1993 Fiscal Year Final Research Report Summary
Study on recombination signal binding protein ; RBP-Jkappa
| Project/Area Number |
04044094
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Kyoto University, Faculty of Medicine |
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Principal Investigator |
NAKANO Toru Kyoto University, Faculty of Medicine, Lectureer, 医学部, 講師 (00172370)
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| Co-Investigator(Kenkyū-buntansha) |
TSUBATA Takeshi Kyoto University, Faculty of Medicine, 医学部, 助教授 (80197756)
ASHBURNER Michael Cambridge University
MAK Tak W. Ontario Cancer Institute, Toronto University
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| Project Period (FY) |
1992 – 1993
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| Keywords | Gene targeting / Homologous recombination / Embryonic lethal / Development / Transcriptional regulator |
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| Research Abstract |
The Jkappa-RBP gene was cloned as the gene which encodes the protein binding to the immunoglobulin recombination signal sequence flanking the kappa-type J segment (Jkappa-RS). However, expression of the gene is not restricted to the lymphoid system. And no functions of mouse Jkappa-RBP other than binding to Jkappa-RS have been found. Meanwhile, the Drosophila homologue of Jkappa-RBP gene turned out to be the gene known as Suppressor of Hairless (Sup(H)) whose mutation affects peripheral nervous system development, suggesting that the Jkappa-RBP gene is also involved in mouse neural development.
To elucidate the functions of Jkappa-RBP gene in mice, we have used homologous recombination in embryonic stem (ES) cells to derive null alleles of the gene. Mouse chimeras produced from two ES clones transmitted the mutated allele to their offspring. Mice with one disrupted Jkappa-RBP allele did not show any overt abnormalities. Heterozygotes for the mutation in the Jkappa-RBP gene were intercrossed to assess the effect of the loss of Jkappa-RBP in homozygous offspring. None of the new born and adult mice were homozygotes suggesting homozygous lethality at embryonic stage. Genotype analysis of embryos shows that homozygous embryos die between day 8.5 and 10.5 of gestation. Homozygous embryos of day 9.5 show 1) anterior opening of neural tube, 2) incomplete turning, and 3) microencephaly.
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Research Products
(4 results)
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[Publications] Tashiro,K.,Tada,H.,Heilker,R.,Shirozu,M.,Nakano,T.and Honjo,T.: "Signal sequence trap: a cloning strategy for secreted protein and type I transmembrane proteins" Science. 261. 600-603 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Aoki,T.,Tashiro,K.,Miyatake,S.,Kinashi,T.,Nakano,T.,Oda,Y.,Kikuchi,H.and Honjo,T.: "Expression of murine interleukin 7 in a murine glioma cell line results in reduced tumorigenicity in vivo" Proc.Natl.Acad.Sci.U.S.A.89. 3850-3854 (1992)
Description
「研究成果報告書概要(和文)」より
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[Publications] Tashiro,K., Tada,H., Heilker,R., Shirozu,M., Nakano,T. and Honjo,T.: "Signal sequence trap : a cloning strategy for secreted protein and type I transmembrane proteins" Science. 261. 600-603 (1993)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Aoki,T., Tashiro,K., Miyatake,S., Kinashi,T., Nakano,T., Oda,Y., Kikuchi,H. and Honjo,T.: "Expression of murine interleukin 7 in a murine glioma cell line results in reduced tumorigenicity in vivo." Proc. Natl. Acad. Sci. U.S.A.89. 3850-3854 (1992)
Description
「研究成果報告書概要(欧文)」より
