1992 Fiscal Year Final Research Report Summary
Biochemical Studies on neurotransm tter transporters in syhoptic vesica
| Project/Area Number |
03671049
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Osaka University |
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Principal Investigator |
MONYAMA Yoshinori Istitrte of Sciontitic & Industrial Research Research assciate, 産業科学研究所, 助手 (10150658)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Neuron / Chemical tramsmission / Synaptic vesicle / V-ATPase / Proton Pump / Transnitter / Transport |
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| Research Abstract |
Synaptic vesicles play an important role in neuronal transmission in nerve termini. They accumulate high concentrations of neurotransmitters and , upon receiving the proper signal, secrete them by exocytosis. We found that vacuolar ATPase on the vesicles forms an electrochemical gradient of protons across the membranes and transmitters are taken up through specific transporters coupled with this gradient. However, little is known about biochemical properties on the transporters. This study was focused to elucidate some biochemical properties on the transporter and vacuolar ATPase. During the two years, we obtained following results. (1) Energy coupling of glutamate transporter, the transporter is driven by membrane potential but other transporters are driven by DELTApH. (2) We established a procedure for reconstitution of transporter with bacterial F-ATPase. The procedure can be used for the assay system for purification of transporter. (3) We identified dibenzylglutamate as potent inhibitor for glutamate transporter. (4) We found that synaptic vesicle dopamine transpoter recognized methlphenylpyridinium (MPP^+), as a substrate. These results give basic information for the further biochemical study of transmitter transporters.
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[Publications] Johnouchi, M., Takeyama, M., Chaiprasert, P., Noumi, T., Moriyama, Y., Maeda, M., and Futai, M: "Esherichia coli H^+ - ATPase: Role of the epsilon Subunit for Binding of F1 to F0 Sector" Arch. Biochem. Biophys. 292. 376-381 (1992)
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[Publications] Johnouchi, M., Takeyama, M., Noumi, T., Moriyama, Y., Maeda, M., and Futai, M: "Role of the Amino Terminal Region of the delta Subunit of Escherichia coli H^+ - ATPase (F0F1)" Arch. Biochem. Biophys. 292. 87-94 (1992)
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[Publications] Inohara, N., Iwamoto, A., Moriyama, Y., Shinohara, S., Maeda, M., and Futai, M: "Two Genes, atpC1 and atpC2, for the gamma Subunit of Arabidopsis thaliana" J. Biol. Chem. 266. 7333-7338 (1991)
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