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1993 Fiscal Year Final Research Report Summary

Enzyme histochemical research of mononuclear cells and multinuclear-giant cells on the pressure sides in the periodontium incidental to experimental tooth movement

Research Project

Project/Area Number 03807125
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTsurumi University

Principal Investigator

HIRASHITA Ayao  Tsurumi University, School of Dental Medicine, Assosiate Professor, 歯学部, 助教授 (70089457)

Co-Investigator(Kenkyū-buntansha) KAI Tetsuya  Assistant, 歯学部, 助手 (80169375)
KAIDA Kiyokazu  Instructor, 歯学部, 講師 (50139606)
Project Period (FY) 1991 – 1993
Keywordsmacrophage / monocyte / osteoclast / preosteoclast / osteocalcin / osteoblast / non-specific esterase / tartrate-resistant acid phosphatase
Research Abstract

The purpose of this study was to elucidate the relationship between monocyte-macrophages and osteoclasts on the pressure sides in the periodontium incidental to experimental tooth movement. Enzymes of non-specific esterase (with or without NaF) were used for the identification of monocytes and macrophages, specific esterase was used for neutrophils and mast cells, tartrate-resistant acid phosphatase was used for preosteoclasts and mature osteoclasts.
Results : 1. Monocytes and macrophages were observed mainly near blood vessels, but most of cells were macrophages. 2. Many osteoclasts and a few preosteoclasts were seen on the surface of the alveolar bone and near the hyalinized tissues. 3. Neutrophils were seen only in the experiments of 1 and 12 hours'durations. 4. Mast cells were not observed in any of the experimental durations. 5. In bone marrow, many preosteoclasts and osteoclasts were observed. 6. The relationship between the appearances of macrophages and osteoclasts could not be correlated by an analysis of variance of the rations in the number of these cells after each periods.
These results suggest that there may be a fundamental biological difference between monocyte-macrophages and osteoclasts, and that some osteoclasts formed by fusion of preosteoclasts in bone marrow may migrate in the periodontium through the bone channel.
Now, experiments are in progress to study the effect of tooth movement on the changes in serum bone gla protein(BGP) which osteoblasts synthesize / secrete and osteoclasts release from the bone matrix by resorption.
A part of these results suggests that the decrease (days 3-5) and the increase (days 7-21) of serum BGP contents incidental to tooth movement may reflect bone turnover in the periodontium.
Further immunocytochemical study will be necessary for revealing of the intimate relationship between osteoblasts and osteoclasts incidental to tooth movement.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 平下斐雄: "変性組織と巨細胞-歯の移動時の圧迫歯根膜-" 歯界展望. 76. 399-412 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 甲斐哲也: "圧迫側歯根膜に出現するマクロファージと破骨細胞に関する酵素組織化学的研究" 日本矯正歯科学会雑誌. 50. 424-440 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平下斐雄: "骨系細胞の形態的特徴-in vivoによる-" 日本矯正歯科学会雑誌特別号. 51. 153-159 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ayao Hirashita: "Degenerated tissues and giant cells incidental to tooth movement" DENTAL OUTLOCK. Vol 76 No2. 399-412 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tetsuya Kai: "Enzyme histochemical research of macrophages and osteoclasts in pressure side incidental to experimental tooth movement" The Journal of Japan Orthodontic Society. Vol 50 No6. 424-440 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ayao Hirashita: "Morphological characteristics of bones' cells in vivo" The Journal of Japan Orthodontic Society. Vol 51 Special Issue. 153-159 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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