• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Basic Research of Light-Evoked Changes in the Interphotoreceptor Matrix

Research Project

Project/Area Number 03670834
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Ophthalmology
Research InstitutionKagoshima University

Principal Investigator

UEHARA Fumiyuki  Kagoshima Univ., Fac. of Med. Hospital, Assist. Prof., 医学部附属病院, 講師 (30168653)

Co-Investigator(Kenkyū-buntansha) UNOKI Kazuhiko  Kagoshima Univ., Fac. of Med. Hospital, Research Assoc., 医学部附属病院, 助手 (60193926)
SAMESHIMA Munefumi  Kagoshima Univ., Fac. of Med., Research Assoc., 医学部, 助手 (80041333)
Project Period (FY) 1991 – 1992
KeywordsInterphotoreceptor matrix / Light-evoked changes / Retinal degeneration / Sialic acid / alpha2,6-sialyltransferase / Messenger RNA / Electrical resistance
Research Abstract

(1) The light-evoked changes of the interphotoreceptor matrix (IPM) were observed in green- and blue-light-adapted rats, whereas they were not detected in red-light-adapted animals. The results indicate that rod photoreceptors are predominantly responsible for light-evoked changes in the IPM.
(2) By electron microscopic examination, the granular components of the IPM were diffusely detected around outer segments in dark-adapted rats, whereas they were observed to be accumulated on the apical process of the retinal pigment epithelia in light-adapted animals. The light-evoked change of the IPM is proved to occur on electron microscopic level.
(3) The light-evoked changes of the IPM were observed to be extinct when the outer segments became moderately or markedly shortened in mice with inherited retinal degeneration. The results indicate that the presence of normal outer segments is necessary for the light response of the IPM to occur.
(4) By in situ hybridization histochemistry using antisense RNA probes complementary for alpha2,6-sialyltransferase mRNA, its expression in rat retinas was increased between post-natal day (P) 14 and P16, when the light-evoked change in the IPM first occurs. The results indicate that the presence of sialic acid on the termini of sugar chains plays an important role in generating the light responece of the IPM.
(5) The electrical resistance of the IPM of the monkey retina was measured. The resistance of the peripheral retina was lower than that of the macula, suggesting that the electrical resistance of the rod associated IPM is lower than that of the cone associated IPM.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 上原 文行: "Developmental change of distribution of β-galactoside α2,6-sialyltransferase mRNA in rat retina" Experimental Eye Research. 56. 89-93 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中嶋 康幸: "Interphotoreceptor matrix in the colored-light-adapted rat" Ophthalmic Research. 24. 308-315 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "遺伝性視細胞変性網膜光受容体間基質分布の光応答" 日本眼科学会雑誌. 96. 473-478 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "持続光起因性変性網膜の光受容体間基質II.光応答の組織化学的検索" あたらしい眼科. 9. 1553-1555 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "遺伝性視細胞変性網膜のムチン型複合糖質" あたらしい眼科. 9. 1619-1622 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "Interphotoreceptor matrixと網膜変性とくにその光応答との関係について" 医学のあゆみ. 163. 142- (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "Distribution of peripherin/rds mRNA in cone-dominant squirrel retina" Experinental Eye Research. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 柳田 豊子: "Demonstration of pertpherin/rds mRNA in normal and light-damaged rat retinas by in situ hybridization histochomistry" Japanese Journal of Ophthalmology. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "Fixative sultable for in situ hybridization histochemistry" The Journal of Histochemistry and cytochemistry. (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 上原 文行: "Current Aspects in Ophthalmology(Roles of sialoglycocorjugates in the rod-associated interphotoreceptor matrix)" Elsevier Science Publishers, 4(907-910) (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uehara, F., Ohba, N., Nakashima, Y., Yanagita, T. Ozawa, M., Muramatsu, T.: "Developmental change of distribution of beta-galactoside alpha2,6-sialyltransferase mRNA in rat retina" Experimental Eye Research. 56-1. 89-93 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakashima, Y., Uehara, F., Ohba, N.: "Interphotoreceptor matrix in the colored-light-adapted rat" Ophthalmic Research. 24-5. 308-315 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N., Yasumura, D., LaVail, M. M.: "Light response of the interphotoreceptor matrix in inherited degenerative retina" Acta Soc. Ophthalmol. Jpn.96-4. 473-478 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N., LaVail, M. M.: "Light response of the interphotoreceptor matrix in constant light-exposed mouse retina" J. Eye (Atarashii Ganka). 9-9. 1553-1555 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N., LaVail, M. M.: "Mucin type glycoconjugates in the inherited degenerative mouse retina" J. Eye (Atarashii Ganka). 9-9. 1619-1622 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N.: "Interphotoreceptor matrix and retinal degeneration in relation to the light response of the interphotoreceptor matrix" J. Clinical & Experimental Medicine (IGAKU NO AYUMI). 163-2. 142 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N., Nakashima, Y., Yanagita, T. Ozawa, M., Muramatsu, T.: "Distribution of peripherin/rds mRNA in cone -dominant squirrel retina Experimental Eye Research" Experimental Eye Research.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanagita, T., Uehara, F., Nakashima, Y., Ozawa, M. Muramatsu, T., Ohba, N.: "Demonstration of peripherin/rds mRNA in normal and light-damaged rat retinas by in situ hybridization histochemistry" Japanese Journal of Ophthalmology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N., Nakashima, Y., Yanagita, T. Ozawa, M., Muramatsu, T.: "Fixative suitable for in situ hybridization histochemistry" The Journal of Histochemistry and Cytochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uehara, F., Ohba, N., Unoki, K., Yasumura, D. LaVail, M. M.: "Roles of sialoglycoconjugates in the rod-associated interphotoreceptor matrix" Current Aspects in Ophthalmology. 907-910 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1994-03-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi