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1993 Fiscal Year Final Research Report Summary

The role of stress proteins in the protein biosynthesis.

Research Project

Project/Area Number 04044100
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKyoto University

Principal Investigator

NAGATA Kazuhiro  Kyoto University, Chest Disease Research Institute, professor, 胸部疾患研究所, 教授 (50127114)

Co-Investigator(Kenkyū-buntansha) BENSAUDE O.C.  Ecole Normale Superieure, 主任研究員
WELCH W.J.  University of California, San Francisco, サンフランシスコ校, 教授
MORIMOTO R.I.  Northwestern University, 教授
HIRAYOSHI Kazunori  Kyoto University, Chest Disease Research Institute, 胸部疾患研究所, 助手 (80199108)
Project Period (FY) 1992 – 1993
KeywordsStress protein / Heat shock transcription factor / Molecular Chaperone
Research Abstract

1. Nagata and Morimoto collaborated to investigate the regulatory mechanism of the expression of stress proteins through heat shock transcription factor (HSF). Previously, HSF1 and HSF2 were identified and cloned. We have cloned a cDNA encoding a novel heat shock factor, HSF3, from the cDNA library of chick cells in addition to the chick homologues of human HSF1 and HSF2. HSF3 was activated by heat shock in chick erythrocytic cell line, but not in other chick cells. However, it has been revealed that HSF3 can synergistically cooperate with HSF1 in the activation of stress-induced transcription of stress proteins.
2. Nagata and Hirayoshi investigated the interaction of collagen-specific stress protein, HSP47, with newly synthesized procollagens in the cells. The purpose of the study this year was focused to where the procollagen associates with and dissociates from HSP47 during its biosynthesis. It has been revealed that procollagen binds to HSP47 in the endplasmic reticulum (ER) immediately after the procollagen co-translationally enters the ER, and dissociates from HSP47 in the cis-golgi network after the procollagen is transported into the golgi area.
3. Welch, Bensaude and Morimoto investigated the interaction of HSP70 with newly synthesized proteins as well as with HSFs. During the period of this project, it was figured out that HSP70 associates with activated DNA-binding HSF and renders the dissociation of HSF from the DNA resulting in the attenuation of the HSF activation after the completion of the induction of stress proteins including HSP70.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] N.HOSOKAWA,H.TAKECHI,S.YOKOTA,K.HIRAYOSHI & K.NAGATA: "Structure of the gene encoding mouse 47-kDa heat-shock protein(HSP47)." Gene. 126. 187-193 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.SATOH,A.NAKAI,Y.SOKAWA,K.HIRAYOSHI & K.NAGATA: "Modulation of the phosphorylation of glucose-regulated protein,GRP78,by transformation and inhibition of glycosylation." Exptl.Cell Res.205. 76-83 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.NISHI,W.TAKI,Y.UEMURA,T.HIGASHI,H.KIKUCHI,H.KUDOH,M.SATOH,& K.NAGATA: "Ischemic tolerance due to the induction of HSP70 in a rat ischemic recirculation model." Brain Res.615. 281-288 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.YAMAGUCHI,K.HIRAYOSHI,M.OHKUMA & K.NAGATA: "Enhancement of differentiation induction of mouse myelomonocytic leukemic cells by extracellular ATP." J.Cell.Physiol.(In press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.TAKECHI,N.HOSOKAWA,K.HIRAYOSHI & K.NAGATA: "Alternative 5' splice site selection induced by heat shock." Mol.Cell.Biol.14. 567-575 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.KUDO,K.HIRAYOSHI,Y.KITAGAWA,S.IMAMURA & K.NAGATA: "Two collagen-binding proteins,osteonectin and HSP47,are coordinately induced in transformed keratinocytes by heat and other stresses." Exptl.Cell Res.(In press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.NAGATA & N.HOSOKAWA: "Flavonoids inhibit the expression of heat shock proteins and the acquisition of thermotolerance:Inhibition of the activation of heat shock factor is a key mechanism.In Hyperthermic Oncology 1992" Taylor and Francis Inc., (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 永田 和宏: "ストレス蛋白質-基礎と臨床" 中外医学社, 262 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.HOSOKAWA: "Structure of the gene encoding mouse 47-KDa heat-shock protein (HSP47)." Gene. 126. 187-193 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.SATOH: "Modulation of the phosphorylation of glucose-regulated protein, GRP78, by transformation and inhibition of glycosylation." Exptl. Cell Res.205. 76-83 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.NISHI: "Ischemic tolerance due to the induction of HSP70 in a rat ischemic recirculation model." Brain Res.615. 281-288 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.YAMAGUCHI: "Enhancement of differentiation induction of mouse myelomonocytic leukemic cells by extracellular ATP." J.Cell.Physiol., in press.(1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.TAKECHI: "Alternative 5' splice site selection induced by heat shock." Mol.Cell.Biol.14. 567-575 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.KUDO: "Two collagen-binding proteins, osteonectin and HSP47, are coordinately induced in transformed keratinocytes by heat and other stresses." Exptl. Cell Res., in press.(1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.NAGATA: Taylor and Francis Inc.Flavonoids inhibit the expression of heat shock proteins and the acquisition of thermotolerance : Inhibition of the activation of heat shock factor is a key mechanism. In Hyperthermic Oncology 1992., 57-63 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-02-07  

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