1993 Fiscal Year Annual Research Report

蛋白質の膜透過と選別輸送の分子機構

Research Project

Project/Area Number	04259103
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	水島昭二東京薬科大学, 生命科学部, 教授 (50013313)
Co-Investigator(Kenkyū-buntansha)	勝沼信彦徳島文理大学, 健康科学研究所, 教授 (50035375) 徳田元東京大学, 分子細胞生物学研究所, 助教授 (40125943) 中野明彦東京大学, 理学系研究科, 助教授 (90142140) 大村恒雄九州大学, 大学院医学系研究科, 教授 (80029933) 吉田匡山形大学, 医学部, 教授 (10004673)
Keywords	蛋白質の膜透過 / 蛋白質の分泌 / 小胞輸送 / 蛋白質の選別輸送 / 総括班
Research Abstract	重点領域研究「蛋白質の膜透過と選別輸送の分子機構」の総括班として、研究計画の検討と研究成果の評価のため下記の活動を行った。 1.総括班会議を5月18日、11月5日、12月14日に開き、研究の進行状況および研究計画の検討を行った。この他、研究代表者と班長は随時連絡をとり、研究全般の円滑な進展をはかった。尚、11月5日の会議には外国より招聘したこの分野の研究者3名も参加し、我が国の当該分野の研究に対するアドバイスを行った。 2.12月13、14日の両日、大阪で総括班と3つの研究計画班および公募採択研究の全研究者とその共同研究者合計約80名が出席し、各研究者による研究発表と出席者全員による討論がなされた。討論は極めて活発であった。 3.研究班の全体会議の他に、主として若手研究者(班員およびその共同研究者)による合宿形式の研究集会を7月27-29日に岐阜県中津川研修センターで開催した。70名を超す参加があった。 4.全研究班員の連絡先と研究題目、研究経過と計画を記載した研究班員一覧をつくり、全研究班員に配付した。 5.内藤記念科学振興財団と共催で国際コンファレンス「細胞における蛋白質移行」を11月2日-5日に岐阜県川島町で開催した。大多数の研究班員が参加し主導的役割を果した。海外から総括班で招聘した3名を含む17名の第一線の一流研究者が参加し、本重点領域研究を推進させるのに大きく貢献した。

Research Products

(21 results)

All Other

All Publications (21 results)

[Publications] S.Matsuyama et al.: "SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli." EMBO.J.12. 265-270 (1993)
[Publications] K.Nishiyama et al.: "A novel membrane protein involved in protein translocaiton across the cytoplasmic membrane of Escherichia coli." EMBO.J.12. 3409-3415 (1993)
[Publications] H.Ono et al.: "Transport of prepro‐albumin into inverted vesicles prepared from the inner membrane of rat liver mitochondria." FEBS Lett.318. 273-276 (1993)
[Publications] K.Takahashi et al.: "Integration of porin into the outer mitochondrial membrane through the common import machinery loccated in contact sites." Biochem.Nol.Biol.Internal.29. 429-434 (1993)
[Publications] N.Hachiya et al.: "Amitochondrial import factor purified from rat liver cytosol is an ATP‐dependent conformational modulator for precursor proteins." MEBO Journal. 12. 1579-1586 (1993)
[Publications] O.Shimozuwa et al.: "Core glycosylation of cytochrome P‐450(arom).Evidence for localization of Nterminus of microsomal P‐450 in the lumen." J.Biol Chem.268. 21399-21402 (1993)
[Publications] T.Kawabata.et al.: "Purification and processing of rat liver procathepsin B." J.Biochem.113. 389-394 (1993)
[Publications] M.Murata et al.: "Specificity of amphiphilic peptides for fusion of phospholi id vesicles." Biophys.J.64. 724-734 (1993)
[Publications] M.Murata et al.: "Fusion of dioleoylphosphatidylcholine vesicles induced by." Biochem.Biophys.Acta. 1152. 99-108 (1993)
[Publications] N.Henomatsu et al.: "Inhibition of intracellular transport of newly synthesized prolactin by bafilomycin Al in a pituitary tumor cell line,CH3 cells." Eur.J.Cell Biol.62. 127-139 (1993)
[Publications] S.Hoh et al.: "Immunoelectron microscopic localization of the HPC‐1 antigen in rat cerebellum." J.Neurocytology.22. 995-1005 (1993)
[Publications] S.Nishikawa et al.: "Identification of a gene required for membrane protein retention in theearly secretory pathway." Proc.Natl.Acad.Sci.U.S.A.90. 8179-8183 (1993)
[Publications] T.Oka et al.: "Inhibition of GTP hydrolysis by Sarlp causes accumulation of vesicles that are a functional intermediate of the ER‐to‐Golgi transport in yeast." J.Cell Biol.124(in press). (1994)
[Publications] S.Kawasaki et al.: "Membrane vasicles containing overproduced SecY and SecE exhibit high translocation ATPase activity and counter movement of protons in a SecA‐and Presecretory Protein‐dependent manner." J.Binl.Chem.268. 8193-8198 (1993)
[Publications] H.Koide et al.: "GTP‐dependent association of Raf‐1with Ha‐Ras:Identification of Raf as a terget downstream of Ras in mammalian cells." Proc.Natl.Acid.Sci.U.S.A.90. 8683-8686 (1993)
[Publications] M.Nakafuki et al.: "Suppression of oncogenic Ras by mutant neurofibromatosis type 1 genes with single amino acid substitutions." Proc.Natl.Acad.Sci.U.S.A.90. 6706-6710 (1993)
[Publications] Y.Matsunaga et al.: "Participation of cathepsin B in processing of antigen presentation to MHC class II." FEBS Lett.324. 325-330 (1993)
[Publications] M.Mizutani et al.: "Molecular cloning and sequencing of a cDNA encoding mung bean cytochromeP450(P450_<C4H>)possessing cinnamate 4‐hydroxylase activity." Biochem.Biophys.Res.Commun.190. 875-880 (1993)
[Publications] M.Mizutani et al.: "Purification and characterization of a cytochrome P450(trans‐cinnamate 4‐hydroxylase)frometiolated mung bean seedlings." Plant Cell Physiol.34. 481-488 (1993)
[Publications] S.Kishida et al.: "Rab3A GTPase‐activating protein‐inhibiting dctivity of Rab‐philin‐3A,a putative Rab3A tavgetprotein." J.Biol.Chem.268. 22259-22261 (1993)
[Publications] T.Yamaguchi et al.: "Two functionally different domains of rabphilin‐3A,Rab3A p25/smg p25A‐binding and phospnolipid‐and Ca^<2+>‐binding domains." J.Biol.Chem.268. 27164-27170 (1993)

1993 Fiscal Year Annual Research Report

蛋白質の膜透過と選別輸送の分子機構

Principal Investigator

水島 昭二 東京薬科大学, 生命科学部, 教授 (50013313)

Research Products

[Publications] S.Matsuyama et al.: "SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli." EMBO.J.12. 265-270 (1993)

[Publications] K.Nishiyama et al.: "A novel membrane protein involved in protein translocaiton across the cytoplasmic membrane of Escherichia coli." EMBO.J.12. 3409-3415 (1993)

[Publications] H.Ono et al.: "Transport of prepro‐albumin into inverted vesicles prepared from the inner membrane of rat liver mitochondria." FEBS Lett.318. 273-276 (1993)

[Publications] K.Takahashi et al.: "Integration of porin into the outer mitochondrial membrane through the common import machinery loccated in contact sites." Biochem.Nol.Biol.Internal.29. 429-434 (1993)

[Publications] N.Hachiya et al.: "Amitochondrial import factor purified from rat liver cytosol is an ATP‐dependent conformational modulator for precursor proteins." MEBO Journal. 12. 1579-1586 (1993)

[Publications] O.Shimozuwa et al.: "Core glycosylation of cytochrome P‐450(arom).Evidence for localization of Nterminus of microsomal P‐450 in the lumen." J.Biol Chem.268. 21399-21402 (1993)

[Publications] T.Kawabata.et al.: "Purification and processing of rat liver procathepsin B." J.Biochem.113. 389-394 (1993)

[Publications] M.Murata et al.: "Specificity of amphiphilic peptides for fusion of phospholi id vesicles." Biophys.J.64. 724-734 (1993)

[Publications] M.Murata et al.: "Fusion of dioleoylphosphatidylcholine vesicles induced by." Biochem.Biophys.Acta. 1152. 99-108 (1993)

[Publications] N.Henomatsu et al.: "Inhibition of intracellular transport of newly synthesized prolactin by bafilomycin Al in a pituitary tumor cell line,CH3 cells." Eur.J.Cell Biol.62. 127-139 (1993)

[Publications] S.Hoh et al.: "Immunoelectron microscopic localization of the HPC‐1 antigen in rat cerebellum." J.Neurocytology.22. 995-1005 (1993)

[Publications] S.Nishikawa et al.: "Identification of a gene required for membrane protein retention in theearly secretory pathway." Proc.Natl.Acad.Sci.U.S.A.90. 8179-8183 (1993)

[Publications] T.Oka et al.: "Inhibition of GTP hydrolysis by Sarlp causes accumulation of vesicles that are a functional intermediate of the ER‐to‐Golgi transport in yeast." J.Cell Biol.124(in press). (1994)

[Publications] S.Kawasaki et al.: "Membrane vasicles containing overproduced SecY and SecE exhibit high translocation ATPase activity and counter movement of protons in a SecA‐and Presecretory Protein‐dependent manner." J.Binl.Chem.268. 8193-8198 (1993)

[Publications] H.Koide et al.: "GTP‐dependent association of Raf‐1with Ha‐Ras:Identification of Raf as a terget downstream of Ras in mammalian cells." Proc.Natl.Acid.Sci.U.S.A.90. 8683-8686 (1993)

[Publications] M.Nakafuki et al.: "Suppression of oncogenic Ras by mutant neurofibromatosis type 1 genes with single amino acid substitutions." Proc.Natl.Acad.Sci.U.S.A.90. 6706-6710 (1993)

[Publications] Y.Matsunaga et al.: "Participation of cathepsin B in processing of antigen presentation to MHC class II." FEBS Lett.324. 325-330 (1993)

[Publications] M.Mizutani et al.: "Molecular cloning and sequencing of a cDNA encoding mung bean cytochromeP450(P450_<C4H>)possessing cinnamate 4‐hydroxylase activity." Biochem.Biophys.Res.Commun.190. 875-880 (1993)

[Publications] M.Mizutani et al.: "Purification and characterization of a cytochrome P450(trans‐cinnamate 4‐hydroxylase)frometiolated mung bean seedlings." Plant Cell Physiol.34. 481-488 (1993)

[Publications] S.Kishida et al.: "Rab3A GTPase‐activating protein‐inhibiting dctivity of Rab‐philin‐3A,a putative Rab3A tavgetprotein." J.Biol.Chem.268. 22259-22261 (1993)

[Publications] T.Yamaguchi et al.: "Two functionally different domains of rabphilin‐3A,Rab3A p25/smg p25A‐binding and phospnolipid‐and Ca^<2+>‐binding domains." J.Biol.Chem.268. 27164-27170 (1993)

水島昭二東京薬科大学, 生命科学部, 教授 (50013313)