1992 Fiscal Year Final Research Report Summary
Analysis of ras genes mutations in human hepatic, biliary and pancreatic malignant tumors
| Project/Area Number |
03807041
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Chiba University |
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Principal Investigator |
ITO Yoshimi Chiba University Hospital assistant, 医学部附属病院, 助手 (70159929)
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| Co-Investigator(Kenkyū-buntansha) |
TADA Minoru Chiba University Hospital Resident, 医学部附属病院, 医員
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| Project Period (FY) |
1991 – 1992
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| Keywords | ras oncogene / point mutation / P C R / hepatocellular carcinoma / bile duct cancer / gallbladder cancer / pancreatic cancer |
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| Research Abstract |
The ras gene is one of the oncogenes most commonly detected in human cancers, and it consists of three families (H-ras, K-ras,N-ras). There genes are concerted to active oncogenes by point mutations occurring in either codon 12,13,or 61. We analyzed mutation of there codons in primary hepatic malignant tumors and panceatic cancers by a method to directly sequence nucleotides, using polymerase chain reaction and a direct sequencing method. There were no point mutations in any of hepatocellular carcinomas or hepatoblastoma around codon 12,13,or 61 ras genes. In contrast, there were point mutations in the all pancreatic cancers.
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