1992 Fiscal Year Final Research Report Summary
Characterizatin of rdw Rat as a New Animal Model for Hereditary Dwarfism
| Project/Area Number |
03680042
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Kitasato University |
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Principal Investigator |
FURUDATE Senichi Kitasato University. Sch. of Medicine. Lecturer, 医学部, 講師 (80095512)
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| Co-Investigator(Kenkyū-buntansha) |
MUTO Takeshi 北里大学, 医学部, 教授 (90072884)
OHYAMA Yoshihide 北里大学, 医学部, 講師 (00104547)
ONO Masao 北里大学, 医学部, 講師 (40050645)
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| Project Period (FY) |
1991 – 1992
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| Keywords | rdw rat / Dwarfism / Pituitary / Pit-l / Thyroid / Pituitary hormones / Thyroid hormones / Thyrotropin (TSH) |
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| Research Abstract |
The rdw rat (gene symbol; rdw) with hereditary dwarfism has been shown immunohistochemically to have subnormal numbers not only of growth hormone (GH) but also of prolactin and thyrotrophin-positive cells. To characterize the dwarfism of this strain, the expression of pituitary hormone mRNAs was examined by Northern hybridization. The pituitary gland in the rdw rat expressed 30-100 times fewer GH and prolactin mRNAs than that of the normal controls, whereas mRNAs for proopiomelanocortin and the alpha subunit of rat glycoprotein hormone revealed a significant increase. There was a slight difference in rat luteinizing hormone-beta subunit and follicle-stimulating hormone-b subunit between normal and rdw rats. The suppressed expression of a pituitary-specific transcription factor, pit-1, is considered to cause hereditary dwarfism in mouse strains, Snell and Jackson, whose phenotypes resemble those of rdw rat. In this study, however, no difference in mRNA expression for pit-l was found between rdw rats and controls. This work indicates that rdw rat may not have the same genotype as the phenotypically similar dwarf mice, the snell, Jackson and Ames.
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