1992 Fiscal Year Final Research Report Summary
Analysis of structure-function coupling in anion channel molecules
| Project/Area Number |
03808033
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Osaka University |
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Principal Investigator |
TAGUCHI Takahisa Osaka University, Faculty of Engineering Science, Department of Biophysical Engineering Assistant, 基礎工学部, 助手 (10197246)
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| Co-Investigator(Kenkyū-buntansha) |
TAGUCHI Takahisa Osaka University, Faculty of Engineering Science, Department of Biophysical Engi (10197246)
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| Project Period (FY) |
1991 – 1992
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| Keywords | channel protein / C1 channel / planar bilayer system |
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| Research Abstract |
A C1 channel in electric organs of Narke japonica was reconfirmed, using a polyclonal antibody, to be a 180k protein composed of two identical 90k units. The specific antibody against the 180k protein from electric organs reduced, when added in the cis side solution, the open probability of the C1 channel in a planar bilayer membrane without affecting the single channel conductance, ion selectivity nor voltage dependency. Furthermore, the antibody added in the trans side also affected the channel to increase the open probability. The eluate of the immuno-affinity chromatography was found to contain only the 180k protein. These results indicate the 180k protein to be an integral membrane protein and to form the C1 channel. To elucidate the biochemical atructure of the C1 channel in transverse tubules, we used the antibody. The western blot analysis with this antibody resulted that the antibody recognized 90k and 60k proteins, or only a 140k protein under the condition of electrophoresis with or without 2-mercaptoethanol, respectively.
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