1993 Fiscal Year Final Research Report Summary
Reaction of alpha2-macroglobulin Homologs in Self Defense System
| Project/Area Number |
04044061
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
IKAI Atsushi Tokyo Institute of Technology, Professor, 生命理工学部, 教授 (50011713)
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| Co-Investigator(Kenkyū-buntansha) |
OSTERBERG Ragnar Swedish University of Agricultural Sciences, 化学科, 教授
ARMSTRONG Peler B University of California at Davis, デービス校・動物学科, 教授
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| Project Period (FY) |
1992 – 1993
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| Keywords | Alpha-2-macroglobulin / Bait region / X-ray solution scattering / Thiolester bonds / Quaternary stucture change |
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| Research Abstract |
The reaction mechanism and the biological activities of the unique proteinase inhibitor alpha-2-macroglobulin were studied in collaboration with Professors Peter Armstrong and Ragnar Osterberg. The rates of bait region cleavage, thiolester cleavage, and that of structural change were determined by using specific probes for each step for the first time since the discovery of this protein in 1960's. The intramolecular events triggered by the bait region cleavage reaction can now be described in clearer molecular terms than before. The biological use of this unique mechanism as the carrier of weakly immunogenic peptide vaccines was clearly demonstrated and being further developed to include the neurite extension activity of this protein.
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Research Products
(20 results)
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[Publications] Mitsuda,S.,Nakagawa,T.,Osada,T.,Shimamoto,T.,Nakazato,H.and Ikai,A.: "A Receptor Mediated Delivery of HIV-1 Derived Peptide Vaccine" Biochem.Biophys.Res.Commun.(in press).
Description
「研究成果報告書概要(和文)」より
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[Publications] Athauda,S.B.P.,Arakawa,H.,Nishigai,M.,Takahashi,T.,Ikai,A.and Takahashi,K.: "Inhibition of Cathepsin E by α_2-Macroglobulin and the Resulting Structural Changes in the Inhibitor" J Biochem.113. 526-530 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Athauda,S.B.P.,Ido,E.,Arakawa,H.,Nishigai,M.,Kyushiki,H.,Yoshinaka,Y.,Takahashi,T.,Ikai,A.,Tang,J.and Takahashi,K.: "Entrapment and Inhibition of Human Immunodeficiency Virus Proteinase by α_2-Macroglobulin and Structural" J.Biochem.113. 742-746 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Arakawa,H.,Urisaka,T.,Tsuruta,H.,Amemiya,Y.,Kihara,H.and Ikai,A.: "The kinetics of conformational changes of α_2-macroglobulin determined by time resolved..." FEBS Letters. 337. 171-174 (1994)
Description
「研究成果報告書概要(和文)」より
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[Publications] Mitsuda,S., Nakagawa,T., Osada,T., Shimamoto,T., Nakazato,H. and Ikai,A.: "A Receptor Mediated Delivery of HIV-1 Derived Peptide Vaccine" Biochem.Biophys.Res.Commun.(in press).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Athauda,S.B.P., Arakawa,H., Nishigai,M., Takahashi,T., Ikai,A. and Takahashi,K.: "Inhibition of Cathepsin E by alpha_2-Macroglobulin and the Resulting Structural Changes in the Inhibitor" J.Biochem.113. 526-530 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Athauda,S.B.P., Ido,E., Arakawa,H., Nishigai,M., Kyushiki,H., Yoshinaka,Y., Takahashi,T., Ikai,A., Tang,J. and Takahashi, K.: "Entrapment and Inhibition of Human Immunodeficiency Virus Proteinase by alpha_2-Macroglobulin---" J.Biochem.113. 742-746 (1993)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Arakawa,H., Urisaka,T., Tsuruta,H., Amemiya,Y., Kihara,H. and Ikai,A.: "The kinetics of conformational changes of alpha_2-macroglobulin determined by time resolved X-ray" FEBS Letters. 337. 171-174 (1994)
Description
「研究成果報告書概要(欧文)」より
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