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1993 Fiscal Year Final Research Report Summary

A Study of Growth Factor in Prostate Cancer : Effects of Bombesin and its Antibody on Growth of Human Prostate Carcinoma Cell Lines

Research Project

Project/Area Number 03670743
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionAkita University

Principal Investigator

HARADA Tadashi  Akita Univ. Sch. of Med. Dept. of Urology, Associate Professor, 医学部, 講師 (00108953)

Co-Investigator(Kenkyū-buntansha) MIYAGATA Shigeru  Akita Univ. Sch. of Med. Dept. of Urology, Assistant Professor, 医学部, 助手 (00157596)
NISHIZAWA Osamu  Akita Univ. Sch. of Med. Dept. of Urology, Associate Professor, 医学部, 講師 (60091815)
Project Period (FY) 1991 – 1993
KeywordsProstate cancer / Growth factor / Bombesin / GRP / Autocrine growth factor
Research Abstract

Bombesin(BMBS), a tetradecapeptide extracted from frog skin, was evaluated with its antibody in vitro and in vivo to evaluate growth modulating effects on the DU-145 and PC-3 human prostate carcinoma cell line. The two cell lines were maintained in DME medium containing 2% fetal calf serum and 1 mg/ml insulin in a 37^oC humidified atmosphere of 5% CO_2 and 95% air. BMBS caused a definite effect on a dose-dependent increase in DU-145 and PC-3 cell growth. The addition of antibody to BMBS, called Ab-BMBS rabbit antiserum during the lag phase of growth, resulted in the specific inhibition of DU-145 and PC-3 growth, with or without BMBS.
In in vivo experiments, nude mice were implanted with DU-145 cells in order to evaluate the suppressing effects of Ab-BMBS on carcinoma cells. After the inoculation of 107 DU-145 cells into each mouse, 20mg Ab-BMBS was intraperitoneally injected into the each mouse three times weekly, for three weeks. In mice administered with Ab-BMBS, the growth of DU-145 was markedly suppressed. By immunocytochemical study, BMBS immuno-reactivities were detected on DU-145 and PC-3 cells.
These results suggest that BMBS can function as an autocrine growth factor for human prostate carcinoma cells. Furthermore, as the result of xenografts in mice, we have learned that Ab-BMBS may inhibit the growth of human prostate carcinoma.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Harada,T.,Kigure,T.,Satoh,Y.and Shimoda,J: "In vitro and in vivo effects of bombesin and its antibody on human prostate carcinoma cell lines" Urol.int,submithing.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下田次郎,原田忠,工藤行蔵: "泌尿器科領域における細胞培養" 組織培養. 19. 107-111 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下田次郎: "Bombesinと抗bombesin抗体がヒト前立腺癌培養細胞の増殖に及ぼす影響に関する研究" 日泌尿会誌. 83. 1459-1468 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoda,J.,Harada,T.,Kigure,T.,et al.: "Growth suppressing effect of antibody versus bombesin(Ab-BMBS)for human prostatic carcinoma cell lines" J.Jpn.Soc.Cancer.Ther.27. 474-474 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimoda,J.,Koh,D.,Kigure,T.,Harada,T.,et al: "Effect of bombesin and its antibody on human prostatic cell lines(Du-145,PC-3)" J.Urol.145. 400-400 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 下田次郎,原田忠,木暮輝明,他: "ヒト前立腺癌培養細胞に対するbombesinおよび抗bombesin抗体の影響" 医学のあゆみ. 158. 759-760 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harada, T., Kigure, T, Satoh, Y.and Shimoda, J.: "In vitro and in vivo effects of bombesin and its antibody on human prostate carcinoma cell lines" Urol. int.(submitting).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda, J., Harada, T.and Kudo, K.: "Cell culture system in the research on Urology" The Tissue Culture. 19. 107-111 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda, J.: "Effects of bombesin and its antibody on human prostatic carcinoma cell lines" Jpn. J.Urol.83. 1459-1468 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda, J., Harada, T., Kigure, T., Koh, D., Yoshida, K., Yuri, Y., Miyagata, S.and Kudo, K.: "Growth suppressing effect of antibody versus bombesin (Ab-BMBS) for human prostatic carcinoma cell lines" J.Jpn. Soc. Cancer Ther.27. 474 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimoda, J., Koh, D., Kigure, T., Harada, T., Tsuchida, S.and Kudo, K.: "Effect of bombeshin and its antibody on human prostatic carcinoma cell lines (DU-145, PC-3)" J.Urol.145. 400 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinoda, J., Harada, T., Kigure, T., Koh, D., Yuri, Y., Tsuchida, S.and Kudo, K.: "Effect of Bombesin and its antibody on the growth of human prostatic carcinoma cell lines in vitro" Igakunoayumi (in Japanese). 158. 759-760 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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