1992 Fiscal Year Annual Research Report

食品の生体防御因子の解析と設計

Research Project

Project/Area Number	04265104
Research Institution	Kyoto University
Principal Investigator	小清水弘一京都大学, 農学部, 教授 (90026518)
Co-Investigator(Kenkyū-buntansha)	山崎正利帝京大学, 薬学部, 教授 (00107393) 西野輔翼京都府立医科大学, 医学部, 助教授 (10079709) 多田富雄東京大学, 医学部, 教授 (10009136) 佐々木隆造京都大学, 農学部, 教授 (60077378) 池澤善郎横浜市立大学, 医学部, 助教授 (90046128)
Keywords	機能性食品 / アトピー性皮膚炎 / 免疫抑制 / 脂溶性ビタミン / リン脂質 / エリスロポエチンレセプター / 抗酸化剤 / 抗発癌プロモーター
Research Abstract	食品中の免疫および非免疫型生体防御因子に関する基礎的研究を行った。低アレルゲン米(HRS-1)のアトピー性皮膚炎への有効性が確認され、抗米塩不容性および可溶性IgE抗体価の比がHRS-1の有効性の指標となることを示した(池澤)。免疫細胞の沈静機能成分としてGinsengサポニンが一部で有効であることを明らかにした(多田)。一方、免疫系を刺激する成分としてチノイン酸やビタミンD_3を取り上げ、これらが腫瘍壊死因子(TNFα)、インターフェロンγ(INF-γ)、PGE_1(F_2α)と共同して、HL-60細胞を単球へと分化させることを示した(宇井)。リン脂質やスフィンゴシン(Sps)がINF-βの生産を増強することをin vitroおよびin vivo系で確認し、またその多くがマクロファージ貧食活性をも強めることを明らかにした(村上)。多くの食用植物はTNFの産生を誘導するが、その誘導に抑制的に働き、抗炎症、抗アレルギーにつながる植物(シリなど)もあることを確認した(山崎)。赤血球造血作用を持つ食品設計を目的とし、造血因子エリスロポエチンのレセプターの細胞外領域を動物細胞で生産する系を開発し、応用可能な段階にきた(佐々木)。ラットの肝細胞を用いた実験より、その細胞間接着に対して末端がラクトースの構造変化の重要性が示唆できた(佐内)。香辛料、特にショウガ科植物が生体の酸化的障害を顕著に抑制することを認めた(川岸)。熱帯産ショウガ科植物ナンキョウからは、強力な活性を持つ抗発癌プロモーター、1'-acetoxychavicol-acetateを単離同定した(小清水)。また、カロテノイドが抗発癌プロモーターとして有効なことを示した(西野)。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] H.OHIGASHI: "Possible anti-tumor promoting properties of marine algae and in vivo activity of wakame seaweed extract" Bioscience Biotechnology and Biochemistry. 56. 994-995 (1992)
[Publications] A.MURAKAMI: "Possible anti-tumor promoters;bi and tetraflavonoids from Lophira alata" Phytochemistry. 31. 2689-2693 (1992)
[Publications] Z.IKEZAWA: "Mass trial of hypoallergenic rice (HRS-1) produced by enzymatic digestion in Atopic dermatitis with suspected rice allergy" Acta Derm Venereol (Stockh) Suppl.176. 108-112 (1992)
[Publications] Z.IKEZAWA: "A possible involvement of rice allergy in severe type of Atopic dermatitis in Japan" Acta Derm Venereol (Stockh) Suppl.176. 103-107 (1992)
[Publications] M.SAIJO: "Growth state and cell cycle dependent phosphorylation of DNA topoisomerase II in Swiss 3T3 cells" Biochemistry. 31. 359-363 (1992)
[Publications] J.YANAGISAWA: "DNA-dependent adenosinetriphosphate Cl from mouse FM3A cells has DNA helicase activity" The Journal of Biological Chemistry. 267. 3644-3649 (1992)
[Publications] Y.MORIMITSU: "Inhibitors of platelet aggregation generated from mixtures of Allium species and/or S-alk(en)nyl-L-cysteine sulfoxides" Journal of Agricultural and Food Chemistry. 40. 368-372 (1992)
[Publications] Y.KATO: "Oxidative degradation of collagen and its model peptide by ultraviolet irradiation" Journal of Agricultural and Food Chemistry. 40. 373-379 (1992)
[Publications] M.OKANO: "Protein deficiency impairs erythropoiesis in rats by reducing serum erythropoietin concentration and the population size of erythroid precursor cells" Journal of Nutrition. 122. 1376-1383 (1992)
[Publications] K.HIDARI: "In vitro synthesis of disialosylganglioside from asialoglycolipid" Biochemistry. (1993)
[Publications] F.HU: "Establishment of stable CD8^+ suppressor T cell clones and the analysis of their suppressive function" Journal of Immunological Methods. 152. 123-134 (1992)
[Publications] Y.ASAKA: "5,7,3,4'-Tetrahydroxy-3-methoxy flavone,a potent anti-tumor promoter isolated from Gnaphalium indicum" Journal of Kyoto Prefecture University of Medicine. 101. 353-359 (1992)
[Publications] L.YU: "Potent anti-tumorigenic effect of tubeimoside 1 isolated from blub of Bolbostemma paniculatum (MAXIM) Franquet" International Journal of Cancer. 50. 635-638 (1992)
[Publications] T.TAHARA: "S timulation of interferon β production of cultured cells by phospholipids in foodstuffs" Bioscience Biotechnology and Biochemistry. 56. 1465-1466 (1992)
[Publications] M.YAMAZAKI: "Priming effects of vegetable juice on endogenous production of tumor necrosis factor" Bioscience Biotechnology and Biochemistry. 56. 149-149 (1992)
[Publications] M.YAMAZAKI: "Induction of neutrophil accumulation by vegetable juice" Bioscience Biotechnology and Biochenistry. 56. 150-151 (1992)

1992 Fiscal Year Annual Research Report

食品の生体防御因子の解析と設計

Principal Investigator

小清水 弘一 京都大学, 農学部, 教授 (90026518)

Research Products

[Publications] H.OHIGASHI: "Possible anti-tumor promoting properties of marine algae and in vivo activity of wakame seaweed extract" Bioscience Biotechnology and Biochemistry. 56. 994-995 (1992)

[Publications] A.MURAKAMI: "Possible anti-tumor promoters;bi and tetraflavonoids from Lophira alata" Phytochemistry. 31. 2689-2693 (1992)

[Publications] Z.IKEZAWA: "Mass trial of hypoallergenic rice (HRS-1) produced by enzymatic digestion in Atopic dermatitis with suspected rice allergy" Acta Derm Venereol (Stockh) Suppl.176. 108-112 (1992)

[Publications] Z.IKEZAWA: "A possible involvement of rice allergy in severe type of Atopic dermatitis in Japan" Acta Derm Venereol (Stockh) Suppl.176. 103-107 (1992)

[Publications] M.SAIJO: "Growth state and cell cycle dependent phosphorylation of DNA topoisomerase II in Swiss 3T3 cells" Biochemistry. 31. 359-363 (1992)

[Publications] J.YANAGISAWA: "DNA-dependent adenosinetriphosphate Cl from mouse FM3A cells has DNA helicase activity" The Journal of Biological Chemistry. 267. 3644-3649 (1992)

[Publications] Y.MORIMITSU: "Inhibitors of platelet aggregation generated from mixtures of Allium species and/or S-alk(en)nyl-L-cysteine sulfoxides" Journal of Agricultural and Food Chemistry. 40. 368-372 (1992)

[Publications] Y.KATO: "Oxidative degradation of collagen and its model peptide by ultraviolet irradiation" Journal of Agricultural and Food Chemistry. 40. 373-379 (1992)

[Publications] M.OKANO: "Protein deficiency impairs erythropoiesis in rats by reducing serum erythropoietin concentration and the population size of erythroid precursor cells" Journal of Nutrition. 122. 1376-1383 (1992)

[Publications] K.HIDARI: "In vitro synthesis of disialosylganglioside from asialoglycolipid" Biochemistry. (1993)

[Publications] F.HU: "Establishment of stable CD8^+ suppressor T cell clones and the analysis of their suppressive function" Journal of Immunological Methods. 152. 123-134 (1992)

[Publications] Y.ASAKA: "5,7,3,4'-Tetrahydroxy-3-methoxy flavone,a potent anti-tumor promoter isolated from Gnaphalium indicum" Journal of Kyoto Prefecture University of Medicine. 101. 353-359 (1992)

[Publications] L.YU: "Potent anti-tumorigenic effect of tubeimoside 1 isolated from blub of Bolbostemma paniculatum (MAXIM) Franquet" International Journal of Cancer. 50. 635-638 (1992)

[Publications] T.TAHARA: "S timulation of interferon β production of cultured cells by phospholipids in foodstuffs" Bioscience Biotechnology and Biochemistry. 56. 1465-1466 (1992)

[Publications] M.YAMAZAKI: "Priming effects of vegetable juice on endogenous production of tumor necrosis factor" Bioscience Biotechnology and Biochemistry. 56. 149-149 (1992)

[Publications] M.YAMAZAKI: "Induction of neutrophil accumulation by vegetable juice" Bioscience Biotechnology and Biochenistry. 56. 150-151 (1992)

小清水弘一京都大学, 農学部, 教授 (90026518)