1993 Fiscal Year Annual Research Report

昆虫(センチニクバエ)の生体防御機構に関する研究

Research Project

Project/Area Number	04102006
Research Institution	The University of Tokyo
Principal Investigator	名取俊二東京大学, 薬学部, 教授 (50012662)
Co-Investigator(Kenkyū-buntansha)	倉田祥一朗東京大学, 薬学部, 助手 (90221944) 久保健雄東京大学, 薬学部, 講師 (10201469)
Keywords	センチニクバエ / 生体防御 / 個体発生 / 成虫原基 / プロテア-ゼ / 転写因子 / 抗菌性蛋白 / Sarcophagaレクチン
Research Abstract	この特別推進研究は、研究計画調書に記載した5項目の研究計画があるが、本年度はそのいずれの項目についても見るべき成果が得られた。以下に主要な研究実績を記す。1.センチニクバエの成虫原基の分化に必須なプロテア-ゼの同定。(1)カテプシンLは、本来リンゾ-ム酵素として知られるシスティンプロテア-ゼで異物の消化に関与すると考えられているが、センチニクバエの成虫原基がエクダイソン存在下成虫構造に分化する過程で、カテプシンL前駆体を細胞外に分泌すること、分泌された酵素を抗体で阻害すると成虫原基の分化が停止することが判明した。(2)種々の阻害剤を使った研究から、成虫原基の分化には上記のカテプシンL前駆体以外にもう一つ、細胞内のプロリルエンドペプチダ-ゼが関与していることが明らかになった。これらの成果は、当初提出した『昆虫は一つの蛋白を多目的に使う』という仮説を支持するものである。2.Sarcophagaレクチン遺伝子の転写因子の解析。 (1)Sarcophagaレクチン遺伝子の5'上流に存在するNF-κB結合配列に特異的に結合する分子量59kDa蛋白の精製を行い、その性質を明らかにした。(2)同じ遺伝子の5'上流及びイントロンに存在するAT-ストレッチに特異的に結合する分子量53kDa蛋白の精製を行い、そのcDNAの単離に成功し全一次構造を決定した。これらの蛋白はいずれもこの生体防御蛋白遺伝子の転写因子と考えられるものである。3.抗菌性蛋白ザ-ペシンBのフラグメント化。ザ-ペシンBを四つのペプタイドに分割し、各々のペプタイドの抗菌性を調べたところ、7番から17番までの11個のアミノ酸よりなるペプタイドがもとのザ-ペシンBと同等の活性を持つことが判明した。

Research Products
(16 results)

All Other

All Publications (16 results)

[Publications] Katsushige Yamada: "Purification,sequence and antibacterial activityof two novel sapecin homologues from Sarcophaga embryonic cells:similarity of sapecin B to charybdotoxin." Biochem.J.291. 275-279 (1993)
[Publications] Takeo Kubo: "Transient appearance and localization of a 26-kDa lectin,a novel member of the Periplaneta lectin family,in regenerating cocroach leg." Devel.Biol.156. 381-390 (1993)
[Publications] Shafiqul Haq: "Heat-induced DNA relaxation in vitro by mouse DNA topoisomerase I in the presence of ethidium bromide." J.Biochem.113. 620-624 (1993)
[Publications] Hiroshi Hirai: "Inhibition of SV 40 DNA replication in vitro by chlorpromazine" Biol.Pharm.Bull.16. 565-567 (1993)
[Publications] Yasuo Nagasawa: "Purification and heterogeneous localization of Sarcophaga lectin receptor on the surface of imaginal discs of Sarcophaga peregrina(flesh fly)." Develop.Growth & Differ.35. 331-340 (1993)
[Publications] Rosuke Iijima: "Purification,characterization,and cDNA cloning of an antifungal protein from the hemolymph of Sarcophaga peregrina(flesh fly)larvae." J.Biol.Chem.268. 12055-12061 (1993)
[Publications] Nobuaki Adachi: "Purification and characterization of the arylphorin gene specific binding protein from an embryonic cell line of Sarcophaga peregrina(flesh fly)." J.Biochem.114. 55-60 (1993)
[Publications] Tohru Mizushima: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli." Mol.Gen.Genet.238. 1-5 (1993)
[Publications] Ayako Kobayashi: "Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kB-binding motifs of the defense protein genes of Sarcophaga peregrina(flesh fly)." Mol.Cell.Biol.13. 4049-4056 (1993)
[Publications] Rika Nanbu: "Purification of an AU-rich RNA binding protein from Sarcophaga peregrina(flesh fly)and its identification as a thiolase." J.Biochem.114. 432-437 (1993)
[Publications] Kiyoshi Kawasaki: "A novel role of Periplaneta lectin as an opsonin to recoganize 2-keto-3-deoxy ocutonate residues of bacterial lipopolysaccharides." Comp.Biochem.Physiol.106B. 675-680 (1993)
[Publications] Noboru Takahashi: "Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina(flesh fly)." FEBS Lett.334. 153-157 (1993)
[Publications] Shizu Takeda: "Purification of a GTP-binding protein localized in mitochondria." J.Biochem.684. 684-690 (1993)
[Publications] Hideo Iwai: "Solution conformation of an antibacterial peptide,sarcotoxin IA,as determined by ^1H-NMR." Eur.J.Biochem.217. 639-644 (1993)
[Publications] 中西敏行: "新しい二つのS-IIについて-精巣特異的S-IIと酵母S-II-" 実験医学. 11. 55-60 (1993)
[Publications] 名取俊二: "がんに対する生体防御機構非免疫系抗腫瘍性因子を中心として" 共和書院(今西二郎), 12 (1993)

1993 Fiscal Year Annual Research Report

昆虫(センチニクバエ)の生体防御機構に関する研究

Principal Investigator

名取 俊二 東京大学, 薬学部, 教授 (50012662)

Research Products

[Publications] Katsushige Yamada: "Purification,sequence and antibacterial activityof two novel sapecin homologues from Sarcophaga embryonic cells:similarity of sapecin B to charybdotoxin." Biochem.J.291. 275-279 (1993)

[Publications] Takeo Kubo: "Transient appearance and localization of a 26-kDa lectin,a novel member of the Periplaneta lectin family,in regenerating cocroach leg." Devel.Biol.156. 381-390 (1993)

[Publications] Shafiqul Haq: "Heat-induced DNA relaxation in vitro by mouse DNA topoisomerase I in the presence of ethidium bromide." J.Biochem.113. 620-624 (1993)

[Publications] Hiroshi Hirai: "Inhibition of SV 40 DNA replication in vitro by chlorpromazine" Biol.Pharm.Bull.16. 565-567 (1993)

[Publications] Yasuo Nagasawa: "Purification and heterogeneous localization of Sarcophaga lectin receptor on the surface of imaginal discs of Sarcophaga peregrina(flesh fly)." Develop.Growth & Differ.35. 331-340 (1993)

[Publications] Rosuke Iijima: "Purification,characterization,and cDNA cloning of an antifungal protein from the hemolymph of Sarcophaga peregrina(flesh fly)larvae." J.Biol.Chem.268. 12055-12061 (1993)

[Publications] Nobuaki Adachi: "Purification and characterization of the arylphorin gene specific binding protein from an embryonic cell line of Sarcophaga peregrina(flesh fly)." J.Biochem.114. 55-60 (1993)

[Publications] Tohru Mizushima: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli." Mol.Gen.Genet.238. 1-5 (1993)

[Publications] Ayako Kobayashi: "Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kB-binding motifs of the defense protein genes of Sarcophaga peregrina(flesh fly)." Mol.Cell.Biol.13. 4049-4056 (1993)

[Publications] Rika Nanbu: "Purification of an AU-rich RNA binding protein from Sarcophaga peregrina(flesh fly)and its identification as a thiolase." J.Biochem.114. 432-437 (1993)

[Publications] Kiyoshi Kawasaki: "A novel role of Periplaneta lectin as an opsonin to recoganize 2-keto-3-deoxy ocutonate residues of bacterial lipopolysaccharides." Comp.Biochem.Physiol.106B. 675-680 (1993)

[Publications] Noboru Takahashi: "Molecular cloning of cDNA for the 29 kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina(flesh fly)." FEBS Lett.334. 153-157 (1993)

[Publications] Shizu Takeda: "Purification of a GTP-binding protein localized in mitochondria." J.Biochem.684. 684-690 (1993)

[Publications] Hideo Iwai: "Solution conformation of an antibacterial peptide,sarcotoxin IA,as determined by ^1H-NMR." Eur.J.Biochem.217. 639-644 (1993)

[Publications] 中西敏行: "新しい二つのS-IIについて-精巣特異的S-IIと酵母S-II-" 実験医学. 11. 55-60 (1993)

[Publications] 名取俊二: "がんに対する生体防御機構 非免疫系抗腫瘍性因子を中心として" 共和書院(今西二郎), 12 (1993)

名取俊二東京大学, 薬学部, 教授 (50012662)

[Publications] 名取俊二: "がんに対する生体防御機構非免疫系抗腫瘍性因子を中心として" 共和書院(今西二郎), 12 (1993)