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1993 Fiscal Year Final Research Report Summary

Study on UV-induced protease of hunam cells

Research Project

Project/Area Number 03680179
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionChiba University

Principal Investigator

SUZUKI Nobuo  Chiba University, Department of Biochemistry Assistant Professor, 医学部, 助教授 (90111426)

Project Period (FY) 1991 – 1993
KeywordsProtease / Human cells / Mutation / 突然変異 / 結節性硬化症
Research Abstract

In order to study the role of protease activity in tesponse of human cells to 254 nm for-ultraviolet light it will be necessary to purify the protease. Therefore, in the present study we attempted to establish cell strains with high levels of UV-induced protease activity. As the induction was observed in RSa cells pretreated with human interferon prior to UV and the protease activity was cells pretreated with human interferon prior to UV and the protease actibity was specifically ingibited by antipain, one of proteasw inhibitors, form RSa cells treated with low deses of antipain immediately after UV asurvival cells were cloned. The survival cells showed high levels protease activity after UV iffadiation and the induced protease activity was again inhibited by antipain. Inaddition to the vatiant cells already-established interferon-resistant cells also showed protease inducibility after UV.Both the variants appeared hypomutable in contrast to hypermuability of their parent RSa cells Furthermore, more increased DNA-repir capacity was observed in the variant cells inrradiated with UV than in the parent cells.
Thus, it was suggested that the protease activity play a role in error-free functions of human cells in early steps after UV.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Sugita,K.,Suzuki,N.,et al.: "In susceptibility of Cockyane syndrome-dorived lyaphocytes to plasminogen activator-like protease induction by ultra-violet rays and its abolition by interferon." Matation Res.262. 139-144 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.,et al.: "Dipyridamele enhances an anti-proliprative effect of interpron in various types of human tumor cells." Int.J.Cancer. 51. 627-633 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugita,K.,Suzuki,N.,et al.: "Serum facters responsible for unusual induction of plasminogen activator activity in tuberous sclerosis." J.Neurol.Sci.108. 202-206 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugita,K.,Suzuki,N.,et al.: "Involvement of cytokines in 15methyl-N′-ritro-N-nitrosoguanidine-induced plasminogen activator activity in acute disseninoted enceplurlo myolitis and multiple sclerosis lymphocytes." Eur.Neurol.33. 358-362 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,H.& Suzuki,N.: "Detection of K-ras codon 12 mutation by polymenoce chain reaction and differential dot-blot hybridization in sodium saccharin-frented human RSa cells." Biochem.Biophys.Res.Commun.196. 956-961 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,N.,et al.: "Human cell clones,RSa and UVK1,differing in their capaleility for UV-induced virus reactivation and phenotypic mutation." Mutation Res.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 鈴木信夫(分担): "動物培養細胞マニュアル" 共立出版株式会社, 368 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugita, K., Suzuki, N., et al.: "Insusceptibility of Cockayne syndrome-derived lymphocytes to pplasninogen activater-like protease induction by ultraviolet rays and its abolitio by interferon." Mutation Res.262. 139-144 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita, K., Suzuki, N., et al.: "Camparison of cellular sensitivity to UV killong with neurophysychological impairment in Cockayne syndrome patients." Brain Dev.13. 163-166 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N., et al.: "Dipyridamole enhances an anti-proliferative effect of interferon in various types of human tumor cells." Int. J.Cancer. 51. 627-633 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita, K., Suzuki, N., et al.: "Pelizaeus-Merzbacher Disease : Cellular hypersensitivity to ultraviolet light." Brain Dev.14. 44-47 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita, K., Suzuki, N., et al.: "Serum factors responsible for unusual induction of plasminogen activater activity in tuberous sclerosis." J.Neurol. Sci.108. 202-206 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugita, K., Suzuki, N., et al.: "Involvement of cytokines in N-Methyl-N'-Nitro-N-Nitrosoguanidine-induced plasminogen activator activity in acute disseminated encephalomyelitis and multiple sclerosis lymphocytes." Eur. Neurol.33. 358-362 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, H., and Suzuki, N.: "Detection of K-ras codon 12 mutation by polymerase chain reaction and differntial dot-blot hybridization in sodium saccharin-treated human RSa cell." Biochem. Biophy. Res.196. 956-961 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, N., et al.: "Human cell clones, RSa and UV-1, differing in their capability for UV-induced virus reactivation and phenotypic mutaion." Mutation Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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