1994 Fiscal Year Annual Research Report

蛋白質の膜透過と選別輸送の分子機構

Research Project

Project/Area Number	04259103
Research Institution	Tokyo University of Pharmacy and Life Science
Principal Investigator	水島昭二東京薬科大学, 生命科学部, 教授 (50013313)
Co-Investigator(Kenkyū-buntansha)	中野明彦東京大学, 大学院・理学系研究科, 助教授 (90142140) 徳田元東京大学, 分子細胞生物学研究所, 助教授 (40125943) 多賀谷光男東京薬科大学, 生命科学部, 助教授 (30179569) 吉田匡山形大学, 医学部, 教授 (10004673) 大村恒雄九州大学, 名誉教授 (80029933)
Keywords	蛋白質の膜透過 / 蛋白質の分泌 / 小胞輸送 / 蛋白質の選別輸送 / 総括班
Research Abstract	重点領域研究「蛋白質の膜透過と選別輸送の分子機構」の総括班として、研究計画の検討と研究成果の評価のため下記の活動を行った。 1.総括班会議を6月4日、9月9日、10月3日、12月6日に開き、研究の進行状況および研究計画の検討を行った。この他、研究代表者と班長は随時連絡をとり、研究全般の円滑な進展をはかった。尚、10月3日の会議には外国より招聘したこの分野の研究者3名も参加し、我が国の当該分野の研究に対するアドバイスを行った。 2.12月5、6日の両日,大阪で総括班と3つの研究計画班および公募採択研究の全研究者とその共同研究者合計80名が出席し、班会議を行った。各研究者による研究発表と出席者全員による討論がなされた。討論は極めて活発であった。 3.研究班の全体会議の他に、主として若手研究者(班員およびその共同研究者)による合宿形式の研究集会を7月25-27日に八王子市大学セミナーハウスで開催した。約80をの参加があった。 4.総括班主催で公開国際シンポジウム「Protein Targeting and Sorting in the Cell」を10月3日に東京都・東京ガ-デンパレスで開催した。大多数の研究班員が参加し主導的役割を果たした。海外からは総括班で招聘した3名と滞在中の1名の第一線の一流研究者が参加し、本重点領域研究を推進させるのに大きく貢献した。 5.中核的班員による懇談会を数回開催し、本重点領域研究の成果、問題点などについて討論し、当該分野の将来の発展の方向、そのための方策について検討した。 6.全研究班員の連絡先と研究題目、研究経過と計画を記載した研究班員一覧をつくり、全研究班員に配付し、研究交流に資した。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Sagara,K.: "SecF Stabrilizes SecD and SecY,Components of the Protein Translocation Machinery of the Escherichia coli Cytoplasmic Membrane." J.Bacteriol.176. 4111-4116 (1994)
[Publications] Hanada,M.: "Reconsitution of aneffidient ptoein translocation machinery comprising Sec-A and the three membrane proteins,SecY,SecE and SecG(p12)." J.Biol.Chem.269. 23625-25632 (1994)
[Publications] Dauville,K.: "The band 1 subunit of Escherichia coli prepreotein translocase and the integral membrane export factor P12 are the same protein." J.Biol.Chem.269. 18705-18707 (1994)
[Publications] Tohru Komiya: "Recognition of Mitochondria-targetion signals by a Cytosolic Import Stimulation Factor,MSF." Journal of Biological Chemistry. 269. 30893-30897 (1994)
[Publications] Naomi Hachiya: "MSF,a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria." EMBO Journal. 13. 5146-5154 (1994)
[Publications] M.Shirouzu: "Mutations that abolish the ability of Ha-Ras to associate with Raf-1" Oncogene. 9. 2153-2157 (1994)
[Publications] N.Katunuma: "MECHANISM AND REGULATION OF ANTIGEN PROCESSING BY CATHEPSIN B" Pergamon Press,Advan.Enzyme Regul. 34. 145-158 (1994)
[Publications] N.Katunuma: "Immunological significances of invariant chain from the aspect of its structural homology with the cystaitin family" FEBS Letters. 349. 265-269 (1994)
[Publications] M.Tkahashi: "Inhibition of growth and cysteine protease proteinase activity of Staphylococcus Aureus V8 phosphorylated cystain α in skin cornified envelope" FEBS Letters. 355. 275-278 (1994)
[Publications] T.Oka: "Inhibition of GTP hydrolysis by Sarlp causes accumulation of vesicles that are a functional intermediate of the ER-to-Golgitramport in yeast" J.Cell Biol.124. 425-434 (1994)
[Publications] A.Nakano: "Mutational analysis of the Sarl protein,a small GTPase which is essential for vesicular transport from endoplasmic reticulun" J.Biochem.116. 243-247 (1994)
[Publications] Nishiyama,K.: "Disruption of the gene encoding p12(SecG)reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature." EMBO J.13. 3272-3277 (1994)
[Publications] Hanada,M.: "Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins,SecY,SecE,and SecG(P12)." J.Biol.Chem.269. 23625-23631 (1994)
[Publications] M.Sumida: "Role of Two Nucleotide-binding Regions in an N-Ethylmaleimide-sensitive Factor Involved in Vesicle-mediated Protein Transport" J.Biol.Chem.269. 20636-20641 (1994)
[Publications] Rong-Mao Hong: "Association of N-Ethylmaleimide-sensitive Factor wich Synaptic Vesicles" FEBS Letters. 350. 253-257 (1994)
[Publications] T.Tsukamoto: "Peroxisome Assembly Factor-1:Nonsense Mutation in a Peroxisome-deficient Chinese Hamster Overy Cell Mutant and Deletion Analysis" Mol.Cell.Biol.14. 5458-5465 (1994)
[Publications] S.Miura: "Post-translational Import of 3-Ketoacyl-CoA Thiolase into Rat Liver Peroxisomes In Vitro." J.Biochem.115. 1064-1068 (1994)
[Publications] Y.Fujiki: "Peroxisomal Topogenic Signals and Etiology of Peroxisome-deficient Disease." Rothman,S.ed.(Membrane Protein Transport,Vol3)JAI Press,Inc.,

1994 Fiscal Year Annual Research Report

蛋白質の膜透過と選別輸送の分子機構

Principal Investigator

水島 昭二 東京薬科大学, 生命科学部, 教授 (50013313)

Research Products

[Publications] Sagara,K.: "SecF Stabrilizes SecD and SecY,Components of the Protein Translocation Machinery of the Escherichia coli Cytoplasmic Membrane." J.Bacteriol.176. 4111-4116 (1994)

[Publications] Hanada,M.: "Reconsitution of aneffidient ptoein translocation machinery comprising Sec-A and the three membrane proteins,SecY,SecE and SecG(p12)." J.Biol.Chem.269. 23625-25632 (1994)

[Publications] Dauville,K.: "The band 1 subunit of Escherichia coli prepreotein translocase and the integral membrane export factor P12 are the same protein." J.Biol.Chem.269. 18705-18707 (1994)

[Publications] Tohru Komiya: "Recognition of Mitochondria-targetion signals by a Cytosolic Import Stimulation Factor,MSF." Journal of Biological Chemistry. 269. 30893-30897 (1994)

[Publications] Naomi Hachiya: "MSF,a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria." EMBO Journal. 13. 5146-5154 (1994)

[Publications] M.Shirouzu: "Mutations that abolish the ability of Ha-Ras to associate with Raf-1" Oncogene. 9. 2153-2157 (1994)

[Publications] N.Katunuma: "MECHANISM AND REGULATION OF ANTIGEN PROCESSING BY CATHEPSIN B" Pergamon Press,Advan.Enzyme Regul. 34. 145-158 (1994)

[Publications] N.Katunuma: "Immunological significances of invariant chain from the aspect of its structural homology with the cystaitin family" FEBS Letters. 349. 265-269 (1994)

[Publications] M.Tkahashi: "Inhibition of growth and cysteine protease proteinase activity of Staphylococcus Aureus V8 phosphorylated cystain α in skin cornified envelope" FEBS Letters. 355. 275-278 (1994)

[Publications] T.Oka: "Inhibition of GTP hydrolysis by Sarlp causes accumulation of vesicles that are a functional intermediate of the ER-to-Golgitramport in yeast" J.Cell Biol.124. 425-434 (1994)

[Publications] A.Nakano: "Mutational analysis of the Sarl protein,a small GTPase which is essential for vesicular transport from endoplasmic reticulun" J.Biochem.116. 243-247 (1994)

[Publications] Nishiyama,K.: "Disruption of the gene encoding p12(SecG)reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature." EMBO J.13. 3272-3277 (1994)

[Publications] Hanada,M.: "Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins,SecY,SecE,and SecG(P12)." J.Biol.Chem.269. 23625-23631 (1994)

[Publications] M.Sumida: "Role of Two Nucleotide-binding Regions in an N-Ethylmaleimide-sensitive Factor Involved in Vesicle-mediated Protein Transport" J.Biol.Chem.269. 20636-20641 (1994)

[Publications] Rong-Mao Hong: "Association of N-Ethylmaleimide-sensitive Factor wich Synaptic Vesicles" FEBS Letters. 350. 253-257 (1994)

[Publications] T.Tsukamoto: "Peroxisome Assembly Factor-1:Nonsense Mutation in a Peroxisome-deficient Chinese Hamster Overy Cell Mutant and Deletion Analysis" Mol.Cell.Biol.14. 5458-5465 (1994)

[Publications] S.Miura: "Post-translational Import of 3-Ketoacyl-CoA Thiolase into Rat Liver Peroxisomes In Vitro." J.Biochem.115. 1064-1068 (1994)

[Publications] Y.Fujiki: "Peroxisomal Topogenic Signals and Etiology of Peroxisome-deficient Disease." Rothman,S.ed.(Membrane Protein Transport,Vol3)JAI Press,Inc.,

水島昭二東京薬科大学, 生命科学部, 教授 (50013313)