• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

Molecular Diagnosis of Human Leukemias

Research Project

Project/Area Number 04253208
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionUniversity of Tokyo

Principal Investigator

HIRAI Hisamaru  University of Tokyo, 3rd Dept.of Internal Med., Lecturer, 医学部(病), 講師 (90181130)

Co-Investigator(Kenkyū-buntansha) OGAWA Seishi  University of Tokyo, 3rd Dept.of Internal Med., Medical Fellow, 医学部(病), 医員
MITANI Kinuko  University of Tokyo, 3rd Dept.of Internal Med., Assistant Pro., 医学部(病), 助手 (50251244)
Project Period (FY) 1992 – 1993
Keywordst(3 ; 21) translocation / chronic myelocytic leukemia / blastic crisis / AML1 / EVI-1 chimeric gene / AML1 gene / EVI-1 gene / transcription factor
Research Abstract

The t(3 ; 21)(q26 ; q22) translocation is thought to trigger blastic crisis in chronic myelocytic leukemia (CML). This reciprocal translocation generates an AML1/EVI-1 chimeric gene which is transcribed as fusion mRNAs. The 180 kD AML1/EVI-1 fusion protein contains an amino-terminal half of AML1 including a runt homology domain which is fused to the entire of zinc finger EVI-1 protein. The AML1/EVI-1 fusion transcript is consistent among all three cases of the t(3 ; 21)-carrying leukemia examined. Synthetic antisense oligonucleotides complementary to the sequences including the transcriptional initiation sequences of authentic AML1 and EVI-1 specifically suppress growth of leukemia cells with the t(3 ; 21) translocation. These findings strongly suggest that the t(3 ; 21) translocation results in the formation of a new class of a chimeric transcription factor which could contribute to leukemic progression through interference with cell growth and differentiation.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Toyoshima H: "Differently spliced cDNAs of human ltk receptor tyrosine kinase predeict receptor proteins with and without tyrosine kinase domain and a soluble receptor protein." Proc.Natl.Acad.Sci.USA. 90. 5404-5408 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugimoto K: "Mutations of the p53 gene in myelodysplastic syndromes and MDS-derived leukemia." Blood. 81. 3022-3026 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanazono Y: "c-fps/fes protein-tyrosine kinase is implicated in a signaling pathway triggered by granulocyte-macrophage colony-stimulating factor and interleukin-3." EMBO J.12. 1641-1646 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogawa S: "Detection of the PML/RAR α fusion gene in acute promyelocytic leukemia with a complex translocation involving chromosome 15,17,and 18." Cancer Genet.Cytogenet.69. 113-117 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Amano M: "Rearrangement of retinoic acid receptor α and PML in promyelocytic blast crisis of Ph1 chromosome positive chronic myelocytie leukemia with normal copies of chromosome 15." Blood. 81. 2469-2470 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mitani K: "Generation of the AML1/Evi-1 fusion gene in the t(3;21)(q26;q22) causes blastic crisis in chronic myelocytic leukemia."

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoshima H,Kozutsumi H,Maru Y,Hagiwara K,Furuya A,Mioh H,Hanai N,Takaku F,Yazaki Y,Hirai H: "Differently spliced cDNAs of human 1tk receptor tyrosine kinase predict receptor proteins with and without tyrosine kinase domain and a soluble receptor protein" Proc.Natl.Acad.Sci.USA. 90. 5404-5408 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugimoto K,Hirano N,Toyoshima H,Chiba S,Mano H,Takaku F,Yazaki Y,Hirai H: "Mutations of the p53 gene in myelodysplastic syndromes and MDS-derived leukemia" Blood. 81. 3022-3026 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanazono Y,Chiba S,Sasaki K,Mano H,Miyajima A,Arai K,Yazaki Y,Hirai H: "c-fps/fes protein-tyrosine kinase is implicated in a signaling pathway triggered by granulocyte-macrophage colony-stimulating factor and interleukin-3" EMBO J.12. 1641-1646 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanazono Y,Chiba S,Sasaki K,Mano H,Yazaki Y,Hirai H: "Erythropoietin induces tyrosine phosphorylation and kinase activity of the c-fps/fes proto-oncogene product in human erythropoietin-responsive cells" Blood. 81. 3193-3196 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sasaki K,Chiba S,Hanazono Y,Mano H,Yazaki Y,Hirai H: "Coordinate expression of the alpha and beta chains of human granolocyte-macrophage colony-stimulating factor receptor confers ligand-induced morphological transformation in mouse fibroblasts" J.Biol.Chem.268. 13697-13702 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogawa S,Mitani K,Sato Y,Sugimoto K,Toyoshima H,Mano H,Takaku F,Yazaki Y,Hirai H: "Detection of the PML/RARalpha fusion gene in acute promyelocytic leukemia with a complex translocation involving chromosome 15,17, and 18" Cancer Genet.Cytogenet.69. 113-117 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Amano M,Togawa A,Tsurugano S,Soda Y,Yuo A,Takaku F,Ogawa S,Hirai H,Yamada K: "Rearrangement of retinoic acid receptor alpha and PML in promyelocytic blast crisis of Phl chromosome positive chronic myelocytic leukemia with normal copies of chromosome 15" Blood. 81. 2469-2470 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mitani K,Ogawa S,Miyoshi H,Kurokawa M,Mano H,Yazaki Y,Ohki M,Hirai H: "Generation of the AML1/Evi-1 fusion gene in the t(3 ; 21)(q26 ; q22) causes blastic crisis in chronic myelocytic leukemia" EMBO. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi