H. and Nishikawa S-I.: "Expression and function of c-kit in hemopoietic progenitor cells." J. Exp. Med.174. 63-71 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Ikuta, K., Kina, T., MacNeil, I., Uchida, N., Peault, B., Chien, Y. and Weissman, I. L.: "Development of gammadelta T-cell subsets from fetal hematopoietic stem cells." Ann. N. Y. Acad. Sci.651. 21-32 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Ogawa, M., Nishikawa, S., Yoshinaga, K., Hayashi, S-I., Kunisada, T., Nakao, J., Kina, T., Sudo, T., Kodama, H., and Nishikawa, S-I.: "Expression and function of c-Kit in fetal hemopoietic progenitor cells: transition from the early c-kit-independent to the late c-kit-dependent wave of hemopoiesisin the murin embryo." Development.117. (1993)
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「研究成果報告書概要(欧文)」より
