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1992 Fiscal Year Final Research Report Summary

The analysis of immune response and cytotoxic mechanism with use of anti-ICAM-1 monoclonal antibodies

Research Project

Project/Area Number 03670330
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionSapporo Medical College

Principal Investigator

TSUJISAKI Masayuki  Sapporo Med. College, Dept. of Int. Med., Instructor, 医学部, 助手 (50217311)

Co-Investigator(Kenkyū-buntansha) YACHI Akira  Sapporo Med. College, Dept. of Int. Med., Prof., 医学部, 教授 (50045324)
Project Period (FY) 1991 – 1992
Keywordsadhesion modecule / ICAM-1 / circulating antigen / cytotoxic mechanism / metastasis and invasion of cancer cells
Research Abstract

A new monoclonal antibody (MoAb) HA58 (IgGl) was prepared, which recognizes the binding site on the intercellular adhesion molecule-1 (ICAM-1) antigen to the lymphocyte function-associated antigen-1 (LFA-1). The double-determinant immunoassay (DDIA) was established with use of MoAb HA58 and another anti-ICAM-1, MoAb CL207, to detect the soluble, shedding ICAM-1 antigen. The incidence of positivity for ICAM-1 antigen in malignant diseases was higher than that in benign diseases or in healthy controls. Immunostaining of cancerous tissue with MoAb HA58 showed that the ICAM-1 antigen was expressed not only on malignant cells but also on stromal cells (mainly fibroblasts) near cancer nests, and the stromal cells immediately adjacent to cancer nests, in comparison to those cells farther away from the nests, have a higher ICAM-1 expression. We speculate that tumor cells activate T cells to produce cytokines (e.g.IFN-gamma), resulting in strong expression of the ICAM-1 on surrounding fibroblasts, as well as on tumor cells. Shedding ICAM-1 antigen may also block the attachment of cytotoxic T cells and/or NK cells to cancer cells, since LFA-1 could be blocked with soluble ICAM-1. This could be one of the escape mechanisms of cancer cells from the immunosurveillance system of the host.

  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] K.Imai: "Increased circulating intercellular adhesion molecule-1 in adult T cell leukemia patients" Int.J.Hematology. 56. 239-240 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Shijubo: "Circulating ICAM-1 antigeu in seso of patients urth idiopathic pulmonery fibrosis" Clin.exp.Immunol.89. 58-62 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 谷内 昭: "CD54/ICAM-1分子" Medical Immunology. 24. 573-580 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻崎 正幸: "キラーリンパ球の動態" 臨床免疫. 25. 175-182 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻崎 正幸: "腫瘍(細胞接着因子の面から)" 日本臨床免疫学会誌. 15. 586-592 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 辻崎 正幸: "悪性腫瘍における接着分子" BIOmedica. 7. 37-41 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsujisaki,M., Imai,K., Hirata,H., Hanzawa,Y., Masuya,J., Nakano,T., Sugiyama,T., Matsui,M., Hinoda,Y. and Yachi,A.: "Detection of circulating intercellular adhesion molecule-1 antigen in maligant diseases." Clin. Exp. Immunol.85. 3-8 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shijubo,N., Imai,K., Aoki,S., Hirasawa,M., Sugawara,H., Koba,H., Tsujisaki,M., Sugiyama,T., Hinoda,Y., Yachi,A., Asakawa,M. and Suzuki,A.: "Circulating intercellular adhesion molecule-1 (ICAM-1) antigen in sera of patients with idiopathic pulmonary fibrosis." Clin. Exp. Immunol.89. 58-62 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imai,K., Nakano,T., Motoya,S., Araki,K., Tsujisaki,M., Hinoda,Y. and Yachi,A.: "Increased circulating ICAM-1 in adult T cell leukemia patients." Int. J. Hematology. 56. 239-240 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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