1992 Fiscal Year Final Research Report Summary
The effects of perioperative portal venous inoculation with donor lymphocytes on allograft survival in the rat.
| Project/Area Number |
03670594
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YOSHIMURA Norio Kyoto Prefectural University of Medicine Department of Surgery Assistant Professor of Surgery, 医学部, 助手 (00191643)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Organ Transplantation / Immune tolerance / Portal venous Inoculation / Immunosuppressive factor / Lymphokines |
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| Research Abstract |
Effects of perioperative portal venous inoculation of donor lymphocytes on renal allograft survival were tested in rat system. Untreated Lewis hosts rejected BN renal grafts at 7.8+0.6 days. IV administration of 1x10 BN cells to LEW hosts on day 0 caused a slight, but not significant prolongation of renal graft survival(MST=9.5+3.0 days), whereas PV inoculation of 1x10 BN cells on day 0 remarkably prolonged renal graft survival to 22.2+5.3 days(p 0.01). The prolongation of the graft survival was antigen-specific. When serum from PV-treated LEW hosts was added to MLC at a concentration of 3% of total volume, it suppressed the MLC reaction toward BN cells by 71%, but not toward third-party DA stimulators. Adoptive transfer of the serum into the virgin secondary LEW hosts significantly prolonged the graft survival of BN kidney from 7.8 to 18.9+5.5 days, but not third-party DA graft survivals(MST=7.5+0.6 days). The amounts of in vitro cytokine production of graft-infiltrating-cells(GIC) from tolerant hosts after mitogen stimulation were remarkably decreased(IL-2:8.7+1.4 U/ml, IL-3:15.4+0.6U/ml, and IL-6:24.6+3.5 U/ml) than those of uninoculated control hosts during ongoing rejection(IL-2:19.6+2.9 U/ml, IL-3:22.2+2.7 U/ml, and IL-6:67.5+13.2U/ml). The percentage of cytotoxic/suppressor T cells(OX-8) and Ia-positive cells(OX-6) in GIC cells(23.1+4.4% and 9.0+2.0%, respectively) from PV inoculated LEW hosts on day 6 postgrafting were significantly lower than those of uninoculated control recipients(39.4+8.2% and 23.0+1.9%, respectively). Cytolytic activity of GIC from tolerant hosts toward donor blastoid lymphocytes was significantly decreased(19.0+1.2%), compared with that from control allografts during ongoing rejection(51.5+5.3%). These results demonstrated that activation of both Th and Tc cells was inhibited in situ in renal allograft following administration of donor lymphocytes through the portal vein.
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[Publications] Ohsaka, Y., Yoshimura, N., Hamashima, T., Lee, C. J., Matsui, S. Hirakawa, K., and Oka, T.: "The effects of serum from hosts inoculated perioperatively with donor lymphocytes via portal vein on cytokine release in vitro" Transplantation Proceedings. 23. 559-560 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirakawa, K., Yoshimura, N., Lee, C. J., Yasui, H., Ohsaka, Y. Hamashima, T., and Oka, T.: "Impact of a perioperative portal venous administration of donor lymphocytes on survival of rat cardiac and renal graft of mongrel dog" Transplantation Proceedings. (1993)
Description
「研究成果報告書概要(欧文)」より
