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1992 Fiscal Year Final Research Report Summary

The Adhesion molecule expression in osteoclastic differentiating process

Research Project

Project/Area Number 03670969
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

MITANI Hideo  TOHOKU UNIVERSITY,SCHOOL OF DENTISTRY,PROFESSOR, 歯学部, 教授 (50014220)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Yoshiuki  TOHOKU UNIVERSITY,SCHOOL OF DENTISTRY,LECTURER, 歯学部, 講師 (20187470)
SHIMIZU Yoshinobu  TOHOKU UNIVERSITY,SCHOOL OF DENTISTRY,ASSOCIATE PROFESSOR, 歯学部, 助教授 (20005078)
Project Period (FY) 1991 – 1992
KeywordsOSTEOCLAST / MAC-1 / LFA-1 / CD 44 / MEL 14 / ICAM-1 / FACS / IMMUNOHISTOCHEMISTRY
Research Abstract

The purpose of this investigation was to reveal the expressions of adhesion molecules on the surface of osteoclast during osteoclastic differentiation in vivo and in vitro. Bone marrow cells were cultured with the co-culture fluid of osteoblasts and bone marrow cells and divided to adherent cells and non adherent cells at 1,3,5 days.Surface antigens (Mac-1,LFA-1,CD 44 and Mel 14) of the cultured bone marrow cells were analyzed by Flow cytometry (FACS) and also the cultured bone marrow cells were stained with tartrate resistent acid phosphatase (TRAP) on 14 days culture. In order to evaluate the expressions of adhesion molecules (Mac-1,LFA-1,ICAM-1) in peridontal tissues of mice, immunohistochemical staining in vivo were made.
The following results were obtained:
In vitro experiments,adhesion molecules (Mac-1,LFA-1,ICAM-1) of osteoclasts converted to weaker expression than that of macrophages in bone marrow cells cultured with the coculture fluid. And then in vivo experiments, Mac-1 and LFA-1 positive cells could be recognizes in the inside of periodontal ligaments and the marrow spaces of mice. ICAM-1 positive cells were observed specifically around the apex of the tooth root and marrow spaces. However,when orthodontic force were applied to teeth, in compressed side of alveolar bone, both numbers of osteoclast and the adhesion molecule positive cells were significantly increased.
These results suggested that the relationship between the expressions of adhesion molecules and the differentiation of osteoclasts was very important for the maturation process of osteoclast from hematopoietic stem cells.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 後藤 安史他: "骨髄細胞、脾細胞、末梢血における破骨細胞の分化誘導過程と骨芽細胞の調節因子について" 日本骨代謝学会雑誌. 9. 184 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤 安史他: "骨髄細胞,脾細胞,末梢血に対する破骨細胞誘導因子の作用について" 日本矯正歯科学会雑誌. 51. 262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義之他: "歯の移動時の歯周組識の改造変化におけるマクロファージ系細胞の動態に対する歯根膜細胞の役割について" 歯科基礎医学会雑誌. 33. 123 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤 安史,他: "破骨細胞の分化誘導過程における接着因子の発現様相について" 日本骨代謝学会雑誌. 10. 230 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田上 篤,他: "細胞培養実験から見た歯根膜の特性" 日本腔インプラント学会誌. 196 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 清水 義信,他: "人工歯根修復における歯根膜の分化機能" 日本腔インプラント学会誌. 48-49 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田上 篤他: "歯根修復に関する研究第6報歯根膜細胞の産出する破骨細胞誘導抑制因子とその性状" 日本保存学会雑誌. 35. 14 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotoh,Y.,Chiba,M.,Shimizu,Y.,Shimizu,Y.,Mitani,H.: "The regulation factor of osteoblasts on the formation of osteoclast derived from bone marrow cells, splenocytes and peripheral blood leukocytes." J Bone Mine Metab. 9S. 184 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotoh,Y.,Shimizu,Y.,Chiba,M.,Mitani,H.:"Reaction of osteoclasts differentiating factor on bone marrow cells, splenocytes and peripheral blood lymphocytes" J Jpn Orthod Sci. 51. 262 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu,Y.,Chiba,M.,Kamo,N.,Gotoh,Y,Shimizu,Y.,Mitani,H.: "The regulation of macrophages in responses of periodontal tissues by periodontal ligament cells during tooth movement." Jpn J Oral Biol. 33S. 123 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotoh,Y.,Shimizu,Y.,Mitani,H.: "Adhesion molecule expression in differentiation process of osteoclast." J Bone Mine Metab. 10S. 96 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimizu,Y.: "The differentiation of periodontal ligament requesting for an artificial root restoration." J.J.S.O.I.Suppl. 48-49 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tagami,A., Shimizu,Y., Okuda,R.: "Characteristics of periodontal ligament cells and its culture on various surfaces of titanium." J.J.S.O.I.Suppl. 196 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tagami,A., Shimizu,Y., Okuda,R.: "An artificial restration of dental root-The suppressing activity of periodontal ligament cells on the formation of osteoclast derived from peripheral blood lymphocytes." Japan J. Conserv. Dent. 35. 14 (1992)

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Published: 1994-03-24  

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