1993 Fiscal Year Final Research Report Summary
Immunological study of head and neck cancer
| Project/Area Number |
03670820
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Tokai University School of Medicine |
|---|
Principal Investigator |
MIYAKE Hirosato Tokai Univ., E.N.T., Professor., 医学部・耳鼻咽喉科, 教授 (60055720)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hideshige Tokai Univ., E.N.T., Asistant., 医学部・耳鼻咽喉科, 助手 (80054039)
YAMAUCHI Susumu J.college of N.M.T., Asso.Prof., 医療技術短期大学, 助教授 (50166576)
|
|---|
| Project Period (FY) |
1990 – 1993
|
| Keywords | : Head and neck cancer / Tumor marker / Immunohistochemical detection / IEP / False positive / Anti SCC associated antibody / Commercial antibodies |
|---|
| Research Abstract |
A novel squamous cell cancer associated antibody (SCCAAb) was developed, since detection of SCC associated antigens (SCCAA) against the cells and tissues of SCC of head and neck is highly effective for early diagnosis.
SCCAAb was prepared by immunization to rabbit with specific components from pharyngeal SCC cell Line.
Western blot analysis revealed that SCCAA consist of four components from 120KD to 225KD.
Immunological technique by ABC was applied and SCCAAb, commercial anti-bodies ( Involcrin, Keratin, EGF-R, EMA, PKK2, K-ras (P21) ) were used as first antibodies.
Following results were obtained.
Positive number and percent were 34/40 (85%) with SCCAAb, 28/34 (82%) with Involcrine, 29/30 (97%) with Kratin, 26/39 (67%) with EGF-R, 25/37 (68%) with EMA, 26/37 (70%) with PKK2, and 15/37 (1%) with K-ras (P21). False positive number and percent in benign specimens, 11% with SCCAAb, 42% with Involcrine, 85% with Kratin, 34% with EGF-R 28% with EMA, 49% with PKK2 and 18% with K-ras (P21), respectively.
|
