1992 Fiscal Year Annual Research Report

細胞複製制御の分子生物学的研究

Research Project

Project/Area Number	04256101
Research Institution	Nagoya University
Principal Investigator	岡崎恒子名古屋大学, 理学部, 教授 (10022584)
Co-Investigator(Kenkyū-buntansha)	山本正幸東京大学, 理学部, 教授 (40114706) 花岡文雄理化学研究所, 細胞生理, 主任研究員 (50012670) 平賀壮太熊本大学, 医学部, 教授 (40027321) 吉川寛大阪大学, 医学部, 教授 (70019876)
Keywords	ゲノム複製の開始 / ゲノム複製の終結 / ゲノムの分配 / 細胞周期 / 増殖制御とがん化 / 増殖制御と分化 / 増殖制御の情報伝達 / 複製酵素
Research Abstract	本重点領域研究は、原核生物並びに真核生物細胞複製における制御の仕組みを分子レベルで総合的に理解することを研究目的としている。そのため「ゲノム複製開始の制御」、「ゲノム複製の終結と分配の制御」、「細胞複製制御の遺伝子発現」の3課題について班員33名からなる計画研究班を組織し、公募研究24課題と共に領域研究の重点的推進をはかった。平成4年度は班運営のため幹事会を5回開催しきめ細かい総括班の活動につとめた。8月に連続班会議を開催し班員の研究の進展を知り相互理解と連携をはかった。又この折に総括班会議を開催し本年度の活動方針を決定した。11月には真核細胞染色体の複製分野において世界をリ-ドするJ.A.Huberman博士(米)を招聘し、最新情報と研究動向について本重点領域班員との間で討議を行い、又今後の研究に対する助言も得た。名古屋大学で公開シンポジウム「真核細胞ゲノムの複製と分配のしくみ」を開催(11月)、班員8名の講演とHuberman博士(米国)の特別講演によりこの研究分野の一般へのアピ-ルを行った。領域研究の推進、人材発掘、若手研究者の育成などのために、「原核生物における細胞複製制御」(8月)、「DNA複製ワ-クショップ」(11月)(Huberman博士も参加)、「細胞制御ワ-クショップ」(1月)を開催した。又班員間の連携と情報交流のために7月、10月、3月の3回サ-キュラ-を発行した。8月には各班員の研究の進展状況をまとめた報告集を作り、全班員に配布し、研究の相互理解を深め、かつ来年以降の総括班の運営資料とした。12月に第2回総括班会議を開催し、本年度の活動状況と来年度の計画の検討を行った。研究拠点となる研究室には細胞成分の分離精製解析のための多機能超遠心機、DNA配列決定と解析のためのDNAシ-クエンサ-、DNAシンセサイザ-、PCR等を設置し、研究の推進に大いに役立った。

Research Products

(26 results)

All Other

All Publications (26 results)

[Publications] Y.Muro: "Centromere protein B assembles human centromeric α-satellite DNA at the 17-bp sequence,CENP-B box." J.Cell Biol.116. 585-596 (1992)
[Publications] K.Yoda: "A human centromere protein,CENP-B,has a DNA binding domain containing four potential α helices at the NH_2 terminus,which is separable from dimerizing activity." J.Cell Biol.119. 1413-1427 (1992)
[Publications] K.Shirahige: "Location and characterization of autonomously replicating sequences (ARSs) from chromosome VI of Saccharomyces cerevisiae." Mol.Cell.Biol.
[Publications] K.J.Begg: "Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutants." J.Bacteriol.174. 2416-2417 (1992)
[Publications] S.Hiraga: "Chromosome and plasmid partition in Escherichia coli." Ann.Rev.Biochem.61. 283-306 (1992)
[Publications] R.Imamura: "Characterization of high molecular weights of complexes and polymers of cytoplasmid proteins in Escherichia coli." Res.Microbiol.143. 743-753 (1992)
[Publications] K.Yamanaka: "Identification and characterization of the smbA gene,a suppressor of the muB null mutant of Escherichia coli." J.Bacteriol.174. 7517-7526 (1992)
[Publications] H.Niki: "E.coli MukB protein involved in chromosome partition forms a homodimer with a rod-and-hinge structure having DNA binding and ATP/GTP binding activities." EMBO J.11. 5101-5109 (1992)
[Publications] Y.Ishimi: "Topoisomerase II plays an essential role as a sivelase in the late stage of SV40 chromosome replication in vitro." J.Biol.Chem.267. 462-466 (1992)
[Publications] K.Sugasawa: "Non-conservative segregation of parental nucleosomes during simian virus 40 chromosome replication in vitro." Proc.Natl.Acad.Sci.USA. 89. 1055-1059 (1992)
[Publications] D.Ayusawa: "Complementation by a cloned human ubiquitin-activating enzyme El of the S-phase-arrested mouse FM3A cell mutant with thermolabile El." Cell Struct.Funct.17. 113-122 (1992)
[Publications] K.Ozawa: "Mapping of the human GSPTl gene,a human homolog of the yeast GST1 gene,to chromosomal band 16p13.1." Somat.cell Mol.Genet.18. 189-194 (1992)
[Publications] F.Yatagai: "Resolution and characterization of polymorphic DNA by SSCP and chemical cleavage methodologies." J.Rad.Res.33. 95-108 (1992)
[Publications] T.H.Norwood: "A Somatic genetic approach to the analysis of senescence in human diploid fibroblast in vitro:from heterokaryons to molecules." Exp.Gerontol.27. 391-395 (1992)
[Publications] H.Kamiya: "An abasic site analogue activates a c-Ha-ras gene by a point mutation at modified and adjacent positions." Nucleic Acids Res.20. 4409-4415 (1992)
[Publications] T.Sudo: "The c-myb proto-oncogene product binds to bu does not activate the promoter of the DNA polymerase α gene." Oncogene. 7. 1999-2006 (1992)
[Publications] N.Iwai: "Cloning and sequence of a functionally active cDNA for mouse ubiquitin-activating enzyme E." Gene. 118. 279-282 (1992)
[Publications] K.Segawa: "Abrogation of p53-mediated transactivation by SV40 large T antigen." Oncogene.
[Publications] H.Miyazawa: "Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase α-primase complex and their gene expression during cell proliferation and the cell cycle." J.Biol.Chem.268. (1993)
[Publications] K.Sugasawa: "Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts.I.Develpment of a cellfree system detecting excision repair of UV-irradiated SV40 chromosomes." J.Biol.Chem.268. (1993)
[Publications] C.Matsutani: "Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts.II.Defective DNA repair synthesis by xeroderma pigmentosum cell extracts." J.Biol.Chem.268. (1993)
[Publications] K.Matsumoto: "TAF-I,a novel host factor required to stimulate the adenovirus core DNA replication." J.Biol.Chem.268. (1993)
[Publications] Y.Imai: "S.pombe sxa1^+ and sxa2^+ encode putative proteases involved in the mating response." Mol.Cell.Biol.12. 1827-1834 (1992)
[Publications] N.Mochizuki: "Reduction in the intracellular cAMP level triggers initiation of sexual development in fission yeast." Mol.Gen.Genet.233. 17-24 (1992)
[Publications] T.Isshiki: "Characterization of a fission yeast gene,gpa2,that encode a G-alpha subunit involved in the monitoring of nutrition." Genes and Dev.6. 2455-2462 (1992)
[Publications] K.Tanaka: "Schizosaccharomyces pombe map3^+ encodes the putative Mfactor receptor." Mol.Cell.Biol.13. 80-88 (1993)

1992 Fiscal Year Annual Research Report

細胞複製制御の分子生物学的研究

Principal Investigator

岡崎 恒子 名古屋大学, 理学部, 教授 (10022584)

Research Products

[Publications] Y.Muro: "Centromere protein B assembles human centromeric α-satellite DNA at the 17-bp sequence,CENP-B box." J.Cell Biol.116. 585-596 (1992)

[Publications] K.Yoda: "A human centromere protein,CENP-B,has a DNA binding domain containing four potential α helices at the NH_2 terminus,which is separable from dimerizing activity." J.Cell Biol.119. 1413-1427 (1992)

[Publications] K.Shirahige: "Location and characterization of autonomously replicating sequences (ARSs) from chromosome VI of Saccharomyces cerevisiae." Mol.Cell.Biol.

[Publications] K.J.Begg: "Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutants." J.Bacteriol.174. 2416-2417 (1992)

[Publications] S.Hiraga: "Chromosome and plasmid partition in Escherichia coli." Ann.Rev.Biochem.61. 283-306 (1992)

[Publications] R.Imamura: "Characterization of high molecular weights of complexes and polymers of cytoplasmid proteins in Escherichia coli." Res.Microbiol.143. 743-753 (1992)

[Publications] K.Yamanaka: "Identification and characterization of the smbA gene,a suppressor of the muB null mutant of Escherichia coli." J.Bacteriol.174. 7517-7526 (1992)

[Publications] H.Niki: "E.coli MukB protein involved in chromosome partition forms a homodimer with a rod-and-hinge structure having DNA binding and ATP/GTP binding activities." EMBO J.11. 5101-5109 (1992)

[Publications] Y.Ishimi: "Topoisomerase II plays an essential role as a sivelase in the late stage of SV40 chromosome replication in vitro." J.Biol.Chem.267. 462-466 (1992)

[Publications] K.Sugasawa: "Non-conservative segregation of parental nucleosomes during simian virus 40 chromosome replication in vitro." Proc.Natl.Acad.Sci.USA. 89. 1055-1059 (1992)

[Publications] D.Ayusawa: "Complementation by a cloned human ubiquitin-activating enzyme El of the S-phase-arrested mouse FM3A cell mutant with thermolabile El." Cell Struct.Funct.17. 113-122 (1992)

[Publications] K.Ozawa: "Mapping of the human GSPTl gene,a human homolog of the yeast GST1 gene,to chromosomal band 16p13.1." Somat.cell Mol.Genet.18. 189-194 (1992)

[Publications] F.Yatagai: "Resolution and characterization of polymorphic DNA by SSCP and chemical cleavage methodologies." J.Rad.Res.33. 95-108 (1992)

[Publications] T.H.Norwood: "A Somatic genetic approach to the analysis of senescence in human diploid fibroblast in vitro:from heterokaryons to molecules." Exp.Gerontol.27. 391-395 (1992)

[Publications] H.Kamiya: "An abasic site analogue activates a c-Ha-ras gene by a point mutation at modified and adjacent positions." Nucleic Acids Res.20. 4409-4415 (1992)

[Publications] T.Sudo: "The c-myb proto-oncogene product binds to bu does not activate the promoter of the DNA polymerase α gene." Oncogene. 7. 1999-2006 (1992)

[Publications] N.Iwai: "Cloning and sequence of a functionally active cDNA for mouse ubiquitin-activating enzyme E." Gene. 118. 279-282 (1992)

[Publications] K.Segawa: "Abrogation of p53-mediated transactivation by SV40 large T antigen." Oncogene.

[Publications] H.Miyazawa: "Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase α-primase complex and their gene expression during cell proliferation and the cell cycle." J.Biol.Chem.268. (1993)

[Publications] K.Sugasawa: "Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts.I.Develpment of a cellfree system detecting excision repair of UV-irradiated SV40 chromosomes." J.Biol.Chem.268. (1993)

[Publications] C.Matsutani: "Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts.II.Defective DNA repair synthesis by xeroderma pigmentosum cell extracts." J.Biol.Chem.268. (1993)

[Publications] K.Matsumoto: "TAF-I,a novel host factor required to stimulate the adenovirus core DNA replication." J.Biol.Chem.268. (1993)

[Publications] Y.Imai: "S.pombe sxa1^+ and sxa2^+ encode putative proteases involved in the mating response." Mol.Cell.Biol.12. 1827-1834 (1992)

[Publications] N.Mochizuki: "Reduction in the intracellular cAMP level triggers initiation of sexual development in fission yeast." Mol.Gen.Genet.233. 17-24 (1992)

[Publications] T.Isshiki: "Characterization of a fission yeast gene,gpa2,that encode a G-alpha subunit involved in the monitoring of nutrition." Genes and Dev.6. 2455-2462 (1992)

[Publications] K.Tanaka: "Schizosaccharomyces pombe map3^+ encodes the putative Mfactor receptor." Mol.Cell.Biol.13. 80-88 (1993)

岡崎恒子名古屋大学, 理学部, 教授 (10022584)