1992 Fiscal Year Annual Research Report

動的天然物化学の総合的研究

Research Project

Project/Area Number	04220102
Research Institution	Tohoku University
Principal Investigator	小倉協三東北大学, 反応化学研究所, 教授 (80006303)
Co-Investigator(Kenkyū-buntansha)	砂本順三京都大学, 工学部, 教授 (80037811) 大橋守電気通信大学, 電気通信学部, 教授 (70015535) 杉浦幸雄京都大学, 化学研究所, 教授 (40025698) 楠本正一大阪大学, 理学部, 教授 (30028253) 市原耿民北海道大学, 農学部, 教授 (20000820)
Keywords	動的天然物化学 / 精密構造認識 / 生体低分子 / 糖質 / 核酸 / 酵素 / 励起分子 / 細胞表層
Research Abstract	最終年度に際し、一属成果を上げるため、班員相互の討議と情報交換を活発に行ないながら研究を推進した。その間、新らたに班員間での共同研究は14件に増加し、優れた成果を収めた。本年度は、大阪において第5回総括班会議、班会議、全体会議および第4回公開シンポジウムを6月15〜16日を開催した。また、各班員に重点領域研究の意義と推進方法を徹底させるとともに、各班の研究計画の説明を行なった。さらに、8月2日〜5日の4日間に亘り、宮城県蔵王において合同班会議を開いた。各自研究の進捗状況とその最終年度の研究計画を詳細に説明し、活発な討論を行ない情報交換の実を充分に挙げることができた。また、8月4日には第6回総括班会議を行ない、最終年に向けての領域全体の活動方針並びに成果とりまとめのやり方等について議論した。第5回公開シンポジウムは東京において12月1〜2日に行ない、これにより全班員のシンポジウム講演を終了した。さらにこの期間中に第7回総括班会議、班会議を開催し、成果とりまとめと出版について方針を決定した。協同利用設備として、名古屋大学農学部に設置されている分子構造解析用コンピューターが随時班員の利用にあてられて、協同利用の実を挙げた。これによれば複雑な構造の化合物の安定な形や、分子間相互の存在状態の計算、さらに核磁気共鳴装置を用いた2次元NOE測定値からの距離情報と連動させることにより計算値を実験値と対比させることができるので、極めて有効に活用された。また、1次元の分解能向上のためにデーター変換端末システムおよびソフトウエアーを追加設置することにより、分子計算とNMR解析が同時に可能となり一層能率化した。

Research Products
(23 results)

All Other

All Publications (23 results)

[Publications] H.Sagami: "Studies on Geranylgeranyl Diphosphate Synthase from Rat Liver" Arch.Biochem.Biophys.297. 314-320 (1992)
[Publications] T.Gotoh: "Farnesyl Diphosphate Synthase and Solanesyl Diphosphate Synthase Reactions of Diphosphhate-Modified Allylic Analogs" J.Biochem.112. 20-27 (1992)
[Publications] H.Sagami: "Separation of Dolichol from Dehydrodolichol by a Simple Two-Plate Thein-Layer Chromatography" J.Lipid.Res.33. 1857-1862 (1992)
[Publications] S.Ohnuma: "Chain Length Distribution of the Products Formed in Solanesyl Diphosphate Synthase Reaction" J.Biochem.112. 743-749 (1992)
[Publications] T.Koyama: "Thermostable Farnesyl Diphosphate Synthase of Bacillus Stearothermophilus:Molecular Cloning,Sequence Determination,Overproduction,and Purification" J.Biochem.113. 355-363 (1993)
[Publications] H.Sagami: "Formation of Dolichol from Dehydrodolichol is Catalyzed by NADPH-Dependent Reductase Localized in Microsomes of Rat Liver" J.Biol.Chem.
[Publications] H.Oikawa: "Biosynthetic Study of Chaetoglobosin A:Origins of the Oxygen and Hydrogen Atoms,and Indirect Evidence for Biological Diels-Alder Reaction" J.Chem.Soc.,Perkin Trans.I. 2955-2959 (1992)
[Publications] 市原耿民: "先導的バイオサイエンスのための有機合成化学的展開ー生体内Diels-Alder反応の可能性ー" 有機合成化学協会誌. 50. 96-111 (1992)
[Publications] H.-M.Kuhn: "Characterization of Epitope Specificity of Murine Monoclonal Antibodies Directed against Lipid A" Infect.Immun.60. 2201-2210 (1992)
[Publications] K.Fukase: "Synthetic Study on Lipoteichoic Acid of Gram Negative Bacteria.1.Synthesis of Proposed Fundamental Structure of Streptococcus pyogenes Lipoteichoic Acid" Bull.Chem.Soc.Jpn.65. 2643-2654 (1992)
[Publications] A.Ichikawa: "Activation of DNA Cleavage by Dynemicin A in a B-Z Conformational Junction" Biochemistry. 31. 6784-6787 (1992)
[Publications] T.Shiraki: "C-1'Hydrogen Abstraction of Deoxyribose in DNA Strand Scission by Dynemicin A" Biochem.Biophys.Res.Commun.188. 584-589 (1992)
[Publications] M.Uesugi: "DNA Cleaving Modes in Minor Groove of DNA Helix by Esperamicin and Calicheamicin Antitumor Antibiotics" Nucleic Acids Res.(Sym.Ser.). 27. 1-2 (1992)
[Publications] M.Tokuda: "Synthesis of a Hybrid Molecule Containing Neocarzino-statin Chromophore Analogue and Minor Groove Binder" Tetrachedron Lett.34. 669-672 (1993)
[Publications] S.Kurono: "Detection of Molecular Ions of Cyclodextrin Inclusion Complexes" Org Mass Spectrom.27. 1157-1160 (1992)
[Publications] K.Tsujimoto: "Stereoselective Photoisomerization of 4-Retinal" J.Photochem.Phtobiol.A:Chem.65. 73-78 (1992)
[Publications] T.Hirano: "Chemiluminescence of Coelenterazine Analogues-Struc-tures of Emitting Species" Tetrahedron Letters. 33. 5771-5774 (1992)
[Publications] Y.Ohmiya: "Two Excited States in Acquorin Bioluminescence Induced by Tryptophan Modification" FEBS Lett.301. 197-201 (1992)
[Publications] C.F.Qi: "Chemi-and Bioluminescence of Coelenterazine Analogues with Phenyl Homologues an the C-2 Position" J.Chem.Soc.Perkin Trans I. 1607-1611 (1992)
[Publications] F.Q.Chen: "3-Benzoyl-5-(4-hydroxyphenyl)-2-[4-(1-azi-2,2,2-trifluoroethyl)phenylacetamido]phrazine.A Photoreactive Analogue of Coelenteramide.Synthesis and Photolysis" Chemistry Lett.287-290 (1993)
[Publications] M.Goto: "Effect of Artificial Boundary Lipid on the Membrane Dynamics of Human Glycophorin-Containing Liposome" Bull.Chem.Soc.Jpn.65. 3331-3334 (1992)
[Publications] J.Sunamoto: "Direct Transfer of Membrane Proteins from B16 Melanoma Cell to Artificial Cell Liposome" Proc.Japan Acad.68. 69-74 (1992)
[Publications] J.Sunamoto: "Effective Reconstitution of Cell Membrane Proteins into Artificial Cell Liposomes" Progress in Pacific Polymer Sciences. 2. 223-233 (1992)

1992 Fiscal Year Annual Research Report

動的天然物化学の総合的研究

Principal Investigator

小倉 協三 東北大学, 反応化学研究所, 教授 (80006303)

Research Products

[Publications] H.Sagami: "Studies on Geranylgeranyl Diphosphate Synthase from Rat Liver" Arch.Biochem.Biophys.297. 314-320 (1992)

[Publications] T.Gotoh: "Farnesyl Diphosphate Synthase and Solanesyl Diphosphate Synthase Reactions of Diphosphhate-Modified Allylic Analogs" J.Biochem.112. 20-27 (1992)

[Publications] H.Sagami: "Separation of Dolichol from Dehydrodolichol by a Simple Two-Plate Thein-Layer Chromatography" J.Lipid.Res.33. 1857-1862 (1992)

[Publications] S.Ohnuma: "Chain Length Distribution of the Products Formed in Solanesyl Diphosphate Synthase Reaction" J.Biochem.112. 743-749 (1992)

[Publications] T.Koyama: "Thermostable Farnesyl Diphosphate Synthase of Bacillus Stearothermophilus:Molecular Cloning,Sequence Determination,Overproduction,and Purification" J.Biochem.113. 355-363 (1993)

[Publications] H.Sagami: "Formation of Dolichol from Dehydrodolichol is Catalyzed by NADPH-Dependent Reductase Localized in Microsomes of Rat Liver" J.Biol.Chem.

[Publications] H.Oikawa: "Biosynthetic Study of Chaetoglobosin A:Origins of the Oxygen and Hydrogen Atoms,and Indirect Evidence for Biological Diels-Alder Reaction" J.Chem.Soc.,Perkin Trans.I. 2955-2959 (1992)

[Publications] 市原 耿民: "先導的バイオサイエンスのための有機合成化学的展開ー生体内Diels-Alder反応の可能性ー" 有機合成化学協会誌. 50. 96-111 (1992)

[Publications] H.-M.Kuhn: "Characterization of Epitope Specificity of Murine Monoclonal Antibodies Directed against Lipid A" Infect.Immun.60. 2201-2210 (1992)

[Publications] K.Fukase: "Synthetic Study on Lipoteichoic Acid of Gram Negative Bacteria.1.Synthesis of Proposed Fundamental Structure of Streptococcus pyogenes Lipoteichoic Acid" Bull.Chem.Soc.Jpn.65. 2643-2654 (1992)

[Publications] A.Ichikawa: "Activation of DNA Cleavage by Dynemicin A in a B-Z Conformational Junction" Biochemistry. 31. 6784-6787 (1992)

[Publications] T.Shiraki: "C-1'Hydrogen Abstraction of Deoxyribose in DNA Strand Scission by Dynemicin A" Biochem.Biophys.Res.Commun.188. 584-589 (1992)

[Publications] M.Uesugi: "DNA Cleaving Modes in Minor Groove of DNA Helix by Esperamicin and Calicheamicin Antitumor Antibiotics" Nucleic Acids Res.(Sym.Ser.). 27. 1-2 (1992)

[Publications] M.Tokuda: "Synthesis of a Hybrid Molecule Containing Neocarzino-statin Chromophore Analogue and Minor Groove Binder" Tetrachedron Lett.34. 669-672 (1993)

[Publications] S.Kurono: "Detection of Molecular Ions of Cyclodextrin Inclusion Complexes" Org Mass Spectrom.27. 1157-1160 (1992)

[Publications] K.Tsujimoto: "Stereoselective Photoisomerization of 4-Retinal" J.Photochem.Phtobiol.A:Chem.65. 73-78 (1992)

[Publications] T.Hirano: "Chemiluminescence of Coelenterazine Analogues-Struc-tures of Emitting Species" Tetrahedron Letters. 33. 5771-5774 (1992)

[Publications] Y.Ohmiya: "Two Excited States in Acquorin Bioluminescence Induced by Tryptophan Modification" FEBS Lett.301. 197-201 (1992)

[Publications] C.F.Qi: "Chemi-and Bioluminescence of Coelenterazine Analogues with Phenyl Homologues an the C-2 Position" J.Chem.Soc.Perkin Trans I. 1607-1611 (1992)

[Publications] F.Q.Chen: "3-Benzoyl-5-(4-hydroxyphenyl)-2-[4-(1-azi-2,2,2-trifluoroethyl)phenylacetamido]phrazine.A Photoreactive Analogue of Coelenteramide.Synthesis and Photolysis" Chemistry Lett.287-290 (1993)

[Publications] M.Goto: "Effect of Artificial Boundary Lipid on the Membrane Dynamics of Human Glycophorin-Containing Liposome" Bull.Chem.Soc.Jpn.65. 3331-3334 (1992)

[Publications] J.Sunamoto: "Direct Transfer of Membrane Proteins from B16 Melanoma Cell to Artificial Cell Liposome" Proc.Japan Acad.68. 69-74 (1992)

[Publications] J.Sunamoto: "Effective Reconstitution of Cell Membrane Proteins into Artificial Cell Liposomes" Progress in Pacific Polymer Sciences. 2. 223-233 (1992)

小倉協三東北大学, 反応化学研究所, 教授 (80006303)

[Publications] 市原耿民: "先導的バイオサイエンスのための有機合成化学的展開ー生体内Diels-Alder反応の可能性ー" 有機合成化学協会誌. 50. 96-111 (1992)