1993 Fiscal Year Annual Research Report

生体関連金属錯体における多重相互作用と機能発現の研究

Research Project

Project/Area Number	04225102
Research Institution	Nagoya University
Principal Investigator	山内脩名古屋大学, 理学部, 教授 (70029643)
Co-Investigator(Kenkyū-buntansha)	松本和子早稲田大学, 理工学部, 教授 (60111457) 宗像惠近畿大学, 理工学部, 教授 (80090942) 鈴木晋一郎大阪大学, 教養部, 教授 (70116052) 杉浦幸雄京都大学, 化学研究所, 教授 (40025698)
Keywords	多重相互作用 / 分子間相互作用 / 電子移動反応 / キサンチン酸化酵素 / プテリン補酵素 / 転写因子Sp1 / アルツハイマー病 / 神経成長因子
Research Abstract	本研究の最終年度であるため、生体系および生体関連金属錯体における多重相互作用による機能発現を中心に、金属イオン配位圏での分子間相互作用、金属タンパク質の構造、情報伝達の諸点から研究を行った。 1.多重相互作用の生体系での意義生体系金属錯体について、アミノ酸金属錯体をモデルとする生体ポリアミン関与の分子間水素結合様式、ヒト転写因子亜鉛フィンガーの特異構造とDNA認識、低分子金属錯体によるDNA結合、芳香族アミノ酸の示す分子間相互作用をそれぞれ明らかにした。また、ターゲットとする白金ブルー錯体の示す反応様式を明らかにした。一方、植物ホルモンであるエチレンの受容体を、銀イオンをマーカーとして検索する方法を開拓した。 2.酵素モデル反応系の神経成長因子(NGF)生成促進作用銅含有フェニルアラニンヒドロキシラーゼモデル系でのプテリン補酵素との酸化還元反応過程の詳細を調べ、酸素の活性化メカニズムの情報を得た。また、この系によるフェニルアラニンの水酸化反応生成物をマウス神経細胞に作用させ、著しいNGF生成促進作用を見出した。この作用へのアルミニウム(III)の抑制効果から、アルツハイマー病の原因としてのアルミニウムの可能性を推定した。 3.金属錯体とDNAとの相互作用銅含有の亜硝酸還元酵素の反応メカニズムを追究し、硝酸イオン還元の電子移動過程を推定した。鉄クラスター系を用いた研究から、電子移動に伴う構造変化を明らかにした。一方、モリブデン含有のキサンチン酸化酵素を結晶として単離し、分子内電子伝達経路を明らかにすると共に、X線回折像をはじめて得た。

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] H.Masuda: "Structures and Stabilities of Ternary Copper(II) Complexes Containing an Acidic and a Basic Amino Acid.Evidence for Arginine Side Chain Involvement in Intermolecular Interactions and Its Biological Implication" Inorg.Chem.32. 1111-1118 (1993)
[Publications] Osamu Yamauchi: "Ternary Metal(II) Complexes with Tyrosine-Containing Dipeptides.Structures of Copper(II) and Palladium(II) Complexes Involving L-Tyrosylglycine and Stabilization of Copper(II) Complexes Due to Intramolecular Aromatic Ring Stacking." Inorg.Chem.32. 4951-4959 (1993)
[Publications] Akira Odani: "Structures and Properties of Tenary Copper(II) Complexes Involving a Halogen Substituted Phenylalanine and 2,2'-Bipyridine.Intermolecular Interactions between Halo and Organic Groups." Bull.Chem.Soc.Jpn.67. 131-137 (1994)
[Publications] Akira Odani: "Spectroscopic Evidence for the Redox Reaction between Copper(II) and a Pterin Cofactor Model,6,7-Dimethyl-5,6,7,8-tetrahydropterin." Chem.Lett.385-388 (1994)
[Publications] L.Lomozik: "Spectroscopic studies on complex formation and noncovalent interactions in ternary palladium(II) systems involving spermidine and 2,3-diaminopropionate of 2,4-diaminobutyrate." Inorg.Chim.Acta. 219. 107-114 (1994)
[Publications] T.Inoue: "Crystallization and Preliminary X-ray Studies on Pseudoazurin from Achromobacter cycloclastes IAM 1013." J.Biochem.114. 761-762 (1993)
[Publications] N.Nakamura: "Electrochemical Properties of Coenzyme Pyrroloquinolinequinone Using a Di-(4-pyridyl) Dislfide Modified Gold Electrode under Acidic Conditions." Bull.Chem.Soc.Jpn.66. 1289-1291 (1993)
[Publications] T.Kohzuma: "Direct Electrochemistry of Copper-Containing Nitrite Reductase from Achromobacter xylosoxidans NCIB 11015." Chem.Lett.12. 2029-2032 (1993)
[Publications] S.Itoh: "Pysicochemical Properties of 2-Methylthio-4-methylphenol,a Model Compound of the Novel Cofactor of Galactose Oxidase." Chem.Lett.12. 2099-2102 (1993)
[Publications] N.Nakamura: "Structure of a Tripotassium Pyrroloquinoline Quinone Salt." Acta Cryst.2093-2095 (1993)
[Publications] Y.Nakao: "Preparation and Properties of the Dinuclear Copper(II) Complexes Bridged by an Alkoxo and an Exogenous Bridging Ligand." Bull.Chem.Soc.Jpn.67. 260-262 (1994)
[Publications] T.Kohzuma: "Direct Electrochemistry of Nitrite Reductase from Achromobacter cycloclastes IAM 1013." Bull.Chem.Soc.Jpn.138-143 (1994)
[Publications] K.D.Karlin: "Bioinorganic Chemistry of Copper" Copper(II)-Pteridine Chemistry.Structures,Properties,and Phenylalanine Hydroxylase Models., (1993)

1993 Fiscal Year Annual Research Report

生体関連金属錯体における多重相互作用と機能発現の研究

Principal Investigator

山内 脩 名古屋大学, 理学部, 教授 (70029643)

Research Products

[Publications] H.Masuda: "Structures and Stabilities of Ternary Copper(II) Complexes Containing an Acidic and a Basic Amino Acid.Evidence for Arginine Side Chain Involvement in Intermolecular Interactions and Its Biological Implication" Inorg.Chem.32. 1111-1118 (1993)

[Publications] Osamu Yamauchi: "Ternary Metal(II) Complexes with Tyrosine-Containing Dipeptides.Structures of Copper(II) and Palladium(II) Complexes Involving L-Tyrosylglycine and Stabilization of Copper(II) Complexes Due to Intramolecular Aromatic Ring Stacking." Inorg.Chem.32. 4951-4959 (1993)

[Publications] Akira Odani: "Structures and Properties of Tenary Copper(II) Complexes Involving a Halogen Substituted Phenylalanine and 2,2'-Bipyridine.Intermolecular Interactions between Halo and Organic Groups." Bull.Chem.Soc.Jpn.67. 131-137 (1994)

[Publications] Akira Odani: "Spectroscopic Evidence for the Redox Reaction between Copper(II) and a Pterin Cofactor Model,6,7-Dimethyl-5,6,7,8-tetrahydropterin." Chem.Lett.385-388 (1994)

[Publications] L.Lomozik: "Spectroscopic studies on complex formation and noncovalent interactions in ternary palladium(II) systems involving spermidine and 2,3-diaminopropionate of 2,4-diaminobutyrate." Inorg.Chim.Acta. 219. 107-114 (1994)

[Publications] T.Inoue: "Crystallization and Preliminary X-ray Studies on Pseudoazurin from Achromobacter cycloclastes IAM 1013." J.Biochem.114. 761-762 (1993)

[Publications] N.Nakamura: "Electrochemical Properties of Coenzyme Pyrroloquinolinequinone Using a Di-(4-pyridyl) Dislfide Modified Gold Electrode under Acidic Conditions." Bull.Chem.Soc.Jpn.66. 1289-1291 (1993)

[Publications] T.Kohzuma: "Direct Electrochemistry of Copper-Containing Nitrite Reductase from Achromobacter xylosoxidans NCIB 11015." Chem.Lett.12. 2029-2032 (1993)

[Publications] S.Itoh: "Pysicochemical Properties of 2-Methylthio-4-methylphenol,a Model Compound of the Novel Cofactor of Galactose Oxidase." Chem.Lett.12. 2099-2102 (1993)

[Publications] N.Nakamura: "Structure of a Tripotassium Pyrroloquinoline Quinone Salt." Acta Cryst.2093-2095 (1993)

[Publications] Y.Nakao: "Preparation and Properties of the Dinuclear Copper(II) Complexes Bridged by an Alkoxo and an Exogenous Bridging Ligand." Bull.Chem.Soc.Jpn.67. 260-262 (1994)

[Publications] T.Kohzuma: "Direct Electrochemistry of Nitrite Reductase from Achromobacter cycloclastes IAM 1013." Bull.Chem.Soc.Jpn.138-143 (1994)

[Publications] K.D.Karlin: "Bioinorganic Chemistry of Copper" Copper(II)-Pteridine Chemistry.Structures,Properties,and Phenylalanine Hydroxylase Models., (1993)

山内脩名古屋大学, 理学部, 教授 (70029643)