1994 Fiscal Year Annual Research Report

昆虫(センチニクバエ)の生体防御機構に関する研究

Research Project

Project/Area Number	04102006
Research Institution	University of Tokyo
Principal Investigator	名取俊二東京大学, 薬学部, 教授 (50012662)
Co-Investigator(Kenkyū-buntansha)	倉田祥一郎 , 薬学部, 助手 (90221944) 久保健雄 , 薬学部, 講師 (10201469)
Keywords	センチニクバエ / 生体防御 / 個体発生 / 抗菌性蛋白 / ザ-ペシン / 成虫原基 / カリウムチンネル / プロテアーゼ
Research Abstract	本年度は最終年度に当たるが、計画調書に記載した2項目の研究を中心に研究を推進した。以下にその主要な研究実績を記す。1.ザ-ペシンBのcDNAクローニングと発現:(!)ザ-ペシンBは抗菌活性とカリウムチャンネル阻害活性を併せ持つ蛋白で、本研究の中心テーマの一つである「二重磯能性」を持つ生体防御蜜臼である。ザ-ペシンBのcDNAを単離した結果、ザ-ペシンBはシグナル配列、プロセグメントおよびザ-ペシンB三部分からなる前駆体が合成され、翻訳後のプロセッシングによって成熟蛋白を生じることが判明した。(2)ザ-ペシンBはザ-ペシンと異なり殆どが脂肪体で合成されることか分かった。(3)ザ-ペシンBは正常な幼虫の脳に一を定量存在することか判明した。この事実は、ザ-ペシンBかザ-ペシンとは明らかに異なる挙動を示し、脳内で何らかの機能を発現していることを示している。これらの結果から、ザ-ペシンBの二重機能性が鮮明になりつつある。2.ザ-ペシンBのαヘリクッス部分を改変した新しい抗菌ペプチドの合成:この研究は予想以上に進展しており、現在アミノ酸9残基よりなるペプチドを数種類取得している。これらのペプチドのあるものは、活性か非常に強く、真菌や線虫に対しても有効であった。この研究は現在このペプチドの実用化へ向けて検討を開始した段階にある。3.脂肪体基底膜上の200kDa蛋白結合部位の同定:この2O0kDa蚤白は体液細胞の表面にあり、自己と非自己を識別する働きを持つと考えられている。(1)脂肪体の膜画分に200kDa蚤臼と特異的に結合する活性があることを明らかにした。(2)ついで、この活性を但う蚤白の精製を試みた。その結果、分子量220kDaと230kDaの蛋白の二重体構造を持つ蛋白が精製された。この蛋白はおそらく、200kDa蛋白のリセプターと思われる。

Research Products
(16 results)

All Other

All Publications (16 results)

[Publications] So Young Leee: "Purification and molecular of CDNA for an inducible antibacterial protein of larvae of a coleopteran insect,Holotrichia diomphalia" J.Biochem.115. 82-86 (1994)
[Publications] Qhiong Xu: "Cloning sand identification of testis-specific transcription elonqation factor S-II" J.Biol.Chem.269. 3100-3103 (1994)
[Publications] Masafumi Shimoda: "Inhibition of the Ca^<2+>-antivated K^+-channel by sapecin B, an insect antibacterial protein" FEBS Letts.339. 59-62 (1994)
[Publications] Katsushige. Yamada: "Characterization of the antibacterial peptide derived from sapecin B,an antibacterial protein of Sarcophaga pereqrina (flesh fly)" Biochem.J.298. 623-628 (1994)
[Publications] Mayumi Matsui: "Purification and characterization of ATBP, a novel protein that bind to A/T stretches in three seqments of Sarcophaqa Iectin qene" Eur.J.Biochem.115. 449-454 (1994)
[Publications] Sumio Ohtsuki: "Aprolyl endopeptidase of Sarcophaqa pereqrina(flesh fly);Its purification and suqqestion for its participation in the differentiation of the imaginal di" J.Biochem. 115. 449-453 (1994)
[Publications] Chalk R: "Purification of an insect defensin from the mosquito, Ades aeqypti" Insect Biochem. Mol. Biol.24. 403-410 (1994)
[Publications] Mitsuko Masutani: "Cloning and functional expression of (ADP-ribose)polymerase cDNA from Sarcophaga Pereqrina" Eur.J.Biochem.220. 607-614 (1994)
[Publications] Jae I1 Kim: "Synthesis and characterization of sapecin snd sapecin B" FEBS Letts.342. 189-192 (1994)
[Publications] Hajime Okuyama: "Selective cessation of protein synthesis in adipocytes produced by dissociating Sarcophaga larval fat body with chymotrypsin" Insect.Biochem.Mol.Biol.24. 525-530 (1994)
[Publications] KOichi Homma: "Purification,characterization,and cDNA cloning of procathepsin L from the culture medium of NIH-Sape-4,and embryonic cell line of Sarcophaqa pereqrina" J.Biol.Chem.269. 15258-15264 (1994)
[Publications] Hyun Joo Moon: "Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvas of the coleopteran,Tenebrio molitor" J.Biochem.302. 53-58 (1994)
[Publications] Juan Alvarcz-Bravo: "Novel synthetic antimicrobial effective against methicillin-resistant Staphylococcus aureus" Biochem J.302. 535-538 (1994)
[Publications] Hiromi Sugiyama: "Induction of qlutathione in the fat body of Sarcophaqa pereqrina by integumental injury" J.Biochem.116. 1171-1175 (1994)
[Publications] Shunji Natori: "Antimicrobial peptides" John wiley &Sons, 283 (1994)
[Publications] Shunji Natori: "Phylogenetic Perspectives in Immunity:Insect Host Defense" R.G.Landes Company, 197 (1994)

1994 Fiscal Year Annual Research Report

昆虫(センチニクバエ)の生体防御機構に関する研究

Principal Investigator

名取 俊二 東京大学, 薬学部, 教授 (50012662)

Research Products

[Publications] So Young Leee: "Purification and molecular of CDNA for an inducible antibacterial protein of larvae of a coleopteran insect,Holotrichia diomphalia" J.Biochem.115. 82-86 (1994)

[Publications] Qhiong Xu: "Cloning sand identification of testis-specific transcription elonqation factor S-II" J.Biol.Chem.269. 3100-3103 (1994)

[Publications] Masafumi Shimoda: "Inhibition of the Ca^<2+>-antivated K^+-channel by sapecin B, an insect antibacterial protein" FEBS Letts.339. 59-62 (1994)

[Publications] Katsushige. Yamada: "Characterization of the antibacterial peptide derived from sapecin B,an antibacterial protein of Sarcophaga pereqrina (flesh fly)" Biochem.J.298. 623-628 (1994)

[Publications] Mayumi Matsui: "Purification and characterization of ATBP, a novel protein that bind to A/T stretches in three seqments of Sarcophaqa Iectin qene" Eur.J.Biochem.115. 449-454 (1994)

[Publications] Sumio Ohtsuki: "Aprolyl endopeptidase of Sarcophaqa pereqrina(flesh fly);Its purification and suqqestion for its participation in the differentiation of the imaginal di" J.Biochem. 115. 449-453 (1994)

[Publications] Chalk R: "Purification of an insect defensin from the mosquito, Ades aeqypti" Insect Biochem. Mol. Biol.24. 403-410 (1994)

[Publications] Mitsuko Masutani: "Cloning and functional expression of (ADP-ribose)polymerase cDNA from Sarcophaga Pereqrina" Eur.J.Biochem.220. 607-614 (1994)

[Publications] Jae I1 Kim: "Synthesis and characterization of sapecin snd sapecin B" FEBS Letts.342. 189-192 (1994)

[Publications] Hajime Okuyama: "Selective cessation of protein synthesis in adipocytes produced by dissociating Sarcophaga larval fat body with chymotrypsin" Insect.Biochem.Mol.Biol.24. 525-530 (1994)

[Publications] KOichi Homma: "Purification,characterization,and cDNA cloning of procathepsin L from the culture medium of NIH-Sape-4,and embryonic cell line of Sarcophaqa pereqrina" J.Biol.Chem.269. 15258-15264 (1994)

[Publications] Hyun Joo Moon: "Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvas of the coleopteran,Tenebrio molitor" J.Biochem.302. 53-58 (1994)

[Publications] Juan Alvarcz-Bravo: "Novel synthetic antimicrobial effective against methicillin-resistant Staphylococcus aureus" Biochem J.302. 535-538 (1994)

[Publications] Hiromi Sugiyama: "Induction of qlutathione in the fat body of Sarcophaqa pereqrina by integumental injury" J.Biochem.116. 1171-1175 (1994)

[Publications] Shunji Natori: "Antimicrobial peptides" John wiley &Sons, 283 (1994)

[Publications] Shunji Natori: "Phylogenetic Perspectives in Immunity:Insect Host Defense" R.G.Landes Company, 197 (1994)

名取俊二東京大学, 薬学部, 教授 (50012662)