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1992 Fiscal Year Final Research Report Summary

Expression and function of neural cell adhesion molecule (N-CAM) in small cell lung cancer

Research Project

Project/Area Number 03670400
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionOsaka University

Principal Investigator

KAWASE Ichiro  The Department of internal Medicine III, Osaka University Medical School, Assistant Professor, 医学部第3内科, 講師 (10161324)

Co-Investigator(Kenkyū-buntansha) SAITO Shinichi  Osaka university Hospital, Post Doctoral Fellow, 医学部附属病院, 医員
Project Period (FY) 1991 – 1992
KeywordsSmall cell lung cancer / Neural cell adhesion molecule / N-CAM / Monoclonal antibody
Research Abstract

Murine lgG1 monoclonal antibody (mAb) designated ITK-2 was generated against a small cell lung cancer (SCLC) cell line. ITK-2 reacts with neuroendocrine tissues and tumors including SCLC, and recognize a 145kDa glycoprotein of SCLC cell membrane extracts, called neural cell adhesion molecule (N-CAM). This result was correlated with Northern blotting where a single 6.2 kb mRNA was detected in SCLC cell lines. To determine cDNA sequence encoding the N-CAM isoform, several cDNA clones were isolated from OS2-R, a SCLC cell line established in our laboratory. Based on the analysis of the full-length cDNA obtained from two clones, the sequence of this 145 kDa isoform was shown to be essentially identical to that of the 140 kDa N- CAM isoform of neuroblastoma except two differences which include the lack of 30-bp insert termed the VASE exon and a single base pair change at position 1620 without changing encoding amino acid.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Shinichi Saito: "Two monoclonal antibodies against small-cell iung cancer lines show existence of synergism in binding" Cancer Immunology Immunotherapy. 33. 165-170 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinichi Saito: "Complementary DNA seguence encoding the major neural cell adhesion mole isoform in a human small cell lung cancer cell line" Jpn.J.Cancer Res.(1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito, S., Inoue, T., Kawase, I., Hara, H., Tanio, Y., Tachibana, I., Hayashi, S., Watanabe, M., Matsunashi, M., Osaki, T., Masuno, T., and Kishimoto: "S. Two monoclonal antibodies against small-cell lung cancer show existence of synergism in binding" Cancer Immunol. Immunother. 33. 165-170 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, S., Tanio, Y., Tachibana, I., Hayashi, S., Kishimoto, T., and Kawase, I: "Complementary DNA sequence encoding the major neural cell adhesion molecule isoform in a human small cell lung cancer cell line" Jpn. J. Cancer Res. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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