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1992 Fiscal Year Final Research Report Summary

A study on the mechanism of interaction of bone marrow-derived immature thymocytes with thymic dendritic cells

Research Project

Project/Area Number 03680140
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionThe University of Tokyo,

Principal Investigator

IWAMORI Masao  University of Tokyo,Associate Prof., 医学部(医), 助教授 (90110022)

Project Period (FY) 1991 – 1992
Keywordsganglioside / steroid / thymocyte / uterine endometrium / sulfotarnsferase / sialyltransferase / II^3NeuAc-LacCer / cholesterol sulfate
Research Abstract

A characteristic disappearance of thymocytes from the cortex of thymus was observed 48 hrs after intraperitoneal injection of cortisone acetate and the same alteration was also observed by radiation with 500rad of X-ray. However, immnosuppressive agents, cyclosporin A and FK 506 did not cause the reduction of the cortical thymocytes, with a slight loss of medullary thymocytes by FK 506. Reduction of the cortical thymocytes resulted in a reduced concentration of IV^3NeuGc-nLc_4Cer and VI^3NeuGc-nLc_6Cer, both of which were the marker gangliosides of cortisone-sensitive cortical thymocytes. At the same time, a significant increase in II^3NeuAc-LacCer, in both total amount and concentartion, was observed in both rabbit and mouse thymuses after cortisone administration. The specific activity of sialyltransferase, with LacCer and CMP-^<14>C-NeuAc as substrates, was the same in the thymus homogenates of cortisone-administered and control mice. Moreover, no difference was observed in the capacity to synthesize II^3NeuAc-LacCer from ^<14>C-ManNAc in the loose thymuses of cortisone-administered and control mice. However, sialyltransferase involved in the synthesis of II^3NeuAc-LacCer with CMP-^<14>C-NeuAc was only detected in the loose thymuses after cortisone-administration, that is, not in the controls, indicating that LacCer on the cell surface is characteristically sialylated by the plasma membrane-associated sialyltransferase only after cortisone administration. Since the cortisone-induced synthesis of II^3NeuAc-LacCer and activation of the sialyltransferase seemed to occur in thymus matrix cells, these observations might be related to the mechanism underlying detachment of cortisone-sensitive lymphocytes from the thymus matrix cells on modification of the lymphocyte receptor molecule.
The analogical hormone-dependent regulation of a negative charge was observed in rabbit uterine endometrium after steroid administration.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] HORIKAWA K,NAKAKUMA H,NAGAKURA.S,IWAMORI M,TAKATSUKI K: "Hemolysis of human erythrocytes is a new bioactivity of gangliosides" Journal of Experimental Medicine. 174. 1385-1391 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MIKAMI M,NOZAWA S,IWAMORI M,NAGAI Y: "Menstrual cycle-associated expression of 2-hydroxy fatty acyl phytosphingosine containing GlcCer,LacCer and Gb3Cer in buman uterine endometrium" Biochimica Biophysica et Acta. 1125. 104-109 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KUBUSHIRO K,TSUKAZAKI K,TANAKA J,KIGUCHI K,IWAMORI M: "Human uterine endometrial adenocarcinoma-characteristic acquirement of synthetic potential for II^3SO_3-LacCer and ganglio-series sulfoglycosphingolipids" Cancer Research. 52. 803-809 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KIGUCHI K,NOZAWA S,IWAMORI M,NAGAI Y: "Glycosphingolipids of human various ovarian tumors:A significantly high expression of I^3SO_3-GalCer and Lewis antigen in musinous cystadenocarcinoma" Cancer Research. 52. 416-421 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SAITOH T,SUGANO K,NATOMI H,Zhao W,IWAMORI M,YAZAKI Y: "Glycosphingolipid receptors in human gastric mucosa for Helico bacter pylori" Europian Journal of Gastroenterogy and Hepatology. 4. 49-53 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KUSUNOKI S,INOUE K,IWAMORI M,NAGAI Y,MANNEN T,KANAZAWA I: "Developmental changes of fucosylated glycoconjugates in rabbit dorsal root ganglia" Neuroscience Research. 15. 74-80 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horikawa,K, Nakakuma H, Nagakura S, Iwamori M, Takatsuki K:"Hemolysis of human erythrocytes is a new bioactivity of gangliosides." Journal of Experimental Medicine. 174. 1385-1391 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikami M, Nozawa S, Iwamori M, Nagai Y:"Menstrual cycle-associated expression of 2-hydroxy fatty acyl phytosphingosine containing GlcCer, LacCer and Gb3Cer in human uterine endometrium." Biochimica Biophysica et Acta. 1125. 104-109 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubushiro K, Tsukazaki K, Tanaka J, Kiguchi K, Iwamori M: "Human uterine endometrial adenocarcinoma-characteristic acquirement of synthetic potential for II3S03-LacCer and ganglio-series sulfoglycosphingolipids." Cancer Research. 52. 803-809 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kiguchi K, Nozawa S, Iwamori M, Nagai Y: "Glycosphingolipids of human various ovarian cancers; A significantly high expression of I3S03-GalCer and Lewis antigen in mucinous cystadenocarcinoma." Cancer Research. 52. 416-421 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saitoh T, Sugano K, Natomi H, Zhao W, Iwamori M, Yazaki Y: "Glycosphingolipid receptors in human gastric mucosa for Helicobacter pylori." Europian Journal of Gastroenterogy and Hepatology. 4. 49-53 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusunoki S, Inoue K, Iwamori M, Nagai Y, Mannen T, Kanazawa I: "Developmental changes of fucosylated glycoconjugates in rabbit dorsal root ganglia" Neuroscience Research. 15. 74-80 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1994-03-24  

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