1992 Fiscal Year Final Research Report Summary
Structure of the Myogenin Gene and Its Transcriptional Regulation
| Project/Area Number |
03833037
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子細胞生物学
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
FUJISAWA Atsuko Natl. Center of Neurol. and Psychiatry, Dept. of Mol.Genetics, Section Chief, 神経研究所, 室長 (60209038)
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| Co-Investigator(Kenkyū-buntansha) |
NABESHIMA Yo-ichi Natl. Center of Neurol. and Psychiatry, Dept. of Mol.Genetics, Director, 神経センター・神経研究所, 部長 (60108024)
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| Project Period (FY) |
1991 – 1992
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| Keywords | differentiation / myogenesis / myogenic regulatory factor / myogenin / C3H10T1 / 2 cell / transcriptional regulation / MyoD / developmental biology |
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| Research Abstract |
The recent identification of MyoD and related proteins, myogenin, MRF4/herculin/Myf-6, and Myf-5 has opened up new approaches to investigate molecular basis of muscle development. Non-muscle cells, such as embryonic fibroblasts, are converted to muscle cells after the expression of these myogenic proteins.
These factors belong to a family of regulatory proteins, designated as basic-helix-loop-helix proteins, which bind in vitro to the consensus sequence referred to as an E-box.
How individual members of these factors are involved in muscle development has not been clarified yet.
Studies on regulation of the myogenin gene in vitro and in vivo suggest that the role of the myogenin gene is crucial for muscle formation. It is expressed or activated in all the established myoblast cell lines so far examined. During embryogenesis, myogenin gene transcripts are initially detected in somites and in limb buds. Although expression of the myogenin gene dramatically decreases after birth, denervation
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reactivates its transcription. These studies suggest the involvement of myogenin not only in muscle cell terminal differentiation but also in the preceding myogenic processes. Thus, studies on regulatory mechanisms of the myogenin gene should illuminate molecular basis of myogenesis.
In an attempt to understand regulatory mechanisms which govern transcriptional activation of the myogenin gene, transgenic mice bearing the lacZ gene driven by the upstream region of the myogenin gene were generated. Stereoscopic visualization of LacZ-positive cells of these transtenic mouse embryos revealed that the upstream region of the myogenin gene conferred its transcriptional activation in cells of the skeletal muscle-lineages in somites, limb buds, and visceral arches. Moreover, transient LacZ expression in newly formed somites in addition to its strong activation in myotomal regions of mature somites with a rostro-caudal gradient raised the possibility that myogenin is transcriptionally activated in immature somites before myotome formation. Less
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[Publications] Fujisawa-Sehara,A., Nabeshima,Y., Komiya,T., Uetsuki,T., Asakura,A., and Nabeshima,Y: "Differential trans-activation of muscle-specific regulatory elements including the MLC box by chicken MyoD, myogenin, and MRF4" J.Biol.Chem. 267. 10031-10038 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Piette,J., Huchet,M., Duclert,A., Fujisawa-Sehara,A., and Changeux,J.-P: "Localization of mRNAs coding for CMD1, myogenin and the a-subunit of the acetylcholine receptor during skeletal muscle development in the chicken" Mechan. Dev. 37. 95-106 (1992)
Description
「研究成果報告書概要(欧文)」より
