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1992 Fiscal Year Final Research Report Summary

Studies on Species-specific Expression of Uricase Gene

Research Project

Project/Area Number 03671068
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionToho University

Principal Investigator

MOTOJIMA Kiyoto  Toho University school of Phemaceutical Sciences Associate Professor, 薬学部, 助教授 (70166338)

Co-Investigator(Kenkyū-buntansha) GOTO Sataro  Toho University School of Phemaceutical Sciences Professor, 薬学部, 教授 (10012650)
Project Period (FY) 1991 – 1992
KeywordsUricase / Urate Oxidase / Species-specific expression / Chromosomal Gene / Gene Organization / Peroxisome
Research Abstract

To study the genetic background for species- and tissue-specific expression of the uricase gene, the structures of the chromosomal uricase genes were studied Most studies were concentrated on the rabbit gene because rabbit has two uricase mRNA species and they are different only in the 5'-non-coding region. Two mRNAs are expressed in the liver and also in the kidney and the brain in a tissue-specific manner. A part of the rabbit chromosomal gene for uricase was cloned and the structures of 4 exons were determined. The organization of the gene perfectly matched with that of the corresponding rat gene. To clone the transcriptional regulatory region of the rabbit gene, extensive screening of genomic libraries with cDNA probes was carried out and then a PCR method was employed, but we unfortunately could not obtain the sequence.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 本島 清人: "ペルオキシソーム酵素ウリカーゼの発現特異性" 生体の科学. 42. 198-200 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] 本島 清人: "ペルオキシソーム増殖剤レセプター" ファルヌシア. 27. 1175-1176 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] Oshiman,K.et al.: "Control region and gastric specific transcription of the rat H^+,K^+-ATPase subunit gene" FEBS Letters. 282. 250-254 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawanomoto,K.et al.: "cDNA cloning and segueace of rat riboruclease inhibitor,and tissue distribution of the mRNA" Biochim.Biophyo.Acta. 1129. 335-338 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Motojima,K.et al.: "cDNA cloring for and preparation of antibodies against suburitd of H^+-ATPase in rat mitoch ordria" Biochem.Biophys.Res.Commun.182. 1130-1138 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Nezu,J.et al.: "Mokaular cloring of a rat liven cDNA encoding the 16kpa subunit of uacablan H ATPases:Organellan and tissue distribation of 16 kDa proteolipids" J.Biochem.112. 212-219 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Motojima,K.et al: "Rat liven Bip/GRP78 is down-regalated by a peroxisone-proliferafor,clotibrate" FEBS Letlers. 308. 207-210 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Motojima,K.et al.: "Specific repression of tranthyrefia gene expression in rat liven by a peroxisone praliferator clofibrate" Biochem.Biophys.Res.Commun.188. 799-806 (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Motojima,K.et al.: "A Protein histidiar lcirase induced in rat liven by perorisore proliferators:In vitro activation by Ras protein and guanine nucleotides" FEBS Letlers. (1993)

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      「研究成果報告書概要(和文)」より
  • [Publications] Motojima,K.: "Species- and tissue-specific expression of peroxisomal uricase (in japanese)" Seitainokagaku. 42. 198-200 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motojima,K.: "Peroxisome proliferator receptor (in japanese)" Farumashia. 27. 1175-1176 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Oshiman,K., Motojima,K. , Mahmood,S. , Shimada,A. ,Tamura,S. , Maeda,M. ,and Futai,M.: "Control region and gastric specific transcription of the rat H^+, K^+-ATPase = subunit gene" FEBS Letters. 281. 250-254 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawanomoto,K., Motojima,K., Sasaki,M., Hattori,H., and Goto,S.: "cDNA coning and sequence of rat ribonuclease inhibitor, and tissue distribution of the mRNA" Biochim. Biophys. Acta. 1129. 335-338 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Motojima,K. and Imanaka,T.: "cDNA cloning for and preparation of antibodies against subunit d of H^+-ATP synthase in rat mitochondria" Biochem. Biophys. Res. Commun. 182. 1130-1138 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nezu,J., Motojima,K., Tamura,H. and Ohkuma,S.: "Molecular cloning of a rat liver cDNA encoding the 16 kDa subunit of vacuolar H^+-ATPases: Organellar and tissue distribution of 16 kDa proteolipids" J.Biochem.112. 212-219 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motojima,K. and Goto,S.: "Rat liver Bip/GRP78 is down-rregulated by a peroxisome-proliferator, clofibrate" FEBS Lett.308. 207-210 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motojima,K., Goto,S. and Imanaka,T.: "Specific repression of transthyretin gene expression in rat liver by a peroxisome proliferator clofibrate" Biochem. Biophys. Res. Commun.188. 799-806 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Motojima,K. and Goto,S.: "A protein histidine kinase induced in rat liver by peroxisome proliferators : In vitro activation by Ras protein and guanine nucleotides" FEBS Letters. (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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