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1994 Fiscal Year Final Research Report Summary

Defense mechanism of insect (Sarcophaga peregrina)

Research Project

Project/Area Number 04102006
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Research InstitutionUniversity of Tokyo

Principal Investigator

NATORI Shunji  Tokyo Univ.Pharmaceutical Sci.Prof., 薬学部, 教授 (50012662)

Co-Investigator(Kenkyū-buntansha) KURATA S  Tokyo Univ.Pharmaceutical Sci.Assistant Prof., 薬学部, 助手 (90221944)
KUBO T  Tokyo Univ.Pharmaceutical Sci.Lecturer, 薬学部, 講師 (10201469)
Project Period (FY) 1992 – 1994
KeywordsSarcophaga / Defense system / Development / Antibacterial protein / Sapecin / Imaginal disc / Ptassium channel / Proteinase
Research Abstract

Insect have developed a unique defense system against various outside stimuli such as microbial infection and integmental injury. We have been studying various defense proteins of Sarcophaga peregrina (the flesh fly). These proteins include a lectin and several antibacterial proteins. Throughout these studies, we came to the conclusion that there are two important novel aspects of these defense proteins. One is that a single defense protein probably plays at least two roles in defense and development. Insects probably have the flexibility to use one defense protein in multiple ways. The other interesting aspect is the phylogenetical significance of the insect defense system. Insect defense system is possibly a prototype of the mammalian defense system. Except these two important aspects, we obtained various important findings on insect immnity. Most of these findings have been published.

  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] So Young Leee: "purification and molecular cloning of CNA an inducible antibacterial protein of leavae of a coleopteran insect、Holotrichia diomphalia" J.Biochem.115. 82-86 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Qhiong Xu: "cloning and identification of testis-specific transcription elongation factor s-II" J.Biol.Chem.269. 3100-3103 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masafumi Shimoda: "Inhibition of the Ca^<2+>-activated K^+-channel by sapecin B、an insect antibacterial protein" FEBS Letts.339. 59-62 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsushige Yamada: "Characterization of the antibacterial peptide derived from sapecin B、anantibacterial protein of Sarcophaga peregrina(flesh fly)" Biochem.J.298. 623-628 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mayumi Matsui: "purification and characterization of ATBP、a novel protein that binds to A/T stretches in three segments of sarcophaga lectin gene" Eur.J.Biochem.219. 449-454 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumio Ohtsuki: "A prolyl endopeptidase of Sarcophaga peregrina(flesh fly):Its purification and suggestion for its participation in the differentiation of the imaginal disc" J.Biochem.115. 449-453 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] Shunji Natori: "Antimicrobial peptides" John Wiley & Sons, 283 (1994)

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      「研究成果報告書概要(和文)」より
  • [Publications] Shunji Natori: "phylogenetic perspectives in Immunity: Insect Host Defense" R.G.Landes Company, 197 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanji, T.Mizushima, S.Natori and K.Sekimizu: "Induction by psychotropic drugs and local anesthetics of DnaK and GroEL proteins in Escherichia coli" Biochim.Biophys.Acta. 1129. 172-176 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Jomori and S.Natori: "Function of the lipopolysaccharide-binding protein of Periplaneta americana as an apsonin" FEBS Letts.296. 283-286 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kurata, H.Saito and S.Natori: "Purification of a 29-kDa hemocyte proteinase of Sarcophaga peregrina." Eur.J.Biochem.204. 911-914 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] H.Komano, T.Kurama, Y.Nagasawa and S.Natori: "Evidence for an increase in positive surface charge and an increase in susceptibility to trypsin of Sarcophaga lectin (from the flesh fly, Sarcophaga peregrina) on its interaction with galactose, a hapten sugar of the lectin." Biochem.J.284. 227-230 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] T.Nakanishi, A.Nakano, K.Nomura, K.Sekimizu and S.Natori: "Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae." J.Biol.Chem.267. 13200-13204 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] T.Mizushima, S.Natori and K.Sekimizu: "Inhibition of Escherichia coli DNA topoisomerase I activity by phospholipids." Biochem.J.285. 503-506 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Kurata, H.Saito and S.Natori: "The 29-kDa hemocyte proteinase dissociates fat body at metamorphosis of Sarcophaga." Develop.Biol.153. 115-121 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ishikawa, T.Kubo and S.Natori: "Purification and characterization of a diptericin homologue from Sarcophaga peregrina (flesh fly)." Biochem.J.287. 573-578 (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] A.Nomura, K.Kawasaki, T.Kubo and S.Natori: "Purification and localization of p10, a novel protein that increases in nymphal regenerating legs of Periplaneta americana (American cockroach)." Int.J.Dev.Biol.36. 391-398 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hirai, S.Natori and K.Sekimizu: "Reversal by phosphatidylglycerol and cardiolpin of inhibition of transcription and replication by histones in vitro." Arch.Biochem.Biophys.298. 458-463 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Homma, K.Matsuyama, H.Komano and S.Natori: "The fate of the prosegment in the acute-phase and programd synthesis of sapecin, and antibacterial peptide of the flesh fly (Sarcophaga peregrina)." Biochem.J.288. 281-284 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Saito, S.Kurata and S.Natori: "Purification and characterization of a hemocyte proteinase of Sarcophaga, possibly participating in elimination of foreign substances." Eur.J.Biochem.209. 939-944 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanji, Y.Ohta, S.Kawato, T.Mizushima, S.Natori and K.Sekimizu: "Decrease by physchotropic drugs and local anaesthetics of membrane fluidity measured by fluorescence anisotropy in Escherichia coli." J.Pharm.Pharmcol.44. 1036-1037 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamada and S.Natori: "Purification, sequence and antibacterial activity of two novel sapecin homologues from Sarcophaga embryonic cells : similarity of sapecin B to charybdotoxin." Biochem.J.291. 275-279 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kubo, K.Kawasaki and S.Natori: "Transient appearance and localizaion of a 26-kDa lectin, a novel member of the Periplaneta lectin family, in regenerating cockroack leg." Devel.Biol.156. 381-390 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Haq, S.Natori and K.Sekimizu: "Heat-induced DNA relaxation in vitro by mouse DNA topoisomerase I in the presence of ethidium bromide." J.Biochem.113. 620-624 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hirai, S.Takeda, S.Natori and K.Sekimizu: "Inhibition of SV40 DNA replication in vitro by chlorpromazine." Biol.Pharm.Bull.16. 565-567 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Nagasawa, S.Kurata, H.Komano and S.Natori: "Purification and heterogeneous localizaion of Sarcophaga lectin receptor on the surface of imaginal discs of Sarcophaga peregrina (flesh fly)." Develop.Growth & Differ.35. 331-340 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Iijima, S.Kurata and S.Natori: "Purification, characterization, and cDNA cloning of an antifungal protein from the hemolymph of Sarcophaga peregrina (flesh fly)." J.Biol.Chem.268. 12055-12061 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] N.Adachi, T.Kubo and S.Natori: "Purification and characterization of the arylphorin gene specific binding protein from an embryonic cell line of Sarcophaga peregrina (flesh fly)." J.Biochem.114. 55-60 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Mizushima, S.Natori and K.Sekimizu: "Relaxation of supercoiled DNA associated with induction of heat shock proteins in Escherichia coli." Mol.Gen.Genet.238. 1-5 (1993)

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      「研究成果報告書概要(欧文)」より
  • [Publications] A.Kobayashi, M.Matsui, T.Kubo and S.Natori: "Purification and characterization of a 59-kilodalton protein that specifically binds to NF-kB-binding motifs of the defense protein genes of Sarcophaga peregrina (the flesh fly)." Mol.Cell.Biol.13. 4049-4056 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Nanbu, T.Kubo, T.Hashimoto and S.Natori: "Purification of an AU-rich RNA binding protein from Sarcophaga peregrina (flesh fly) and its identification as a thiolase." J.Biochem.114. 432-437 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kawasaki, T.Kubo and S.Natori: "A novel role of Periplaneta lectin as an opsonin to recognize 2-keto-3-deoxy ocutonate residues of bacterial lipopolysaccharides." Comp.Biochem.Physiol.106B. 675-680 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Takahashi, S.Kurata and S.Natori: "Molecular cloning of cDNA for the 29kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)." FEBS Letts.334. 153-157 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Takeda, Y.Sagara, K.Kita, S.Natori and K.Sekimizu: "Purification of a GTP-binding protein localized in mitochondria." J.Biochem.114. 684-690 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Iwai, Y.Nakajima, S.Natori, Y.Arata and I.Shimada: "Solution conformation of an antibacterial peptide, sarcotoxin IA,as determined by ^1H-NMR." Eur.J.Biochem.217. 639-644 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Y.Lee, H.J.Moon, S.Kurata, T.Kurama, S.Natori and B.L.Lee: "Purification and molecular cloning of cDNA for an inducible antibacterial protein of larvae of a coleopteran insect" Holotrichia diomphalia.J.Biochem.115. 82-86 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Q.Xu, T.Nakanishi, K.Sekimizu and S.Natori: "Cloning and identification of testis-specific transcription elongation factor S-II." J.Biol.Chem.269. 3100-3103 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Shimoda, H.Takagi, S.Kurata, T.Yoshioka and S.Natori: "Inhibition of the Ca^<2+> -activated K^+ -channel by sapecin B,an insect antibacterial protein." FEBS Letts.339. 59-62 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamada and S.Natori: "Characterization of the antimicrobial peptide derived from sapecin B,an antibacterial protein of Sarcophaga peregrina (flesh fly)." Biochem.J.298. 623-628 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Matsui, A.Kobayashi, T.Kubo and S.Natori: "Purification and characterization of ATBP,a novel protein that binds to A/T stretches in three segments of the Sarcophaga lectin gene." Eur.J.Biochem.219. 449-454 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ohtsuki, K.Homma, S.Kurata, H.Komano and S.Natori: "A prolyl endopeptidase of Sarcophaga peregrina (flesh fly) : Its purification and suggestion for its participation in the differentiation of the imaginal discs." J.Biochem.115. 449-453 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Chalk, H.Townson, S.Natori, H.Desmond and P.Ham: "Purification of an insect defensin from the mosquito, Ades aegypti.Insect Biochem." Molec.Biol.24. 403-410 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Masutani, T.Nozaki, Y.Hitomi, M.Ikejima, K.Nagasaki, A.C.De Prati, S.Kurata, S.Natori, T.Sugimura and H.Esumi: "Cloning and functional expression of poly (ADP-ribose) polymerase cDNA from Sarcophaga peregrina." Eur.J.Biochem.220. 607-614 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.I.Kim, H.Iwai, S.Kurata, M.Takahashi, K.Masuda, I.Shimada, S.Natori, Y.Arata and K.Sato: "Synthesis and characterization of sapecin and sapecin B." FEBS Letts.342. 189-192 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Okuyama, S.Kurata and S.Natori: "Selective cessaion of protein synthesis in adipocytes produced by dissociating Sarcophaga larval fat body with chymotrypsin." Insect Biochem.Molec.Biol.24. 525-530 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Homma, S.Kurata and S.Natori: "Purification, characterization, and cDNA cloning of procathepsin L from the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina (flesh fly), and its involvement in the differentiation of imaginal discs." J.Biol.Chem.269. 15258-15264 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.J.Moon, S.Y.Lee, S.Kurata, S.Natori and B.L.Lee: "Purification and molecular cloning of cDNA for an inducible antibacterial protein from larvae of the coleopteran, Tenebrio molitor." J.Biochem.116. 53-58 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] J.A.-Bravo, S.Kurata and S.Natori: "Novel synthetic antimicrobial peptides effective against methicillin-resistant Staphylococcus aureus." Biochem.J.302. 535-538 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Sugiyama and S.: "Induction of glutathione metabolism in the fat body of Sarcophaga peregrina by integumental injury." J.Biochem.116. 1171-1175 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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