1992 Fiscal Year Final Research Report Summary
The Adhesion molecule expression in osteoclastic differentiating process
| Project/Area Number |
03670969
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
MITANI Hideo TOHOKU UNIVERSITY,SCHOOL OF DENTISTRY,PROFESSOR, 歯学部, 教授 (50014220)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Yoshiuki TOHOKU UNIVERSITY,SCHOOL OF DENTISTRY,LECTURER, 歯学部, 講師 (20187470)
SHIMIZU Yoshinobu TOHOKU UNIVERSITY,SCHOOL OF DENTISTRY,ASSOCIATE PROFESSOR, 歯学部, 助教授 (20005078)
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| Project Period (FY) |
1991 – 1992
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| Keywords | OSTEOCLAST / MAC-1 / LFA-1 / CD 44 / MEL 14 / ICAM-1 / FACS / IMMUNOHISTOCHEMISTRY |
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| Research Abstract |
The purpose of this investigation was to reveal the expressions of adhesion molecules on the surface of osteoclast during osteoclastic differentiation in vivo and in vitro. Bone marrow cells were cultured with the co-culture fluid of osteoblasts and bone marrow cells and divided to adherent cells and non adherent cells at 1,3,5 days.Surface antigens (Mac-1,LFA-1,CD 44 and Mel 14) of the cultured bone marrow cells were analyzed by Flow cytometry (FACS) and also the cultured bone marrow cells were stained with tartrate resistent acid phosphatase (TRAP) on 14 days culture. In order to evaluate the expressions of adhesion molecules (Mac-1,LFA-1,ICAM-1) in peridontal tissues of mice, immunohistochemical staining in vivo were made.
The following results were obtained:
In vitro experiments,adhesion molecules (Mac-1,LFA-1,ICAM-1) of osteoclasts converted to weaker expression than that of macrophages in bone marrow cells cultured with the coculture fluid. And then in vivo experiments, Mac-1 and LFA-1 positive cells could be recognizes in the inside of periodontal ligaments and the marrow spaces of mice. ICAM-1 positive cells were observed specifically around the apex of the tooth root and marrow spaces. However,when orthodontic force were applied to teeth, in compressed side of alveolar bone, both numbers of osteoclast and the adhesion molecule positive cells were significantly increased.
These results suggested that the relationship between the expressions of adhesion molecules and the differentiation of osteoclasts was very important for the maturation process of osteoclast from hematopoietic stem cells.
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[Publications] Gotoh,Y.,Chiba,M.,Shimizu,Y.,Shimizu,Y.,Mitani,H.: "The regulation factor of osteoblasts on the formation of osteoclast derived from bone marrow cells, splenocytes and peripheral blood leukocytes." J Bone Mine Metab. 9S. 184 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Shimizu,Y.,Chiba,M.,Kamo,N.,Gotoh,Y,Shimizu,Y.,Mitani,H.: "The regulation of macrophages in responses of periodontal tissues by periodontal ligament cells during tooth movement." Jpn J Oral Biol. 33S. 123 (1991)
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「研究成果報告書概要(欧文)」より
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