1993 Fiscal Year Final Research Report Summary
Effects of endothelin-I on the function of the cultured granulosa sells
| Project/Area Number |
03670773
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KUBOTA Toshiro Tokyo Medical and Dental Univ., Lecturer, 医学部・産婦人科学, 講師 (50126223)
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| Co-Investigator(Kenkyū-buntansha) |
KAMADA Shusaku Tokyo Medical and Dental Univ., Research-Associate, 医学部・産婦人科学, 助手 (80169606)
ASO Takeshi Tokyo Medical and Dental Univ., Professor, 医学部・産婦人科学, 教授 (60093176)
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| Project Period (FY) |
1991 – 1993
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| Keywords | endothelin-1 / Granulosa cells (purcine) / cell culture / steroid hormone / cell proliferation / cyclic AMP / endothein receptor / intracellular signal transduction |
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| Research Abstract |
This study was conducted to investigate whether endothelin-1 (ET-1) has direct effects on the functions of porcine granulosa cells harvested from small or medium follicles. The positive ET-I-like immunoreactivity (ET-1-LI) was observed in the cultured porcine granulosa cells, and Northern blot analysis demonstrated the expression of messenger RNA for prepro-ET-1 in these cells. THe binding study showed the presence of non-selective, single class of binding sites which included subtype of ET_B, predominantly. Increased ET-1-LI was detected in the human follicular fluid obtained from the patients in stimulated ovarian cycles. ET-1 stimulated basal and FSH-stimulated secretion of progesterone during short term incubation (2 hrs), while it inhibited FSH- and hCG-stimulated accumulation of progesterone during long-term incubation (48 hrs) of immature or moderately mature granulosa cells. ET-1 significantly increased DNA synthesis in the cells. These biological actions were induced by ET-1, probably via cAMP - protein kinase A pathway and intracellular calcium mobilization - protein kinase C pathway. The results suggest that ET-1 exerts autocrine/paracrine effects on the porcine granulosa cell functions.
Furthermore, the present study also demonstrates that human decidual cells in the early pregnancy can synthsize and release ET-1, and possess specific receptors for ET-1 functionally coupled to phosphoinositide breakdown. This suggests that ET-1 may have its potential autocrine and/or paracrine function in human decidual cells.
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[Publications] A.Journal Kamada, S., Kubota, T., Hirata, Y., Taguchi, M., Eguchi, S., Marumo, F.and Aso, T.: "Direct effect of endothelin-I on the granulosa cells of the porcine ovary." Journal of Endocrinology. 134(1). 59-66 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Kubota, T., Kamada, S., Hirata, Y., Eguchi, S., Imai, T., Marumo, F.and Aso, T.: "Synthesis and release of endothelin-1 by human decidual cells" Journal of Clinical Endocrinology and Metabolism. 75(5). 1230-I234 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Kamada, S., Kubota, T., Hirata, Y., Imai, T., Ohta, K., Taguchi, M., Marumo, F.and Aso, T.: "Endothelin-1 is an autocrine/paracrine regulator of porcine granulosa cells." Journal of Endocrinological Investigation. 16(6). 425-431 (1993)
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[Publications] Ohta, K., Hirata, Y., Shichiri, M., Ichioka, M., Kubota, T., Marumo, F.: "Cisplatin-induced urinary endothelin excretion." JAMA. 265. 1391-I392 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Kubota, T., Taguchi, M., Kamada, S.and Aso, T.: Hormonal and immunological factors on local control of implantation in humans. In Perspectives on assisted reproduction : Frontiers in Endocrinology, Vol.4, Ares-Serono Symposia.Christengraf S.r.l., Rome, Italy, (1994)
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「研究成果報告書概要(欧文)」より
