1992 Fiscal Year Final Research Report Summary
Platelet-activating factor in invertebrates
| Project/Area Number |
03808019
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | Teikyo University |
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Principal Investigator |
MABUCHI Kiyoko Teikyo University, Faculty of Pharmaceutical Sciences Research assistant, 薬学部, 助手 (30241277)
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| Project Period (FY) |
1991 – 1992
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| Keywords | Invertebrates / Phospholipids / Platelet-activating factor / Ether lipids / Acetyltransferase |
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| Research Abstract |
The levels of alkenylacyl, alkylacyl and diacyl subclasses of choline and ethanolamine glycerophospholipid fractions in 28 species of various invertebrtaes were examined. We found that only small amounts of either 1-alky-2-acyl-sn-glycero-3-phosphocholine or 1-alkenyl-2-acyl-sn-glycero-3-phosphoethanolamine are present in most species of insects. On the other hand, almost all species examined in various phyla other than Arthropoda were shown to contain large amounts of both alkylacyl-GPC and alkenylacyl-GPE. We next surveyed the presence of platelet-activating factor (PAF)-like lipid in 45 species of invertebrates. PAF-like lipid was widely distributed among various lower animals. The highest value was obtained for sea cucumber, in which PAF-like lipid was present throughout the body. We found that propionyl PAF is the major constituent of PAF-like lipid fraction at least in the case of earthworms. We also confirmed the presence of acetyltransferase activity in several lower animals. Earthworms contain strong acetyltransferase activity ; this enzyme activity is probably implicated in the synthesis of PAF at least in this animal. In contrast, sea cucumbers do not contain strong acetyltransferase activity. Sea cucumbers have strong enzyme activities for the de novo synthesis of PAF. These results suggest that the synthetic route of PAF is somewhat different among animal species.
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