1992 Fiscal Year Annual Research Report

蛋白質の膜透過と選別輸送の分子機構

Research Project

Project/Area Number	04259103
Research Institution	The University of Tokyo
Principal Investigator	水島昭二東京大学, 応用微生物研究所, 教授 (50013313)
Co-Investigator(Kenkyū-buntansha)	勝沼信彦徳島文理大学, 健康科学研究所, 教授 (50035375) 徳田元東京大学, 応用微生物研究所, 助教授 (40125943) 中野明彦東京大学, 理学部, 助教授 (90142140) 大村恒雄九州大学, 大学院医学系研究料, 教授 (80029933) 吉田匡山形大学, 医学部, 教授 (10004673)
Keywords	蛋白質の膜透過 / 蛋白質の選別輸送 / 総括班
Research Abstract	重点領域研究「蛋白質の膜透過と選別輸送の分子機構」の総括班として、研究計画の検討と研究成果の評価のため下記の活動を行った。 1.総括班会議を5月28日、10月9日、1月25日に開き、研究の進行状況および研究計画の検討を行った。この他、研究代表者と班長は随時連絡をとり、研究全般の円滑な進展をはかった。 2.1月25,26日の両日、山形で総括班と3つの研究計画班および公募採択研究の全研究者とその共同研究者合計約70名が出席し、各研究者による研究発法と出席者全員による討論がなされた。討論は極めて活発であった。 3.研究班の全体会議の他に、主として若手研究者(班員およびその共同研究者)による合宿形式の研究集会と8月19-21日に京都で開催した。約60名の参加があった。 4.全研究班員の連絡先と研究題目、研究経過と計画と記載した研究班員一覧をつくり、全研究班員に配布した。 5.本重点領域研究とほぼ同じ領域を取り扱う内藤記念科学振興財団の国際コンファレンス「細胞における蛋白質移行」の第2回が10月25日-28日に岐阜県川島町で開催され大多数の研究班員が参加し主導的役割を果した。海外から10名の第一線の一流研究者が参加し、本重点領域研究を推進させるのに大きく貢献した。

Research Products
(12 results)

All Other

All Publications (12 results)

[Publications] C.Hikita et al.: "The requirement of a positive charge at the amino terminus can be compensated for by a longercentral hydrophobic stretch in the functioning of signal peptides." J.Biol.Chem.267. 12375-12379 (1992)
[Publications] T.Suzuki et al.: "Evidence that rat liver mitochondrial and cytosolic fumarases are synthesized from one species of mRNA by alternative translation initiation at two in-phase AUG codons." Eur.J.Biochem.207. 767-772 (1992)
[Publications] M.Sakaguchi et al.: "Mitochondrial protein can be translocated across both endoplasmic reticulum and Mitochondrial mombranes." J.Biochem.112. 243-248 (1992)
[Publications] S.Nishikiwa et al.: "Identifiation of a novel gene required for membrane protein retention in the early secretory pathway." Proc.Natl.Acad.Sci.USA.
[Publications] K.Nishiyama et al.: "The carboxyl-terminal region of secE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli." J.Biol.Chem.267. 7170-7176 (1992)
[Publications] M.Mizutani et al.: "Molecular cloning and sequencing of a cDNA encoding mung bean cytochrome P450(P450C4H)possessing cinnamate 4-hydroxylase activity." Biochem.Biophys.Res.Commun.
[Publications] T.Satoh et al.: "Function of Ras as a molecular switch in signal transduction" J.Biol.Chem.267. 24149-24152 (1992)
[Publications] T.Suzuke et al.: "Evidence that rat liver mitochondrial and cytosolic fumarases are synthesized from one species of mRNA by alternative translocation initiation at two in-phase AUG codons." Eur.J.Biochem.207. 767-772 (1992)
[Publications] H.Shirataki et al.: "A possible target protein for smg-25a/rab3A small GTP-binding protein." J.Biol.Chem.267. 10946-10949 (1992)
[Publications] H.Takemoto et al.: "Heavy chain binding protein(BiP/GRP78)and endoplasmin are exported from the endoplasmic reticulum in rat exocrine Pancreatic cells,simlar to protein disulfide-isomerase." Arch.Biochem.Biophys.296. 129-136 (1992)
[Publications] Y.Nishimura et al.: "Expression of mouse cathepsin L cDNA in Saccharomyces cerevisiae:Evidence that cathepsin L is sirted for targeting to yeast vacuole." Arch.Biochem.Biophys.298. 318-324 (1992)
[Publications] M.Murata et al.: "pH-dependent membrane fusion and vesiculation of phospholipid large unilamellar vesicles induced by amphiphilic anionic and cationic peptide." Biochemistry. 31. 1986-1992 (1992)

1992 Fiscal Year Annual Research Report

蛋白質の膜透過と選別輸送の分子機構

Principal Investigator

水島 昭二 東京大学, 応用微生物研究所, 教授 (50013313)

Research Products

[Publications] C.Hikita et al.: "The requirement of a positive charge at the amino terminus can be compensated for by a longercentral hydrophobic stretch in the functioning of signal peptides." J.Biol.Chem.267. 12375-12379 (1992)

[Publications] T.Suzuki et al.: "Evidence that rat liver mitochondrial and cytosolic fumarases are synthesized from one species of mRNA by alternative translation initiation at two in-phase AUG codons." Eur.J.Biochem.207. 767-772 (1992)

[Publications] M.Sakaguchi et al.: "Mitochondrial protein can be translocated across both endoplasmic reticulum and Mitochondrial mombranes." J.Biochem.112. 243-248 (1992)

[Publications] S.Nishikiwa et al.: "Identifiation of a novel gene required for membrane protein retention in the early secretory pathway." Proc.Natl.Acad.Sci.USA.

[Publications] K.Nishiyama et al.: "The carboxyl-terminal region of secE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli." J.Biol.Chem.267. 7170-7176 (1992)

[Publications] M.Mizutani et al.: "Molecular cloning and sequencing of a cDNA encoding mung bean cytochrome P450(P450C4H)possessing cinnamate 4-hydroxylase activity." Biochem.Biophys.Res.Commun.

[Publications] T.Satoh et al.: "Function of Ras as a molecular switch in signal transduction" J.Biol.Chem.267. 24149-24152 (1992)

[Publications] T.Suzuke et al.: "Evidence that rat liver mitochondrial and cytosolic fumarases are synthesized from one species of mRNA by alternative translocation initiation at two in-phase AUG codons." Eur.J.Biochem.207. 767-772 (1992)

[Publications] H.Shirataki et al.: "A possible target protein for smg-25a/rab3A small GTP-binding protein." J.Biol.Chem.267. 10946-10949 (1992)

[Publications] H.Takemoto et al.: "Heavy chain binding protein(BiP/GRP78)and endoplasmin are exported from the endoplasmic reticulum in rat exocrine Pancreatic cells,simlar to protein disulfide-isomerase." Arch.Biochem.Biophys.296. 129-136 (1992)

[Publications] Y.Nishimura et al.: "Expression of mouse cathepsin L cDNA in Saccharomyces cerevisiae:Evidence that cathepsin L is sirted for targeting to yeast vacuole." Arch.Biochem.Biophys.298. 318-324 (1992)

[Publications] M.Murata et al.: "pH-dependent membrane fusion and vesiculation of phospholipid large unilamellar vesicles induced by amphiphilic anionic and cationic peptide." Biochemistry. 31. 1986-1992 (1992)

水島昭二東京大学, 応用微生物研究所, 教授 (50013313)