1992 Fiscal Year Final Research Report Summary
Clinical and Experimental Study on Biological Parameters of Malignancy in Lung Cancer
| Project/Area Number |
03670654
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
WATANABE Yoh Kanazawa University, School of Medicine, Professor, 医学部, 教授 (20019897)
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| Co-Investigator(Kenkyū-buntansha) |
ODA Makoto Kanazawa University, School of Medicine, Assistant, 医学部, 助手 (50224241)
SHIMIZU Junzo Kanazawa University, School of Medicine, Assistant, 医学部附属病院, 助手 (50201554)
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| Project Period (FY) |
1991 – 1992
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| Keywords | primary lung cancer / DNA ploidy pattern / PCNA / biological parameter of malignancy |
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| Research Abstract |
The Cellular DNA contents, labeling index of PCNA, p53 protein expression, and number of AgNORs of lung cancer were studied in 556 resected specimens, and a correlation between the prognoses of the patients and them was evaluated. The cellular DNA contents in 1,901 propidium iodide-stained paraffin-embedded specimens were measured by flow cytometry. PCNA and p53 were also measured by immunochemical staining of paraffin-embedded specimens.Results were as followed.
1. When the DNA ploidy in primary tumors was analyzed in 556 cases, 165(29.7%) were classified as having a diploid pattern, whereas 391(70.3%) as aneuploid pattern. Furthermore, when the DNA ploidy in the metastatic sites was measured, 26 out of the 77 cases which had a diploid pattern in the primary sites, showed an aneuploid pattern in the metastatic sites. The maximum DI in the primary tumor did not correlate with that in in the metastatic tumor. These data suggests that a high incidence of heterogeneity between the primary and metastatic tumors exists. This heterogeneity was observed more frequently in adenocarcinoma than in epidermoid carcinoma. Cox's multivariate analyses clarified that DNA ploidy was a significant prognostic parameter.
2. PCNA labeling index and p53 protein positive rate of N1-3 cases and M1 cases were significantly higher, and prognoses of the cases with high PCNA-L.I. and p53 protein positive rate were significantly poorer.
3. Number of AgNORs of the tumors were higher than that of normal lung tissue. But no correlation was observed between number of AgNORs and clinicopathological factors.
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