1993 Fiscal Year Final Research Report Summary
血管病変の発症と進行における内皮細胞補体産生の役割
| Project/Area Number |
03670276
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hygiene
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| Research Institution | Kawasaki Med. School |
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Principal Investigator |
UEKI Ayako Kawasaki Med. School, Hygiene, Professor, 医学部, 教授 (10069031)
UEKI Ayako Kawasaki Med. School, Hygiene, Professor (10069031)
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| Co-Investigator(Kenkyū-buntansha) |
UEKI Ayako Kawasaki Med. School, Hygiene, Professor (10069031)
UEKI Ayako Kawasaki Med. School, Hygiene, Professor (10069031)
UEKI Ayako Kawasaki Med. School, Hygiene, Professor (10069031)
UEKI Ayako Kawasaki Med. School, Hygiene, Professor (10069031)
|
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| Project Period (FY) |
1991 – 1993
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| Keywords | endothelial cells / complement / cytokines |
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| Research Abstract |
Human umbilical cord vein endothelial cells (HUVEC) are known to synthesize and secrete complement components, and we reported previously the synthesis of Complement component 3 (C3). When we assayd the amount of C3 and factor B (Bf) with ELISA in culture supernatant of HUVEC stimulated with several king of cytokines, TNF-alpha had the strongest effect on the synthesis of complement components, and TNF-beta, IL-1beta and IFN-gamma also demonstrated the stimulating effect. There results were in accoydance with the mRNA level in the cells examined with Nothern blotting method. Combination of TNF-alpha and IL-1beta or TNF-alpha and IFN-gamma had synergistic effects on the release of C3 or Bf respectivly.
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