1992 Fiscal Year Final Research Report Summary
Biochemical characterization of endothelin converting enzyme
| Project/Area Number |
03680136
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Chiba University |
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Principal Investigator |
KIMURA Sadao Chiba University, School of Medicine, Professor, 医学部, 教授 (40134225)
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| Project Period (FY) |
1991 – 1992
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| Keywords | endothelin / big endothelin / endothelin converting enzyme / phosphoramidon |
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| Research Abstract |
1. Two phosphoramidon sensitive-neutral metalproteases (M1 and M2) with endothelin converting activity were detected in membranous fractions of porcine lung. M1 specifically converts only big ET-1 to ET-1 not big ET-2 and big ET-3 to ET-2 and ET-3, respectively. Taking together with other biochemical analyses, it is suggested that M1 is a new physiological enzyme and M2 may be a known neutral endopeptidase
2. (i) Analysis of endothelin-2 (ET-2) biosynthetic pathway showed that ET-2 is produced via big ET-2(1-38) as an intermediate form. The biochemical characterization of ET-2 converting enzyme (ECE-2) indicates that ECE-2 is a phosphoramidon-sensitive metal protease and very similar to endothelin-1 converting enzyme (ECE-1) from endothelial cells.
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[Publications] Sawamura, T., Shinmi, O., Kishi, N., Sugita, Y., Yanagisawa, M., Goto, K., Masaki, T., Kimura, S.: "Characterization of phosphoramidon-sensitive metalloproteinases with endothelin-converting enzyme activity in porcine lung membrane." Biochim. Biophys. Acta. 1161. 295-302 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Yorimitsu, K., Shinmi, O., Nishiyama, M., Moroi, K., Sugita, Y., Saito, T., Inagaki, Y., Masaki, T., Kimura, S.: "Effect of phosphoramidon on big endothelin-2 conversion into endothelin-2 in human renal adenocarcinoma (ACHN) cells. Analysis of endothelin-2 biosynthetic pathway." FEBS Lett.314. 395-398 (1992)
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「研究成果報告書概要(欧文)」より
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[Publications] Sawamura, T., Kasuya, Y., Matsushita, Y., Suzuki, N., Shinmi, O., Kishi, N., Sugita, Y., Yanagisawa, M., Goto, K., Masaki, T., Kimura, S.: "Phosphoramidon inhibits the intracellular conversion of big endothelin-1 to endothelin-1 in cultured endothelial cells." Biochem. Biophys. Res. Commun.174. 779-784 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishikawa, T., L. Li, Shinmi, O., Kimura, S., Yanagisawa, M., Goto, K., Masaki, T.: "Characteristics of binding of endothelin-1 and endothelin-3 to rat hearts." Circ. Res.69. 918-926 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Shigeno, T., Mima, T., Yanagisawa, M., Saito, A., Fujimori, M., Shiba, R., Goto, K., Kimura, S., Yamashita, K., Yamasaki, Y., Masaki, T., Takakura, K.: "Possible role of endothelin in the pathogenesis of cerebral vasospasm." J. Cardiovasc. Pharmacol.17. S480-S483 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Giaid, A., Masaki, T., Ouimet, T., Yanagisawa, M., Gaspar, L., Cantin, M., Kimura, S., Castellucci, V.F.: "Expression of endothelin-like peptide in the nervous system of the marine mollusk Aplysia." J. Cardiovasc. Pharmacol.17. S449-S451 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Saito, A., Shiba, R., Yanagisawa, M., Masaki, T., Kimura, S., Yamada, K., Mima, T., Shigeno, T., Goto, K.: "Characterization of the effect of endothelins in canine cerebral arteries." J. Cardiovasc. Pharmacol.17. S219-S221 (1991)
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「研究成果報告書概要(欧文)」より
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[Publications] Tomobe, Y., Ishikawa, T., Yanagisawa, M., Kimura, S., Masaki, T., Goto, K.: "Mechanism of altered sensitivity to endothelin-1 between aortic smooth muscles of spontaneously hypertensive and wister-kyoto rats." J. Pharmacol. Exp. Ther.257. 555-561 (1991)
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[Publications] Saito, A., Shiba, R., Yanagisawa, M., Masaki, T., Kimura, S., Yamada, K., Mima, T., Shigeno, T., K. Goto: "Endothelins: vasoconstrictor effects and localization in canine cerebral arteries." Br. J. Pharmacol.103. 1129-1135 (1991)
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[Publications] Sakurai, T., Yanagisawa, M., Inoue, A., Ryan, U., Kimura, S., Mitsui, Y., Goto, K., Masaki, T.: "cDNA cloning, sequence analysis and tissue distribution of rat preproendothelin-1 mRNA." Biochem. Biophys. Res. Commun.175. 44-47 (1991)
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「研究成果報告書概要(欧文)」より
