• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

血管病変の発症と進行における内皮細胞補体産生の役割

Research Project

Project/Area Number 03670276
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionKawasaki Med. School

Principal Investigator

UEKI Ayako  Kawasaki Med. School, Hygiene, Professor, 医学部, 教授 (10069031)

UEKI Ayako  Kawasaki Med. School, Hygiene, Professor (10069031)

Co-Investigator(Kenkyū-buntansha) UEKI Ayako  Kawasaki Med. School, Hygiene, Professor (10069031)
UEKI Ayako  Kawasaki Med. School, Hygiene, Professor (10069031)
UEKI Ayako  Kawasaki Med. School, Hygiene, Professor (10069031)
UEKI Ayako  Kawasaki Med. School, Hygiene, Professor (10069031)
Project Period (FY) 1991 – 1993
Keywordsendothelial cells / complement / cytokines
Research Abstract

Human umbilical cord vein endothelial cells (HUVEC) are known to synthesize and secrete complement components, and we reported previously the synthesis of Complement component 3 (C3). When we assayd the amount of C3 and factor B (Bf) with ELISA in culture supernatant of HUVEC stimulated with several king of cytokines, TNF-alpha had the strongest effect on the synthesis of complement components, and TNF-beta, IL-1beta and IFN-gamma also demonstrated the stimulating effect. There results were in accoydance with the mRNA level in the cells examined with Nothern blotting method. Combination of TNF-alpha and IL-1beta or TNF-alpha and IFN-gamma had synergistic effects on the release of C3 or Bf respectivly.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Y.Kawakami,A.Ueki et al.: "Regulation of C3 and FB production by endothelial cells with TNF-α." Molecular Immunology. 30. 22 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Watanabe,A.Ueki et al.: "Human CD4^+CD45RA^+ T lymphocytes can be stimulated by crocidolite,anthophyllite and amosite asbestos in vitro." Int.J.Oncology. 2. 209-212 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Ueki et al.: "Synthesis of complement C3 by human umbilical cord vein endothelial cells in vitro." Abstracts of International Congress of Immunology.8. 174 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Ueki et al.: "Crocidolite asbestos suppress the differentiation of HL-60 cells induced by DMSO." Cancer letters. 62. 225-232 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tomita,A.Ueki et al.: "Combined action of paraquat and superoxide on the peroxidation of detergent-dispersed linolenic acid." Biochim.Biophys.Acta. 1128. 174-180 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 川上,植木 他.: "ヒト血管内皮細胞による補体産生へ及ぼすサイトカインの影響" 炎症. 12. 489-491 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Kawakami & A.Ueki: "Regulation of C3 and FB production by endothelial cells with TNF-alpha." Molecular Immunology. 30. 22 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Watanabe, M.Yamaguchi, Y.Kawakami, F.Hyodoh, H.Tsushima, G.Ohsawa & A.Ueki: "Human CD4^+CD45RA^+ T lymphocytes can be stimulated by crocidolite, anthophyllite and amosite asbestos in vitro" Int.J.Oncology. 2. 209-212 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Ueki, Y.Kawakami, Y.Watanabe, H.Sakaguchi, J.Kanda, H.Tsushima & F.Hyodoh: "Synthesis of complement C3 by human umbilical cord vein endothelial cells in vitro." Abstracts of International Congress of Immunology. 8. 174 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Ueki, H.Tsushima, F.Hyodoh, K.Kinugawa, M.Tomita, J.Kazahaya & R.Shirato: "Crocidolite asbestos suppress the differentiation of HL-60 cells induced by DMSO" Cancer letters. 62. 225-232 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kinugawa, A.Ueki, M.Yamaguchi, Y.Watanabe, Y.Kawakami, F.Hyodoh & H.Tsusima: "Activation of human CD4^+CD45RA^+ T cells by chrysotile asbestos in vitro" Cancer letters. 66. 99-106 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Tsushima, H.Mine, & A.Ueki: "Candida albicans produces a cystatin type-cysteine protease inhibitor" J.Bacteriology. 174. 4807-4810 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tomita, T.Okuyama & A.Ueki: "Combined action of paraquat and superoxide on the peroxidation of detergent-dispersed linolenic acid" Biochim.Biophys.Acta. 1128. 174-180 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi