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1992 Fiscal Year Final Research Report Summary

Origin of extrachromosome of trisomic abortuses using DNA polymorphism

Research Project

Project/Area Number 03670788
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OKAMOTO Etsuji  HIROSHIMA UNIVERSITY SCHOOL OF MEDICINE ASSOCIATE PROFESSOR, 医学部, 助教授 (00144824)

Co-Investigator(Kenkyū-buntansha) MATSUDA Hiroshi  HIROSHIMA PREFECTURAL HOSPITAL,(OBSTETRICIAN) DOCTOR, 医師 (50144465)
UEDA Katsunori  HIROSHIMA UNIVERSITY SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 医学部, 助手 (80176586)
Project Period (FY) 1991 – 1992
KeywordsTrisomy / RFLP / Chromosomal abnormality / DNA polymorphism / DNA probe
Research Abstract

The lymphocytes were obtained from Down's syndrome patients and their parents' blood by Leuco PREP, subsequently, DNAs were extracted from lymphocytes using the Phenol-chloroform method. DNAs were digested with restriction enzyme and 10mug of digested DNAs were subjected to electrophoresis on 0.8% agarose gel. After electrophoresis, DNAs in the agarose gel were transfered to nitrocellulose (nylon) filter according to the method of Southern. Probes used were : D21S16, D21S17, D21S113,D21S15,D21S19 and D21S82, which were provided by JCRB. The probe was labeled with digoxigenin and hybridization was performed. ELISA method was used to detect DNAs.
Human gametes were investigated to determinate the causal factors and mechanisms of Down's syndrome chromosomal aberrations at the sometime as the parental origin of Down's syndrome was studied.
Two hundred and ninety seven infertilized human MI oocytes from IVF (in vitro fertilization) program were studied, using the technique of Mikamo and Kamiguchi. In the 297 oocytes, 148 (49.8%) were successfully karyotyped. The abnormal rats was 21% (32/148) among which 17.7% (26/148) were aneuploids consisting of 4.8% (7/148) hyperhaploidy, 4.8% (7/148) hypohaploidy and 8.1% (12/148) diploidy. The remaining 4.0% (6/148) showed structural anomalies. Only 0.68% (1/148) was observed to be G group chromosome abnormality that from the 4.8% (7/148) hyperhaploidy which may cause Down's syndrome.
Eighteen thousand human sperm from 6 healthy man were studied by FISH (fluorescence in situ hybridization) using specific alpha-satellite DNA probe D21Z1/D13Z1. Three thousand spots were counted for each slide. Three spots which indicate disomic sperm was about 0.71% for chromosome No.21 and 13, that means No.21 chromosome disomic rate was about 0.36%.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 大浜 紘三: "Pregnancy lossをめぐる諸問題-Pre-implantation" 臨床婦人科産科. 44. 634-640 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大浜 紘三: "染色体異常と不育症" 臨床婦人科産科. 45. 32-33 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大浜 紘三: "染色体異常分娩既往者" 臨床婦人科産科. 45. 1308-1309 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大浜 紘三: "流産と先天異常" 周産期医学. 22. 1235-1239 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宇都宮 慎: "妊娠初期自然流産に関する免疫学的ならびに細胞遺伝学的研究" 日本産科婦人科学会雑誌. 44. 1413-1420 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 大浜 紘三: "流産児の染色体異常" 臨床婦人科産科. 47. 23-25 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 水之江 知哉: "Fluorescence in situ Hybridization (FISH)法を用いた出生前診断の検討" 産科と婦人科. 60. 405-408 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中岡 義晴: "ヒト未受精卵の染色体分析" 日産婦中国四国合同地方部会雑誌. 41. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 水之江 知哉: 日産婦中国四国合同地方部会雑誌. 41. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koso OHAMA: "Problem of pregnancy loss - pre-implantation" Clinical Gynecology and Obstetrics. Vol.44. 634-640 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koso OHAMA: "Chromosomal abnormality and infertility" Clinical Gynecology and Obstetrics. Vol.45. 32-33 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koso OHAMA: "Previous delivery of chromosomal abnormal infant" Clinical Gynecology and Obstetrics. Vol.45. 1308-1309 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koso OHAMA: "Spontaneous abortion and congenital anomaly" Perinatal Medicine. Vol.22. 1235-1239 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi UTSUNOMIYA: "Immunological and cytogenetic studies on early spontaneous abortions" Acta Obst. Gynaec. Jpn. Vol.44. 1413-1420 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koso OHAMA: "Chromosomal abnormalities in spontaneous abortuses" Clinical Gynecology and Obstetrics. Vol.47. 23-25 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomoya MIZUNOE: "Prenatal studies of interphase cells by fluorescence in situ hybridization (FISH)" Obstet. Gynecol. <Tokyo>. Vol.60. 405-408 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshiharu NAKAOKA: "Chromosomal analysis in human infertilized oocytes" The Chugoku & Sikoku Districts Journal of the Japan Society of Obstetrics and Gynecology. Vol.41. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomoya MIZUNOE: "Studies of new born chromosomal aneuploidy rate using FISH method with alpha satellite probe" The Chugoku & Sikoku Districts Journal of the Japan Society of Obstetrics and Gynecology. Vol.41. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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