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1992 Fiscal Year Final Research Report Summary

Antigenic determinants of ovalbumin relating to hen's egg white

Research Project

Project/Area Number 03670479
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionDepartment of Pediatrics, School of Medicine, Chiba University

Principal Investigator

KOHNO Yoichi  Dept. of Pediatrics School of Medicine, associate Chiba University professor, 医学部, 助教授 (60161882)

Project Period (FY) 1991 – 1992
Keywordsfood allergy / ovalbumin / antigenic determinant / IgE antibody / IgG antibody / IgA antibody / IgG subclass / specificity
Research Abstract

Ovalbumin (OVA) is one of the major allergens in patients with food allergy. In order to delineate the antigenic determinants on OVA relating to hen's egg allergy, we studied the binding activities of IgE, IgG and IgA antibodies in patients with allergy to hen's egg white to two different OVA preparations, i.e. physically or chemically denatured OVA and enzyme-digested OVA fragments. The results obtained are as follows:
1. The binding activities of IgE antibodies to the enzyme-digested OVA fragments were different from those of IgG or IgA antibodies. In addition, whereas the binding activities of IgE and IgG antibodies to fragmented OVA were decreased, those of IgA antibodies were not decreased, compared with those to native OVA. These findings suggest that the specificities of IgE antibodies to OVA are different from those of IgG or IgA and that human IgA binding is preferentially associated with sequential protein structures. 2. Anti-OVA antibodies in sera from patients with hen's egg white were mainly associated with IgG1 and IgG4. The binding activities of IgG1 antibodies to denatured or fragmented OVA were different from those of IgG4. These findings suggest that the specificites of IgG1 antibodies to OVA are different from those of IgG4.3. IgE antibodies in sera from most patients with hen's egg allergy bind to the OVA fragment with molecular weight of 10,000 in Western blots.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 本間 季里: "IgGおよびIgE抗体により認識される卵白アルブミン、オボムコイドの抗原構造についての検討" アレルギー. 40. 1167-1175 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本間 季里: "卵白アルブミンに対するIgGサブクラス抗体の特異性" 日本小児科学会雑誌. 95. 577 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本間 季里: "卵白アルブミンに対するIgG4抗体の特異性" アレルギー. 40. 1115 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SHIMOJO,N.: "Detection of house dust mite (HDM)-specific IgE antibodies on nasal mast cells from asthmatic patients whose skin prick test and RAST are negative for HDM." Int. Arch. Allergy Immunol.98. 135-139 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本間 季里: "食物アレルギーの診断における抗原誘発直腸粘膜細胞の有用性" アレルギー. 41. 749-756 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本間 季里: "乳児喘息における血漿エンドセリン値の変動" 小児科診療. 55. 2093-2095 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下条 直樹: "カゼイン加水分解乳に感作されて消化管アレルギー反応を呈した症例における感作アレルゲンの検討" アレルギー. 41. 1413-1418 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下条 直樹: "アゼラスチンが有効であった蛋白漏出性胃腸症の一例" アレルギーの臨床. 14. 1032-1035 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本間 季里: "卵白アレルギー患者の抗OVA IgE抗体が認識するOVA抗原決定基:Western-blot法による解析" 日本小児科学会雑誌. 96. 745 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下条 直樹: "カゼイン特異的T細胞ラインの樹立と抗原決定基の解析" 第29回日本小児アレルギー学会プログラム・抄録集. 144 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下条 直樹: "IL-2産生不全を認め,臨床的に特異な経過を示した高IgE症侯群の1例" 第20回日本臨床免疫学会総会抄録集. 96 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 勝木 利行: "食物アレルゲン特異的Tcell lineの産生するサイトカインの検討" アレルギー. 41. 1018 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本間 季里: "IgA抗体が認識する卵白アルブミンの抗原構造" アレルギー. 41. 1263 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kiri Honma et al.: "Antigenic determinants on ovalbumin and ovomucoid: Comparison of the specificity of IgG and IgE antibodies." Allergy. 40. 1167-1175 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naoki Shimojo et al.: "Detection of house dust mite (HDM)-specific IgE antibodies on nasal mast cells from asthmatic patients whose skin prick test and RAST are negative for HDM." Int. Arch. Allergy Immunol. 98. 135-139 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiri Honma et al.: "Diagnosis of food allergy based on rectal mucosal cytology." Allergy. 41. 749-756 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naoki Shimojo et al.: "Allergy to casein hydrolysate formula. A study of sensitizing allergen." Allergy. 41. 1413-1418 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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