1993 Fiscal Year Final Research Report Summary

MOLECULARBIOLOGY AND PHYSIOLOGY OF TASTE RECEPTION AND TRANSDUCTON USING DROSOPHILA TASTE GENE TRE.

Research Project

Project/Area Number	03680216
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	分子遺伝学・分子生理学
Research Institution	TOHOKU UNIVERSITY
Principal Investigator	ISONO Kunio TOHOKU UNIVERSITY GRADUATE SCHOOL OF INFORMATION SCIENCES, 大学院・情報科学研究科, 助手 (70124550)
Co-Investigator(Kenkyū-buntansha)	SIMADA Ichiro TOHOKU UNIVERSITY SCIENCES PROFESSOR, 理学部, 教授 (50108429)
Project Period (FY)	1991 – 1993
Keywords	DROSOPHILA / TASTE / MUTANT / MOLECULAR BIOLOGY / PHYSIOLOGY
Research Abstract	In vivo expression system using Xenopus oocyte by injecting polyA or total RNA extracted from labellar taste organs of the fleshfly has been established. An inward current was observed when the egg was perf used with sugar stimulants that stimulate fly taste receptors. In most cases the response was much higher for sugars like sucrose or glucose and smaller or absent for non-stimulating sugars like mannose or lactose. Genomic fragments that possibly cover the Drosophila taste gene Tre were isolated from YAC clones. Since the fragment contains more than 100 Kb length we are currently subcloning smaller fragments to identify the gene. The effect of Tre were analyzed physiologically by recording the labellar sensory neurons and compared with behavioral results that had been published earlier.