1992 Fiscal Year Final Research Report Summary
Expression and function of neural cell adhesion molecule (N-CAM) in small cell lung cancer
| Project/Area Number |
03670400
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
KAWASE Ichiro The Department of internal Medicine III, Osaka University Medical School, Assistant Professor, 医学部第3内科, 講師 (10161324)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Shinichi Osaka university Hospital, Post Doctoral Fellow, 医学部附属病院, 医員
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| Project Period (FY) |
1991 – 1992
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| Keywords | Small cell lung cancer / Neural cell adhesion molecule / N-CAM / Monoclonal antibody |
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| Research Abstract |
Murine lgG1 monoclonal antibody (mAb) designated ITK-2 was generated against a small cell lung cancer (SCLC) cell line. ITK-2 reacts with neuroendocrine tissues and tumors including SCLC, and recognize a 145kDa glycoprotein of SCLC cell membrane extracts, called neural cell adhesion molecule (N-CAM). This result was correlated with Northern blotting where a single 6.2 kb mRNA was detected in SCLC cell lines. To determine cDNA sequence encoding the N-CAM isoform, several cDNA clones were isolated from OS2-R, a SCLC cell line established in our laboratory. Based on the analysis of the full-length cDNA obtained from two clones, the sequence of this 145 kDa isoform was shown to be essentially identical to that of the 140 kDa N- CAM isoform of neuroblastoma except two differences which include the lack of 30-bp insert termed the VASE exon and a single base pair change at position 1620 without changing encoding amino acid.
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[Publications] Saito, S., Inoue, T., Kawase, I., Hara, H., Tanio, Y., Tachibana, I., Hayashi, S., Watanabe, M., Matsunashi, M., Osaki, T., Masuno, T., and Kishimoto: "S. Two monoclonal antibodies against small-cell lung cancer show existence of synergism in binding" Cancer Immunol. Immunother. 33. 165-170 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Saito, S., Tanio, Y., Tachibana, I., Hayashi, S., Kishimoto, T., and Kawase, I: "Complementary DNA sequence encoding the major neural cell adhesion molecule isoform in a human small cell lung cancer cell line" Jpn. J. Cancer Res. (1993)
Description
「研究成果報告書概要(欧文)」より
