1991 Fiscal Year Final Research Report Summary

ヒト・ゲノム解析研究

Research Project

Project/Area Number	03NP0401
Research Category	Grant-in-Aid for Creative Basic Research
Allocation Type	Single-year Grants
Research Institution	Osaka University
Principal Investigator	松原謙一大阪大学, 細胞工学センター, 教授 (20037394)
Co-Investigator(Kenkyū-buntansha)	大久保公策大阪大学, 細胞工学センター, 助手 (40233069) 中村祐輔 (財)癌研究会癌研究所, 部長 (70217909) 吉田光昭東京大学, 医科学研究所, 教授 (80012607) 吉川寛大阪大学, 医学部, 教授 (70019876) 榊佳之東京大学, 医科学研究所, 教授 (10112327)
Project Period (FY)	1991 – 1995
Keywords	ヒト・ゲノム / ゲノム構造解析 / ゲノム機能解析 / cDNA / DNA解析技術 / モデル生物 / 生物情報科学 / 物理的地図 / 遺伝的地図 / 塩基配列決定
Research Abstract	生命の設計図であるゲノムの構造と機能を研究解析し、そこに包含される遺伝情報を理解するために、総括班ならびに3つの研究班を組織して研究の推進を図り、次の成果を得た。 1.総括班。全体としての活動を円滑に進めるため各種会合の企画立案調整、機器の計画的購入と配分、情報交流、評価などを積極的に進めるほか、米国における新技術等の調査や各センタ-との打合わせ等により国際的に整合性のある我国のゲノム研究体制を確立した。また、社会の接点の問題研究の一環として国際生命倫理福井セミナ-を開き広く意見の交流と問題点の整理を図った。 2.ヒト・ゲノム構造解析班。ヒト染色体21番につき殆ど完全な形のNot I酒限酵素切断地図を作成したほか、6,8,X,Y,11につき約0.5〜2メガベ-スの密度でマ-カ-を置いてゆく物理的地図の作成作業を開始し、特定領域についてこれを完成させたほか、全体として約1/3〜1/2程度の完成度の地図作りを行った。これらの成果は、ゲノムデ-タベ-ス作成における1991年度分の国際的作業の約半分近くを占める貢献である。これと並んで、特定領域の詳細なマップ作りを進める作業を、免疫グロブリン遺伝子H鎖V領域とHLA遺伝子群について実行した。これは、大づかみなマップ作りが終わった後に来るべき仕事のモデルとなる先導的研究である。本年度のうちに、大量の資料を扱いつつそれらを処理、分析し、デ-タ化して整理する方法論の開発と実行において著しい進展を見せることができた。 3.cDNA解析班。ヒト肝細胞の中で発現している全遺伝子をカタログ化し、その発現頻度まで解析できる「3特異的cDNAライブラリ-分析」がスタ-トし、約2000クロ-ンの分析を終えるという成果を挙げた。この成功は、次年度から各臓器における発現遺伝子の系統的解析プロジェクトに発展できるものであり、世界に先駆けてゲノム研究に構造解析のみでなく機能解析をも並行して行うべきであるとする新しい方向性を与えるものである。これに伴って良質なcDNAライブラリ-の作成やその迅速な分類、カタログ化、デ-タ整理などにおける新しい手法が種々開発され、世界のcDNA研究者数の急速な増大を誘導するきっかけを作ったものと評価できる。 4.DNA解析技術開発班。DNAの分離、切断、クロ-ニング、染色体上へのマッピング等に関わる技術開発を進める中から、二次元電気泳動技術によるゲノムスキャンニング法の確立、T3ファ-ジによるDNA欠失セットの作成技術、BUDRを用いる染色体の分染とin situハイブリッド法の組み合わせ技術の確立という国際視献技術を完成することができた。また、枯草菌、分裂酵母、線虫をモデル生物として選び国内と国際協力してゲノムデ-タベ-スを作り且つそれに伴って高度の技術開発とデ-タベ-ス間の相互比較を導くシステム作りを現実化することができた。次年度から具体的成果の奔流が予想できる。

Research Products

(93 results)

All Other

All Publications (93 results)

[Publications] Saito,A.,Abad,J.S.,Wang,D.,Ohki,M.,Cantor,C.R.,and Smith,C.L.: "Construction and characterization of a NotI linking library of human chromoseme 21." Genomics. 10. 618-630 (1991)
[Publications] Shimizu,K.,Ichikawa,H.,Miyoshi,H.,Ohki,M.,Kobayashi,H.,Maseki,N.,and Kaneko,Y.: "Molecular assignment of a translocation breakpoint in acute myeloid liukemia with t(8;21)." Genes Chromosomes Cancer. 3. 163-167 (1991)
[Publications] Miyoshi,H.,Shimizu,K.,Kozu,T.,Maseki,N.,Kaneko,Y.,and Ohki,M.: "t(8;21) breakpoints' on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene,AML1." Proc.Natl.Acad.Sci.USA. 88. 10431-10434 (1991)
[Publications] Ichikawa,H.,Shimizu,K.,Saito,A.,Wang,D.,Oliva,R.,Kobayashi,H.,Kaneko,Y.,Miyoshi,H.,Smith,C.L.,Cantor,C.R.,and Ohki,M.: "Long-distance restriction mapping of the proximal long arm of human chromosome 21 with NotI linking clones." Proc.Nat1.Acad.Sci.USA. 89. 23-27 (1992)
[Publications] Morita,R.,Ishikawa,J.,Tsutsumi,M.,Hikiji,K.,Tsukada,Y.,Kamidono,S.,Maeda,S.,and Nakamura,Y.: "Allelotype of renal cell carcinoma." Cancer Res.51. 820-823 (1991)
[Publications] Morita,R.,Saito,S.,Ishikawa,J.,Ogawa,O.,Yoshida,O.,Yamakawa,K.,and Nakamura,Y.: "Common regions of deletion on chromosomes 5q,6q,and 10q in renal cell carcinoma." Cancer Res.51. 5817-5820 (1991)
[Publications] Sato,T.,Saito,H.,Morita,R.,Koi,S.,Lee,je H.and Nakamura,Y.: "Allelo type of human ovarian cancer." Cancer Res.51. 5188-5121 (1991)
[Publications] Saito,S.,Okui,K.,Tokino,T.,Oshimura,M.,and Nakamura,Y.: "Isolation and mapping of 68 RFLP markers on human chromosome 6." Am.J.Human Genet.50. 65-70 (1992)
[Publications] Nanko,S.,Kobayashi,M.,Gamou,S.,Kudoh,J.,Shimizu,N.,Takazawa,K.,Kazamatsuri,H.and Furusho,T.: "Linkage Analysis of Affective Disorder Using DNA Markers for Chromosomes 11 and X,Jpn." J.Psychiat.Neurol.45(1). 53-56 (1991)
[Publications] Shimizu,N;The Human Genome Project in Japan: "Current Status and Future Direction,In Genetics,Ethics and Human Values" Human Genome Mapping,Genetic Screening and Gene Therapy (eds) Bankowski,Z.and Capron,A.M.,CIOMS Pub.,Genova. 33-38 (1991)
[Publications] Araki,K.,Matsuo,N.,Kudoh,J.and Shimizu,N.: "RFLPs at the PAH(Phenylalanine Hydroxylase) Gene in the Japanese Population" Nucleic Acids Res.19(8). 1960 (1991)
[Publications] Minoshima,S.and Shimizu,N.: "Flow-Sorting of Human Chromosome 22 Derivatives for Physical Mapping and Fine Structure Analysis" In Flow Cytometry and Image Analysis for clinical Applications,(eds) Nishiya,I.,Cram,L.S.and Gray,J.W.Elsevier Science Publ.B.V.,Amsterdam. 59-63 (1991)
[Publications] Cox,D.R.and Shimizu,N.: "Report of the Committee on the Genetic Constitution of Chromosome 21 (HGM11),Cytogenet." Cell Genet.,. 58(1-2). 800-826 (1991)
[Publications] Kawasaki,K.,Minoshima,S.,Kudoh,J.,Fukuyama,R.and Shimizu,N.: "Methylation Status of Ribosomal RNA Gene Clusters in the Flow-Sorted Human Acrocentric Chromosomes,Mammalian Genome"
[Publications] Kawasaki,K.,Minoshima,S.,Kudoh,J.and Shimizu,N.: "Shotgun Polymerase Chain Reaction" Construction of Clone Libraries Specific to a NotI Fragment of Flow-Sorted Human Chromosome 22,Genomics.
[Publications] Hayashi,K.: ""PCR-SSCP:A Method for detection of mutations",Genetic Analysis:" Techniques and Applications.
[Publications] Nakagome,Y.,Nagafuchi,S.,Seki,S.,Nakagori,Y.,Tamura,T.,Yamada,M.,Iwaya,M.: "A repeating unit of the DYZ1 family on the human Y chromosome consists of segments with partial male specificity" Cytogenet.Cell Genet.56. 74-77 (1991)
[Publications] Shinohara,M.,Minowada,S.,Aso,Y.,Yamada,K.,Nakahori,Y.,Tamura,T.,Nakagome,Y.: "A t(Y;15) translocation with mixed gonadal dysgenesis" Hum.Genet.86. 442-444 (1991)
[Publications] Nagafuchi,S.,Seki,S.,Nakahori,Y.,Tamura,T.,Numabe,H.,Nakagome,Y.: PCR.
[Publications] Shin,E.K.,Matsuda,F.,Nagaoka,H.,Fukita,Y.,Imai,T.,Yokoyama,K.,Soeda,E.and Honjo,T.: "Physical map of the 3' region of the human immunoglobulin heavy chain locus:clustering of autoantibody-related variable segments in one haplotype" EMBO Journal. vol.10 no.12. 3641-3645 (1991)
[Publications] Kaneko,K.,Miyatake,T.,Migeon,B.,Warren,S.and Tsuji,S.: "Isolation of cosmid clones containing CpG islands at Xq24-Xq28 locus." Am.J.Hum.Genet.49. 381 (1991)
[Publications] Onodera,O.,Kobayashi,H.,Warren,S.T.,Miyatake,T.and Tsuji,S.: "Construction of human Xq24-qter hncDNA library highly enriched in human-derived hncDNA." Am.J.Hum.Genet.49. 444 (1991)
[Publications] Tabe,H.,Kaneko,K.,Kobayashi,H.,Miyatake,T.and Tsuji,S.: "Isolation of X chromosome specific cDNA clones expressed in human brain." Am.J.Hum.Genet.49. 445 (1991)
[Publications] Kaneko,K.,Wagner,M.J.,Wells,D.E.,Tanaka,H.,Miyatake,T.and Tsuji,S.: "A new RFLP locus D8S163 maps to human chromosome 8pter-8p22." Nucl.Acids Res.19. 6059 (1991)
[Publications] Suzuki,Y.,Sekiya,T.and Hayashi,K.: ""Allele-specific PCR:A method for amplification and sequence determination of a single component among a mixture of sequence variants" Anal." Biochem.192. 82-84 (1991)
[Publications] Murakami,Y.,Hayashi,K.and Sekiya,T.: ""Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single-stranded conformation polymorphism analysis of polymerase chain reaction products"" Cancer Res.51. 3356-3361 (1991)
[Publications] Hayashi,K.: ""PCR-SSCP:A simple and sensitive method for detection of mutations in the genomic DNA"" PCR:Methods and Applications. 1. 34-38 (1991)
[Publications] Iizuka,M.,Hayashi,K.and Sekiya,T.: ""Single-strand conformation polymorphism (SSCP) at the D8S86 locus"" nucleic Acids Res.19. 6346 (1991)
[Publications] Iizuka,M.,Mashiyama,S.,Oshimura,M.,Sekiya,T.and Hayashi,K.: ""A generic method of obtaining polymorphic sequence tagged sites:Polymerase chain reaction-single strand conformation polymorphism analysis of anonymous Alu repeats on chromosome 11"" Genomics. 12. 139-146 (1992)
[Publications] Morita,K.,J.Moriuchi,H.Inoko,K.Tsuji,and S.Arimori: "HLA Class II antigens and DNA restriction fragment length polymorphism in Myasthenia Gravis in Japan." Annals of Neurol. 29. 168-174 (1991)
[Publications] Inoue,D.,K.Sato,M.Maeda,H.Inoko,K.Tsuji,T.Mori,and H.Imura: "Genetic differences shown by HLA typing,among Japanese patients with euthyroid Graves' ophthalmopathy,Graves' disease and Hashimoto's thyroiditis:genetic characteristicsof euthyroid Graves' opthalmopathy." clinical Endocinol.34. 57-62 (1991)
[Publications] Sada.M.,H.Amemiya,and H.Inoko: "Analysisi of enzymatically amplitied HLA-DQA using restriction fragment length polymorphism." Transpl.Proc.23. 464-467 (1991)
[Publications] Ota.M.,T.Seki,N.Nomura,K.Sugimura,N.Mizuki,H.Fukushima,K.Tsuji,and H.Inoko: "Modified PCR-RFLP method for HLA-DPB1 and DQA1 genotyping." Tissue Antigens. 38. 60-71 (1991)
[Publications] Nomura.M.,M.Ota,K.Tsuji,and H.Inoko: "HLA-DQB1 genotyping by PCR-RFLP method combined with group-specific primers." Tissue Antigens. 38. 53-59 (1991)
[Publications] Okamoto.N.,A.Ando,J.Kawai,T.Yoshiwara,K.Tsuji,and H.Inoko: "Orientation of HLA-DNA gene and identification of a CpG island associated gene adjacent to DNA in human major histocompatibility complex class II region." Hum.Immunol.32. 221-228 (1991)
[Publications] Figulroa.F.,C.O.Uigin,H.Inoko,and J.Klein: "Primate DRB6 pseudogenes:clue to the evolutionary origin of the HLA-DR2 haplotype." Immunogenetics. 34:3. 324-337 (1991)
[Publications] Chifu,Y.,Nakashima,H.,and Imamura,T.: "β-Thalassemia major resulting from a compound heterozygosity for the β-globin gene mutation:further evidence for multiple origin and migration of the thalassemia gene." Hum Genet. 88. (1991)
[Publications] Muro,Y.,Masumoto,H.,Yoda,K.,Nozaki,N.,Ohashi,M.and Okazaki,T.: "Centromere protein B assembles human centromeric alpha-satellite DNA at the 17 bp sequence CENP-B box." J.Cell Biol.116. 585-596 (1992)
[Publications] Masumoto,H.,Masukata,H.,Muro,Y.,Nozaki,N.and Okazaki,T.: "A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA,a human centromeric satellite." J.Cell Biol.109. 1963-1973 (1989)
[Publications] Hirota,T.,Tsukamoto,K.,Deng,H-X.,Yoshiura,K.,Ohta,T.,Tohma,T.,Kibe,T.,Harada,N.,Jinno,Y.,and niikawa,N.: "Microdissection of human chromosomal regions 8q23.3-q24.11 and 2q33-qter:Construction of DNA libraries and isolation of their clones." Genomics. (1992)
[Publications] Deng,H-X.,Yoshiura,K.,Dirks,R.W.,Harada,N.,Hirota,T.,Tsukamoto,K.,Jinno,Y.,and Niikawa,N.: "Chromosome band-specific painting:Chromosome in situ suppression hybridization using PCR products from a microdissected chromosomal band as a probe pool." Hum.Genet.(1992)
[Publications] Jinno,Y.,Haraka,N.,Yoshiura,K.,Ohta,T.,Tohma,T.,Hirota,T.,Tsukamoto,K.,Deng,H-X.,Oshimura,M.,and Niikawa,N.: "A simple and efficient amplification method of DNA with unknown sequences and its application to the microdissection-microcloning." J.Biochem.(1992)
[Publications] Yoshioka,K.,Miki,T.,Katsuya,T.,Ogihara,T.,and Sasaki,Y.: "The 717 Vallle substitution in amyloid precursor protein is associated with familial Alzheimer's disease regardless of ethnic group." Biochm.Biophy.Res.Comm.178. 1141-1146 (1991)
[Publications] Kondo,I.,and Kanazawa,I.: "Association of Xbal allele (Xbal 44kb) of the human cytochrome P-450dbI (CYP2D6) gene in Japanese patients with idiopathic Parkinson's disease."Key Topics in Brain Research-Parkinson's Disease.From Clinical Aspects to Molecular Basis-"." T.Nagatsu,H.Narabayashi,and M.Yoshida (ed.),Springer-Verlag,New York. 111-117 (1991)
[Publications] Hakao,S.,Watanabe,M.,Yokoi,H.,Kogi,M.,Kondo,I.,Tsuchita H.,Kanazawa,I.,Wakasa,K.,Ikeda,J.: "Laser microdissection and single unique primer PCR allow generation of reginal chromosome DNA clones from a single human chromosome" Genomics. 11. 364-373 (1991)
[Publications] Sasaki,H.,Wakisaka,A.,Katoh,K.,Yoshida,M.C.,Hamada,T.,and Tashiro,K.: "Linkage study of dominantly inherited olivo-ponto-cerebellar atrophy (OPCA) and Holmes' ataxia." Jpn.J.Hum.Genet.33. 423-438 (1988)
[Publications] Sugano,S.Kim D.-W.,Yu,Y.-S.,Mizushima-Sugano,J.,Yoshitomo,K.,Watanabe,S.,Suzuki,F.,and Yamaguchi,N.,: "Use of an epitope-tagged cDNA library to isolate cDNAs of proteins with nuclear localization potential."
[Publications] Okubo,K.,N.Hori,R.Matoba,T.Niiyama,and K.Matsubara: "1991 A novel system for large-scal sequencing of cDNA by PCR amplification." DNA Sequence. 2. 137-144
[Publications] Hara,E.,Kato,T.,Nakada,S.,Sekiya,S.and Oda,K.: "Subtractive cDNA cloning using oligo(dT)_<30>-Latex and PCR:Isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells." nucl.Acids Res.19. 7097-7104 (1991)
[Publications] E.Takahashi,D.Ayusawa,S.Kaneda,Y.Itoh,T.Seno & T.Hori.: "The human ubiquitin-activating enzyme E1 gene (UBE1) mapped to band Xp11.23-p11.3 by fluorescence in situ hybridization." (1992) Cytogenet.Cell Genet.
[Publications] T.Hara,E.Takahashi,M.Yamauchi,M.Hoshino,K.Aoki,T.Hori,M.Kawakita & D.Ayusawa.: "The UDP-galactose translocator gene is mapped to the Wiskott-Aldrich syndrome locus of the X chromosome."
[Publications] Watanabe,S.,Nakayama,N.,Yokota,T.,Arai,K.and Miyajima,A.: "Colony stimulating factors and cytokine receptor network." Curr Opinion Biotechnol.2. 227-237 (1991)
[Publications] Miyajima,A.,Kitamura,T.,Haraka,N.,Yokota,T.and Arai,K.: "Cytokine receptors and signal transduction." Ann Rev Immunol. 10. 295-331 (1992)
[Publications] Nakayama,T.and Setoguchi,Y.: "Nucleotide sequence of a member of the chicken H2B histone-encoding gene family" Gene. 98. 299-300 (1991)
[Publications] Nakayama,T.: "Nucleotide sequences of two members of the chicken H3 histone-encoding gene family" Gene. 102. 289-290 (1991)
[Publications] Setoguchi,Y.and Nakayama,T.: "Nucleotide sequences of new members (H3-IV and H3-V) of the chicken H3 histone-encoding gene family" Nucleic Acids Res.19. 6327 (1991)
[Publications] Nakayama,T.,Takechi,S.,Ohshige,T.,Kondo,K.and Yamamoto,K.: "Nucleotide sequences of two members of the chicken H4 histoneencoding gene family" Gene. 108. 311-312 (1991)
[Publications] Seto,Y.,Fukunaga,R.,and Nagata,S.: "Chromosomal gene organization of the human granulocyte colony-stimulating factor receptor" J.Immunol.148. 259-266 (1992)
[Publications] Matsuzawa,Y.,Minagawa,K.,Yoshikawa,K.,and Doi,M.: ""Dynamic behavior of large DNA molecules in an aqueous solution toward the understanding of pulse-field electrophoresis,"" Nucleic Acids Symp.Ser.22. 77-78 (1990)
[Publications] Minagawa,K.,Matsuzawa,Y.,Yoshikawa,K.,Matsumoto,M.,and Doi,M.: ""Direct observation of the biphasic conformational change of DNA induced by cationic polymers,"" FEBS. 295. 67-69 (1991)
[Publications] Matsuzawa,Y.,Minagawa,K.,Yoshikawa,K.,Matsumoto,M.,and Doi,M.: ""Conformational dynamics of DNA affected by intercalation and minor groove binding:direct observation of large DNA."" Nucleic Acids Symp.Ser.25. 131-132 (1991)
[Publications] Matsumoto,M.,Sakaguchi,T.,Kimura,H.,Doi,M.,Minagawa,K.,Matsuzawa,Y.,and Yoshikawa,K.,: ""Direct observation of Brownian motion of macromolecules by fluorescence microscope,"" J.Polymer Sci.B.
[Publications] Shikata,T.,Kotaka,T.: "Biased sinusoidal field gel electrophoresis for size-dependent DNA separarion" Biopolymers. Vol.31. 253-254 (1991)
[Publications] Shikata,T.,Kotaka,T.: "Biased sinusoidal field gel electrophoresis for large DNA separation" Macromolecules. Vol.24. 4868-4873 (1991)
[Publications] Shimizu,M.,Konishi,A.,Shimada,Y.,Inoue,H.,and Ohtsuka,E.: "“Oligo(2^1-O-methyl)ribonucleotides:effective probes for duplex DNA""
[Publications] Uesawa,Y.and Sugiura,Y.: "“Heat-Induced DNA Cleavage by Esperamicin Antitumor Antibiotics"" Biochemistry. 30. 2989-2992 (1991)
[Publications] Hirama,M.,Gomibuchi,T.,Fujiwara,K.,Sugiura,Y.and Uesugi,M.: "“Synthesis and DNA-Cleaving Abilities of Functional Neocarzinostatin Chromophore Analogues"" J.Am.Chem.Soc.113. 9851-9853 (1991)
[Publications] Kimura,M.and Fujisawa,H.: "Dissection of functional domains of the packaging protein of bacteriophage T3 DNA by site-directed mutagenesis." Virology. 180. 709-715 (1991)
[Publications] Fujisawa,H.,Shibata,H.,and Kato,H.: "Analysis of interactions among factors involved in the bacteriophage T3 DNA packaging reaction in a defined in vitro system." Virology. 185. 788-794 (1991)
[Publications] Hashimoto,C.,and Fujisawa,H.: "DNA sequences necessary for packaging of bacteriophage T3 DNA." Virology. 187. (1992)
[Publications] Shin,E.K.,Matsuda,F.,Nagaoka,H.,Fukita,Y.,Imai,T.Yokoyama,K.,Soeda,E.and Honjo,T.: "Physical map of the 3^1 region of the human immunoglobulin heavy chain locus:clustering of autoantibody-related variable segments in one haplotype." EMBO J.10. 3641-3645 (1991)
[Publications] Tokino,T.,Imai,T.,Tanigami,A.,Takiguchi,S.and Nakamura,Y.: "Physical mapping of a 950-db region surrounding a locus (D10S102) tightly linked to the MEN2A gene." Genomics. 12. 394-400 (1992)
[Publications] Ito,W,Ishiguro,H.and Kurosawa,Y.: "A general method for introduction of a series of mutations in a cloned DNA using polymerase chain reaction." Gene. 102. 67-70 (1991)
[Publications] Matsuoka,T.,Kato,H.,Hashimoto,K.,Kurosawa,Y.: "Direct cloning of a long restriction fragment aided with a jumping clone." Gene. 107. 27-35 (1991)
[Publications] I.Hatada.,Y.Hayashizaki.,S.Hirotsune.,H.Komatsubara & T.Mukai: Proc.Natl.Acad.Sci.USA. 88. 9523-9527 (1991)
[Publications] Takahashi,E.,Hori,T.and Sutherland ,G.R.: "Mapping of the human type II collagen gene (COL2A1) proximal to fra(12)(q13.1) by nonisotopic in situ hybridization." Cytogenet.Cell Genet.54. 84-85 (1990)
[Publications] Tokino,T.,Takahashi,E.,Mori,M.,Tanigami,H.,Claser,T.,Park,J.W.,Jones,C.,Hori,T.and Nakamura,Y.: "Isolation and mapping of 62 new RFLP markers on human chromosome 11." Am.J.Hum.Genet.48. 258-268 (1991)
[Publications] Yamakawa,K.,Takahashi,E.,Saito,H.,Sato,T.,Oshimura,M.,Hori,T.and Nakamura,Y.: "Isolation and mapping of 75 new DNA markers on human chromosome 3" Genomics. 9. 536-543 (1991)
[Publications] Takahashi,E.,Yamakawa,K.,Nakamura,Y.and Hori,T.: "A high-resolution cytogenetic map of human chromosome 3:localization of 366 new cosmid markers (including 142 RFLPs) by a direct mapping system with fluorescence in situ hybridization." (1992)
[Publications] Ozawa,K.,Sudo,T.,Soeda,E.,Yoshida,M.C.and Ishii,S.: "Assignment of the human CREB2(CRE-BP1) gene to 2q32." Genomics. 10. 1103-1104 (1991)
[Publications] Arinami,T.,Ishikawa,M.,Inoude,A.,Yanagisawa,M.,Masaki,T.,Yoshida,M.C.and Hamaguchi,H.: "Chromosomal assignments of the human endothelin family genes:The endothelin-1 gene (EDN1) to 6p23-p24,the endothelin-2 (EDN2) to 1p34,and the endothelin-3 gene (EDN3) to 20q13.2-q13.3." Am.J.Hum.Genet.48. 990-996 (1991)
[Publications] Noda,A.,Courtright,J.B.,Denor,P.F.,Webb,G.,Kohara,Y.and Ishihama,A.: "Rapid Identification of speeific genes in !E.coli by hybridization to membranes containing the ordered set of phage clones." Bio Techniques. 10. 474-479 (1991)
[Publications] Itaya,M.and Tanaka,T.: "Complete physical map of the Bacillus subtilis 168 chromosome constructed by a gene-directed mutagenesis method." J.Mol.Biol.220. 631-648 (1991)
[Publications] Ogasawara,N.,and Kobayashi,Y.: "Genes and proteins for cell growth and division in Bacillus subtilis." In Control of Cell Growth and Division (ed.by Ishihama,A.and Yoshikawa,H.),Japan Sci.Soc.Press,Tokyo/Springer-Verlag,Berlin. 225-234 (1991)
[Publications] Sadaie,Y.,Takamatsu,H.,Nakamura,K.,and Yamane,K.: "Sequencing reveals similarity of the wild-type div^+ gene of Bacillus subtilis to the Escherichia coli secA gene." Gene. 98. 101-105 (1991)
[Publications] Yamamoto,J.,Shimizu,M.,and Yamane,K.: "Molecular cloning and analysis of nucleotide sequence of the Bacillus subtilis lysA gene region using B.subtilis phage vectors and a multicopy plasmid,pUB110." Agric.Biol.Chem.55. 1615-1626 (1991)
[Publications] Fujita,Y.,Shindo,K.,Miwa,Y.,and Yoshida,K.: "Bacillus subtilis inositol degydrogenase gene (idh):sequence and expression in Escherichia coli." Gene. 108. 121-125 (1991)
[Publications] Yoshikawa,H.and Ogasawara,N.: "Structure and function of DnaA and the DnaA-box in eubacteria:evolutionary relationships of bacterial replication origins." Mol.Microbiol.5. 2589 (1991)
[Publications] Ogawawara,N.and Yoshikawa,H.: "Genes and their organization in the replication origin region of the bacterial chromosome." Mol.Microbiol.6. (1982)
[Publications] Miyao,A.,Yoshimura,A.,Sato,T.,Yamamoto,T.,Theeragool,G.,and Kobayashi,Y.: "Sequence of the Bacillus subtilis homolog of Escherichia coli cell division gene murG." Gene. (1992)
[Publications] T.Ito,H.Ohgusu,N.Oishi and Y.Sakaki: "An improved pulsed-field polyacrylamide gel electrophoresis system for physical selection of linking clones:Isolation of SfiI linkin clones from a chromosome 21-specific library." Genomics. 9. 707-712 (1991)
[Publications] H.Sasaki,T.Hamada,T.Ueda,R.Seki,T.Higashinakagawa and Y.Sakaki: "Inherited type of allelic methylation variations in a mouse chromosome region where and integrated transgene shows methylation imprinting." Development. 111. 573-581 (1991)
[Publications] K.Yoshioka,N.Oishi and Y.Sakaki: "A polymorphism in the human APP gene detected by SSCP." Hum.Mol.Genet.(1992)

1991 Fiscal Year Final Research Report Summary

ヒト・ゲノム解析研究

Principal Investigator

松原 謙一 大阪大学, 細胞工学センター, 教授 (20037394)

Research Products

[Publications] Saito,A.,Abad,J.S.,Wang,D.,Ohki,M.,Cantor,C.R.,and Smith,C.L.: "Construction and characterization of a NotI linking library of human chromoseme 21." Genomics. 10. 618-630 (1991)

[Publications] Shimizu,K.,Ichikawa,H.,Miyoshi,H.,Ohki,M.,Kobayashi,H.,Maseki,N.,and Kaneko,Y.: "Molecular assignment of a translocation breakpoint in acute myeloid liukemia with t(8;21)." Genes Chromosomes Cancer. 3. 163-167 (1991)

[Publications] Miyoshi,H.,Shimizu,K.,Kozu,T.,Maseki,N.,Kaneko,Y.,and Ohki,M.: "t(8;21) breakpoints' on chromosome 21 in acute myeloid leukemia are clustered within a limited region of a single gene,AML1." Proc.Natl.Acad.Sci.USA. 88. 10431-10434 (1991)

[Publications] Ichikawa,H.,Shimizu,K.,Saito,A.,Wang,D.,Oliva,R.,Kobayashi,H.,Kaneko,Y.,Miyoshi,H.,Smith,C.L.,Cantor,C.R.,and Ohki,M.: "Long-distance restriction mapping of the proximal long arm of human chromosome 21 with NotI linking clones." Proc.Nat1.Acad.Sci.USA. 89. 23-27 (1992)

[Publications] Morita,R.,Ishikawa,J.,Tsutsumi,M.,Hikiji,K.,Tsukada,Y.,Kamidono,S.,Maeda,S.,and Nakamura,Y.: "Allelotype of renal cell carcinoma." Cancer Res.51. 820-823 (1991)

[Publications] Morita,R.,Saito,S.,Ishikawa,J.,Ogawa,O.,Yoshida,O.,Yamakawa,K.,and Nakamura,Y.: "Common regions of deletion on chromosomes 5q,6q,and 10q in renal cell carcinoma." Cancer Res.51. 5817-5820 (1991)

[Publications] Sato,T.,Saito,H.,Morita,R.,Koi,S.,Lee,je H.and Nakamura,Y.: "Allelo type of human ovarian cancer." Cancer Res.51. 5188-5121 (1991)

[Publications] Saito,S.,Okui,K.,Tokino,T.,Oshimura,M.,and Nakamura,Y.: "Isolation and mapping of 68 RFLP markers on human chromosome 6." Am.J.Human Genet.50. 65-70 (1992)

[Publications] Nanko,S.,Kobayashi,M.,Gamou,S.,Kudoh,J.,Shimizu,N.,Takazawa,K.,Kazamatsuri,H.and Furusho,T.: "Linkage Analysis of Affective Disorder Using DNA Markers for Chromosomes 11 and X,Jpn." J.Psychiat.Neurol.45(1). 53-56 (1991)

[Publications] Shimizu,N;The Human Genome Project in Japan: "Current Status and Future Direction,In Genetics,Ethics and Human Values" Human Genome Mapping,Genetic Screening and Gene Therapy (eds) Bankowski,Z.and Capron,A.M.,CIOMS Pub.,Genova. 33-38 (1991)

[Publications] Araki,K.,Matsuo,N.,Kudoh,J.and Shimizu,N.: "RFLPs at the PAH(Phenylalanine Hydroxylase) Gene in the Japanese Population" Nucleic Acids Res.19(8). 1960 (1991)

[Publications] Minoshima,S.and Shimizu,N.: "Flow-Sorting of Human Chromosome 22 Derivatives for Physical Mapping and Fine Structure Analysis" In Flow Cytometry and Image Analysis for clinical Applications,(eds) Nishiya,I.,Cram,L.S.and Gray,J.W.Elsevier Science Publ.B.V.,Amsterdam. 59-63 (1991)

[Publications] Cox,D.R.and Shimizu,N.: "Report of the Committee on the Genetic Constitution of Chromosome 21 (HGM11),Cytogenet." Cell Genet.,. 58(1-2). 800-826 (1991)

[Publications] Kawasaki,K.,Minoshima,S.,Kudoh,J.,Fukuyama,R.and Shimizu,N.: "Methylation Status of Ribosomal RNA Gene Clusters in the Flow-Sorted Human Acrocentric Chromosomes,Mammalian Genome"

[Publications] Kawasaki,K.,Minoshima,S.,Kudoh,J.and Shimizu,N.: "Shotgun Polymerase Chain Reaction" Construction of Clone Libraries Specific to a NotI Fragment of Flow-Sorted Human Chromosome 22,Genomics.

[Publications] Hayashi,K.: ""PCR-SSCP:A Method for detection of mutations",Genetic Analysis:" Techniques and Applications.

[Publications] Nakagome,Y.,Nagafuchi,S.,Seki,S.,Nakagori,Y.,Tamura,T.,Yamada,M.,Iwaya,M.: "A repeating unit of the DYZ1 family on the human Y chromosome consists of segments with partial male specificity" Cytogenet.Cell Genet.56. 74-77 (1991)

[Publications] Shinohara,M.,Minowada,S.,Aso,Y.,Yamada,K.,Nakahori,Y.,Tamura,T.,Nakagome,Y.: "A t(Y;15) translocation with mixed gonadal dysgenesis" Hum.Genet.86. 442-444 (1991)

[Publications] Nagafuchi,S.,Seki,S.,Nakahori,Y.,Tamura,T.,Numabe,H.,Nakagome,Y.: PCR.

[Publications] Shin,E.K.,Matsuda,F.,Nagaoka,H.,Fukita,Y.,Imai,T.,Yokoyama,K.,Soeda,E.and Honjo,T.: "Physical map of the 3' region of the human immunoglobulin heavy chain locus:clustering of autoantibody-related variable segments in one haplotype" EMBO Journal. vol.10 no.12. 3641-3645 (1991)

[Publications] Kaneko,K.,Miyatake,T.,Migeon,B.,Warren,S.and Tsuji,S.: "Isolation of cosmid clones containing CpG islands at Xq24-Xq28 locus." Am.J.Hum.Genet.49. 381 (1991)

[Publications] Onodera,O.,Kobayashi,H.,Warren,S.T.,Miyatake,T.and Tsuji,S.: "Construction of human Xq24-qter hncDNA library highly enriched in human-derived hncDNA." Am.J.Hum.Genet.49. 444 (1991)

[Publications] Tabe,H.,Kaneko,K.,Kobayashi,H.,Miyatake,T.and Tsuji,S.: "Isolation of X chromosome specific cDNA clones expressed in human brain." Am.J.Hum.Genet.49. 445 (1991)

[Publications] Kaneko,K.,Wagner,M.J.,Wells,D.E.,Tanaka,H.,Miyatake,T.and Tsuji,S.: "A new RFLP locus D8S163 maps to human chromosome 8pter-8p22." Nucl.Acids Res.19. 6059 (1991)

[Publications] Suzuki,Y.,Sekiya,T.and Hayashi,K.: ""Allele-specific PCR:A method for amplification and sequence determination of a single component among a mixture of sequence variants" Anal." Biochem.192. 82-84 (1991)

[Publications] Murakami,Y.,Hayashi,K.and Sekiya,T.: ""Detection of aberrations of the p53 alleles and the gene transcript in human tumor cell lines by single-stranded conformation polymorphism analysis of polymerase chain reaction products"" Cancer Res.51. 3356-3361 (1991)

[Publications] Hayashi,K.: ""PCR-SSCP:A simple and sensitive method for detection of mutations in the genomic DNA"" PCR:Methods and Applications. 1. 34-38 (1991)

[Publications] Iizuka,M.,Hayashi,K.and Sekiya,T.: ""Single-strand conformation polymorphism (SSCP) at the D8S86 locus"" nucleic Acids Res.19. 6346 (1991)

[Publications] Iizuka,M.,Mashiyama,S.,Oshimura,M.,Sekiya,T.and Hayashi,K.: ""A generic method of obtaining polymorphic sequence tagged sites:Polymerase chain reaction-single strand conformation polymorphism analysis of anonymous Alu repeats on chromosome 11"" Genomics. 12. 139-146 (1992)

[Publications] Morita,K.,J.Moriuchi,H.Inoko,K.Tsuji,and S.Arimori: "HLA Class II antigens and DNA restriction fragment length polymorphism in Myasthenia Gravis in Japan." Annals of Neurol. 29. 168-174 (1991)

[Publications] Sada.M.,H.Amemiya,and H.Inoko: "Analysisi of enzymatically amplitied HLA-DQA using restriction fragment length polymorphism." Transpl.Proc.23. 464-467 (1991)

[Publications] Ota.M.,T.Seki,N.Nomura,K.Sugimura,N.Mizuki,H.Fukushima,K.Tsuji,and H.Inoko: "Modified PCR-RFLP method for HLA-DPB1 and DQA1 genotyping." Tissue Antigens. 38. 60-71 (1991)

[Publications] Nomura.M.,M.Ota,K.Tsuji,and H.Inoko: "HLA-DQB1 genotyping by PCR-RFLP method combined with group-specific primers." Tissue Antigens. 38. 53-59 (1991)

[Publications] Okamoto.N.,A.Ando,J.Kawai,T.Yoshiwara,K.Tsuji,and H.Inoko: "Orientation of HLA-DNA gene and identification of a CpG island associated gene adjacent to DNA in human major histocompatibility complex class II region." Hum.Immunol.32. 221-228 (1991)

[Publications] Figulroa.F.,C.O.Uigin,H.Inoko,and J.Klein: "Primate DRB6 pseudogenes:clue to the evolutionary origin of the HLA-DR2 haplotype." Immunogenetics. 34:3. 324-337 (1991)

[Publications] Chifu,Y.,Nakashima,H.,and Imamura,T.: "β-Thalassemia major resulting from a compound heterozygosity for the β-globin gene mutation:further evidence for multiple origin and migration of the thalassemia gene." Hum Genet. 88. (1991)

[Publications] Muro,Y.,Masumoto,H.,Yoda,K.,Nozaki,N.,Ohashi,M.and Okazaki,T.: "Centromere protein B assembles human centromeric alpha-satellite DNA at the 17 bp sequence CENP-B box." J.Cell Biol.116. 585-596 (1992)

[Publications] Masumoto,H.,Masukata,H.,Muro,Y.,Nozaki,N.and Okazaki,T.: "A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA,a human centromeric satellite." J.Cell Biol.109. 1963-1973 (1989)

[Publications] Hirota,T.,Tsukamoto,K.,Deng,H-X.,Yoshiura,K.,Ohta,T.,Tohma,T.,Kibe,T.,Harada,N.,Jinno,Y.,and niikawa,N.: "Microdissection of human chromosomal regions 8q23.3-q24.11 and 2q33-qter:Construction of DNA libraries and isolation of their clones." Genomics. (1992)

[Publications] Deng,H-X.,Yoshiura,K.,Dirks,R.W.,Harada,N.,Hirota,T.,Tsukamoto,K.,Jinno,Y.,and Niikawa,N.: "Chromosome band-specific painting:Chromosome in situ suppression hybridization using PCR products from a microdissected chromosomal band as a probe pool." Hum.Genet.(1992)

[Publications] Jinno,Y.,Haraka,N.,Yoshiura,K.,Ohta,T.,Tohma,T.,Hirota,T.,Tsukamoto,K.,Deng,H-X.,Oshimura,M.,and Niikawa,N.: "A simple and efficient amplification method of DNA with unknown sequences and its application to the microdissection-microcloning." J.Biochem.(1992)

[Publications] Yoshioka,K.,Miki,T.,Katsuya,T.,Ogihara,T.,and Sasaki,Y.: "The 717 Vallle substitution in amyloid precursor protein is associated with familial Alzheimer's disease regardless of ethnic group." Biochm.Biophy.Res.Comm.178. 1141-1146 (1991)

[Publications] Hakao,S.,Watanabe,M.,Yokoi,H.,Kogi,M.,Kondo,I.,Tsuchita H.,Kanazawa,I.,Wakasa,K.,Ikeda,J.: "Laser microdissection and single unique primer PCR allow generation of reginal chromosome DNA clones from a single human chromosome" Genomics. 11. 364-373 (1991)

[Publications] Sasaki,H.,Wakisaka,A.,Katoh,K.,Yoshida,M.C.,Hamada,T.,and Tashiro,K.: "Linkage study of dominantly inherited olivo-ponto-cerebellar atrophy (OPCA) and Holmes' ataxia." Jpn.J.Hum.Genet.33. 423-438 (1988)

[Publications] Sugano,S.Kim D.-W.,Yu,Y.-S.,Mizushima-Sugano,J.,Yoshitomo,K.,Watanabe,S.,Suzuki,F.,and Yamaguchi,N.,: "Use of an epitope-tagged cDNA library to isolate cDNAs of proteins with nuclear localization potential."

[Publications] Okubo,K.,N.Hori,R.Matoba,T.Niiyama,and K.Matsubara: "1991 A novel system for large-scal sequencing of cDNA by PCR amplification." DNA Sequence. 2. 137-144

[Publications] Hara,E.,Kato,T.,Nakada,S.,Sekiya,S.and Oda,K.: "Subtractive cDNA cloning using oligo(dT)_<30>-Latex and PCR:Isolation of cDNA clones specific to undifferentiated human embryonal carcinoma cells." nucl.Acids Res.19. 7097-7104 (1991)

[Publications] E.Takahashi,D.Ayusawa,S.Kaneda,Y.Itoh,T.Seno & T.Hori.: "The human ubiquitin-activating enzyme E1 gene (UBE1) mapped to band Xp11.23-p11.3 by fluorescence in situ hybridization." (1992) Cytogenet.Cell Genet.

[Publications] T.Hara,E.Takahashi,M.Yamauchi,M.Hoshino,K.Aoki,T.Hori,M.Kawakita & D.Ayusawa.: "The UDP-galactose translocator gene is mapped to the Wiskott-Aldrich syndrome locus of the X chromosome."

[Publications] Watanabe,S.,Nakayama,N.,Yokota,T.,Arai,K.and Miyajima,A.: "Colony stimulating factors and cytokine receptor network." Curr Opinion Biotechnol.2. 227-237 (1991)

[Publications] Miyajima,A.,Kitamura,T.,Haraka,N.,Yokota,T.and Arai,K.: "Cytokine receptors and signal transduction." Ann Rev Immunol. 10. 295-331 (1992)

[Publications] Nakayama,T.and Setoguchi,Y.: "Nucleotide sequence of a member of the chicken H2B histone-encoding gene family" Gene. 98. 299-300 (1991)

[Publications] Nakayama,T.: "Nucleotide sequences of two members of the chicken H3 histone-encoding gene family" Gene. 102. 289-290 (1991)

[Publications] Setoguchi,Y.and Nakayama,T.: "Nucleotide sequences of new members (H3-IV and H3-V) of the chicken H3 histone-encoding gene family" Nucleic Acids Res.19. 6327 (1991)

[Publications] Nakayama,T.,Takechi,S.,Ohshige,T.,Kondo,K.and Yamamoto,K.: "Nucleotide sequences of two members of the chicken H4 histoneencoding gene family" Gene. 108. 311-312 (1991)

[Publications] Seto,Y.,Fukunaga,R.,and Nagata,S.: "Chromosomal gene organization of the human granulocyte colony-stimulating factor receptor" J.Immunol.148. 259-266 (1992)

[Publications] Matsuzawa,Y.,Minagawa,K.,Yoshikawa,K.,and Doi,M.: ""Dynamic behavior of large DNA molecules in an aqueous solution toward the understanding of pulse-field electrophoresis,"" Nucleic Acids Symp.Ser.22. 77-78 (1990)

[Publications] Minagawa,K.,Matsuzawa,Y.,Yoshikawa,K.,Matsumoto,M.,and Doi,M.: ""Direct observation of the biphasic conformational change of DNA induced by cationic polymers,"" FEBS. 295. 67-69 (1991)

[Publications] Matsuzawa,Y.,Minagawa,K.,Yoshikawa,K.,Matsumoto,M.,and Doi,M.: ""Conformational dynamics of DNA affected by intercalation and minor groove binding:direct observation of large DNA."" Nucleic Acids Symp.Ser.25. 131-132 (1991)

[Publications] Matsumoto,M.,Sakaguchi,T.,Kimura,H.,Doi,M.,Minagawa,K.,Matsuzawa,Y.,and Yoshikawa,K.,: ""Direct observation of Brownian motion of macromolecules by fluorescence microscope,"" J.Polymer Sci.B.

[Publications] Shikata,T.,Kotaka,T.: "Biased sinusoidal field gel electrophoresis for size-dependent DNA separarion" Biopolymers. Vol.31. 253-254 (1991)

[Publications] Shikata,T.,Kotaka,T.: "Biased sinusoidal field gel electrophoresis for large DNA separation" Macromolecules. Vol.24. 4868-4873 (1991)

[Publications] Shimizu,M.,Konishi,A.,Shimada,Y.,Inoue,H.,and Ohtsuka,E.: "“Oligo(2^1-O-methyl)ribonucleotides:effective probes for duplex DNA""

[Publications] Uesawa,Y.and Sugiura,Y.: "“Heat-Induced DNA Cleavage by Esperamicin Antitumor Antibiotics"" Biochemistry. 30. 2989-2992 (1991)

[Publications] Hirama,M.,Gomibuchi,T.,Fujiwara,K.,Sugiura,Y.and Uesugi,M.: "“Synthesis and DNA-Cleaving Abilities of Functional Neocarzinostatin Chromophore Analogues"" J.Am.Chem.Soc.113. 9851-9853 (1991)

[Publications] Kimura,M.and Fujisawa,H.: "Dissection of functional domains of the packaging protein of bacteriophage T3 DNA by site-directed mutagenesis." Virology. 180. 709-715 (1991)

[Publications] Fujisawa,H.,Shibata,H.,and Kato,H.: "Analysis of interactions among factors involved in the bacteriophage T3 DNA packaging reaction in a defined in vitro system." Virology. 185. 788-794 (1991)

[Publications] Hashimoto,C.,and Fujisawa,H.: "DNA sequences necessary for packaging of bacteriophage T3 DNA." Virology. 187. (1992)

[Publications] Shin,E.K.,Matsuda,F.,Nagaoka,H.,Fukita,Y.,Imai,T.Yokoyama,K.,Soeda,E.and Honjo,T.: "Physical map of the 3^1 region of the human immunoglobulin heavy chain locus:clustering of autoantibody-related variable segments in one haplotype." EMBO J.10. 3641-3645 (1991)

[Publications] Tokino,T.,Imai,T.,Tanigami,A.,Takiguchi,S.and Nakamura,Y.: "Physical mapping of a 950-db region surrounding a locus (D10S102) tightly linked to the MEN2A gene." Genomics. 12. 394-400 (1992)

[Publications] Ito,W,Ishiguro,H.and Kurosawa,Y.: "A general method for introduction of a series of mutations in a cloned DNA using polymerase chain reaction." Gene. 102. 67-70 (1991)

[Publications] Matsuoka,T.,Kato,H.,Hashimoto,K.,Kurosawa,Y.: "Direct cloning of a long restriction fragment aided with a jumping clone." Gene. 107. 27-35 (1991)

[Publications] I.Hatada.,Y.Hayashizaki.,S.Hirotsune.,H.Komatsubara & T.Mukai: Proc.Natl.Acad.Sci.USA. 88. 9523-9527 (1991)

[Publications] Takahashi,E.,Hori,T.and Sutherland ,G.R.: "Mapping of the human type II collagen gene (COL2A1) proximal to fra(12)(q13.1) by nonisotopic in situ hybridization." Cytogenet.Cell Genet.54. 84-85 (1990)

[Publications] Tokino,T.,Takahashi,E.,Mori,M.,Tanigami,H.,Claser,T.,Park,J.W.,Jones,C.,Hori,T.and Nakamura,Y.: "Isolation and mapping of 62 new RFLP markers on human chromosome 11." Am.J.Hum.Genet.48. 258-268 (1991)

松原謙一大阪大学, 細胞工学センター, 教授 (20037394)