1992 Fiscal Year Final Research Report Summary
Pathogenesis of summer-type hypersensitivity pneumonitis : Analysis with serotype-related polysaccharide of Trichosporon cutaneum and its nonoclonal antibodies
| Project/Area Number |
03670405
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
ANDO Masayuki Kumamoto University, First Department of Internal Medicine, Professor, 医学部, 教授 (00040204)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Summer-type hypersensitivity pneumonitis / Trichosporon cutaneum / monoclonal antibody / enzyme-linked immunosorbent assay / glucuronoxylomannan / serotype-specific polysaccharide antigen |
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| Research Abstract |
Summer-type hypersensitivity pneumonitis (SHP) is a unique type of hypersensitivity pneumonitis and the most prevalent in Japan. Our previous study clarified that the causative agent of the disease is Trichosporon cutaneum, and that the patients with SHP have high titer of antibodies against the serotype-specific antigen of polysaccharide nature which exist in the high molecular weight fraction of the culture supernatant of the yeast. In this study, we purified the serotype-specific antigen of serotype II T.cutaneum by gel filtration and affinity chromatography using a monoclonal antibody, D-8, specific for serotype II T.cutaneum, and elucidated the structure of the antigen. The affinity-purified antigen was shown to be essentially acidic polysaccharide comprising mannose, xylose, and glucuronic acid. Chemical analysis showed that this polysaccharide antigen contains a (1-3)-linked mannan backbone attached with short chains of (1-4)-linked mannose and a small portion of (1-2) linked xylose residues by substituting the 2-or4- positions of the (1-3)-linked mannose residues of the main chain. The antigenic epitope was shown to involve the terminal glucuronic acid residues as revealed by immunodiffusion test and sandwich enzyme-linked immunosorbent assay using monoclonal antibody D-8.
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