1992 Fiscal Year Final Research Report Summary
Studies on Biological Information and Control in Japan and China
| Project/Area Number |
04045030
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
SANO Seiyo President Shiga University of Medical Science, 学長 (60025533)
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| Co-Investigator(Kenkyū-buntansha) |
る 國強 中華人民共和国, 吉林医学院, 助教
邵 世和 中華人民共和国, 吉林医学院, 講師
りゅう 玉和 中華人民共和国, 吉林医学院, 講師
HUKUDA Sinsuke Professor Shiga University of Medical Science, 医学部, 教授 (20028559)
KANI Kazutaka Professor Shiga University of Medical Science, 医学部, 教授 (60068476)
HATTORI Takanori Professor Shiga University of Medical Science, 医学部, 教授 (70079721)
SETO Akira Professor Shiga University of Medical Science, 医学部, 教授 (00025636)
LU Guo Qiang Research Associate Jilin Medical College
SHAO Shihe Lecuturer Jilin Medical College
LUI Yuhe Lecuturer Jilin Medical College
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| Project Period (FY) |
1992
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| Keywords | Papillomavirus / Papilloma / Squamous cell carcinoma / Gastric cancer / Esophageal cancer / Oncogene |
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| Research Abstract |
This study was undertaken to define a mechanism of carcinogenesis by a cooperative work with Shiga University of Medical Science(SUMS) and Jilin Medical College. Dr. Ryu and Dr. Syo from Jilin Medical College visited SUMS, and studied gastrointestinal tumor pathology and carcinogenesis by virus at Department of Patho-logy (Director Prof. Hattori) and of Microbiology (Director prof. Seto) of SUMS, respectively, for 2 months.
We analyzed the physical states of Shope papillomavirus(SPV) genome in a Shope carcinoma cell line and its four subline ; the sublines had different morphological properties and varied in their potentials for differentiationand tumorigenicity. Vical genomes existed in both episomal and integrated forms in the parental cell line and in three of the four sublines, as revealed by Southern blot analysis of tow-dimensional agarose gel electrophoresis. In the remaining subline, no episomal form was detected. These results suggested that viral genomes in the episomal form c
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ould be integrated into varied chromosomal sites and/or could be lost during cell proliferation. Such integration and/or loss may result in chromosomal aberrations and alterations of cell characteristics. Comparative studies of cellular gene expression were carried out in four sublines. The E6 and E7 transforming genes of the papillomavirus were expressed in all these cell lines, but the expression was the highest in the most tumorigenic and undifferentiated cell line, where th00150 class I expression was the lowest. This down-regulated expression of the MHC class I gene may allow this cell line to evade the immune surveillance.
Characteristics of gastrointestinal carcinoma in Japanese and Chinase.
(1) Histopathological differences of gastric cancers between Japanese and Chinese. Gastric cancers of Japanese were often associated with atrophic change and intestinal metaplasia of the gastri cmucosa. It was also seen in the Chinese of the north-east China, Jilin district. Frequency of esophagus cancer was very high in this area.
(2) Expression of oncogenes in the gastric cancers : An overexpression of c-myc oncogenes was detected both in differentiated adenocarcinoma and undifferentiated carcinomas of the stomach by immunohistochemistry and in situ hybridization. Abnormal c-k-ras oncogene product caused by point mutation was expressd in 20% of cases of early gastric cancers. C-myc and c-k-ras oncogenes were detected in not only gastric carcinomas but colonic carcinomas. Abnormal expression of c-met or k-sam oncogene appers to be highly specific for gastric carcinomas. deletion of c-met oncogene was seen in differentiated adenocarcinomas. On the other hand, onerxpression of k-sam was detected in both poorly differentiated adenocarcinomas and undifferentiated carcinomas. These oncogenes changes were seen not only in Japanese but also in Chinese Gastriccarcinomas. Less
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