1992 Fiscal Year Annual Research Report

細胞エネルギー共役系の構築と調節

Research Project

Project/Area Number	04266102
Research Institution	The University of Tokyo
Principal Investigator	安楽泰宏東京大学, 理学部, 教授 (20012643)
Co-Investigator(Kenkyū-buntansha)	向畑泰男名古屋大学, 理学部, 教授 (10028110) 川喜田正夫東京都臨床医学総合研究所, 医化学研究部, 部長 (00012740) 松原央大阪大学, 理学部, 教授 (00028242) 吉田賢右東京工業大学, 資源化学研究所, 教授 (90049073) 二井将光大阪大学, 産業科学研究所, 教授 (50012646)
Keywords	細胞エネルギー / ATP合成酵素 / 液胞型ATP分解酵素 / 呼吸酵素 / 電子伝達複合体 / 輸送蛋白 / オスモエンザイム / 化学浸透学説
Research Abstract	1.研究開始報告書および研究組織・班員班友名簿の作製:標記の資料を作製し、文部省および総括班員に提出配布した。2.ニュースレターの刊行:第1号(6月),第2号(9月),第3号(12月)を刊行し、重点領域研究の目的,計画,実施方法,国際交流の成果等を全班員に周知伝達した。3.運営委員会班長会審議の開催:10月に標記会議を開催し,平成5年度の研究組織計画を審議した。平成5年10月に「ATPase」,同12月に「呼繰酵素」に関するシンポジウムを開催することを決定した。4.平成5年度重点領域研究組織計画表の作製:標記の計画表を作製し(10月),文部省に提出した。5.総括班後援のシンポジウム「呼吸鎖電子伝達系膜複合体酸素:その立体構造と反応機構の最先端」の開催:標記シンポジウム(演者6名)を10月に開催し(福岡),計画班員,公募班員の最新成果を公開討議した。6.総括班主催のシンポジウム「生体膜のポンプと輸送」の開催:標記の公開シンポジウム(演者9名)を12月(東京)に開催し,計画班員,公募班員の最新成果を討議した。民間研究者も多数参会し盛況であった。7.総括班会議の開催:12月(東京)に開催し,平成4年度の経過報告,平成5年度の研究計画等を審議した。8.合同班会議の開催と研究成果の評価:合同班会議(12月17〜19日)を開催し、全班員の研究発表,成果の評価,平成5年度の実施計画,共同研究の推進,国際交流の活性化について討議した。

Research Products

(79 results)

All Other

All Publications (79 results)

[Publications] Y.Anraku: "Genetic and cell biological aspects of the yeast vacuolar H^+-ATPase." J.Bioenerg.Biomembr.24. 395-405 (1992)
[Publications] Y.Anraku: "Molecular genetics of the yeast vacuolar H^+-ATPase." J.Exp.Biol.172. 67-81 (1992)
[Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.1.Isolation anmd characterzation of two classes of vam mutants." J.Biol.Chem.267. 18665-18670 (1992)
[Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.2.VAM7,a gene for regulating morphogenic assembly of the vacuoes." J.Biol.Chem.267. 18671-18675 (1992)
[Publications] Y.Wada: "Chloride transport of yeast vacuolar membrane vesicles: A study of in vacuolar acidification." Biochim.Biophys.Acta. 1101. 296-302 (1992)
[Publications] R.Hirata: "Mutations at the putative junction sites of the yeast VMA1 protein,the catalytic subunit of the vacuoal membrane H^+-ATPase,inhibit its processing by protein splicing." Biochem.Biophys.Res.Commun.188. 40-47 (1992)
[Publications] T.Hisabori: "Single site hydrolysis of TNP-ATP by F_1-ATPase from the thermophilic bacterium PS3 is accelerated by chase-addition of excess ATP." J.Biol.Chem.267. 4551-4556 (1992)
[Publications] D.Bar-Zvi: "Covalent binding of 3'-0-(4-Benzoyl)benzoyl adenosine 5'-triphosphate (BzATP)to the isolated α and β subunits and α_3β_3 core complex of TF_1." J.Biol.Chem.267. 11029-11033 (1992)
[Publications] K.Tozawa: "Molecular dissection of the β subunit of F_1-ATPase into peptide fragments." J.Biol.Chem.267. 16484-16490 (1992)
[Publications] M.Sumi: "A DNA fragment homologous to F^1-ATPase β subunit was amplified from genomic DNA of Methanosarsina barkeri: Indication of an archaebacterial F-type ATPase." FEBS Lett.314. 207-210 (1992)
[Publications] N.Ishii.: "Three dimentional structure of F^1-ATPase of thermophilic bacterium PS3 obtained from electron crystallography." J.Biochem.113. 245-250 (1993)
[Publications] M,Jounouchi: "Catalytic site in the β subunit of H^+-ATPase from Escherichia coli:Glycine-rich sequence and its interaction weth Ser-174." Arch.Biochem.Biophys.292. 376-381 (1992)
[Publications] M,Jounouchi: "Role of the amino terminal region of the ε subunit of Escherichia coli H^+-ATPase(F_0F_1)." Arch.Biochem.Biophys.292. 87-94 (1992)
[Publications] M.Futai: "Catalytic site the β subunit of H^+-ATPase from Escherichia coli: Glycine-rich sequence and its interaction with Ser-174." Acta Physiol.Scan.146. 125-130 (1992)
[Publications] J.Miki: "Mapping of antigenic sites to monoclonal antibodies on the primary structure of the F_1-ATPase β subunit from Escherichia coli: Conserved amino-terminal region of the subunit in the F_1." Arch.Biochem.Biophys.294. 373-381 (1992)
[Publications] S.Noguchi: "Assembly of a hybrid from the α subunit Na^+/K^+-ATPase and β subunit of H^+/K^+-ATPase and β subunit of H^+/K^+-ATPase." Biochem.Biophys.Res.Commun.182. 659-666 (1992)
[Publications] M.Hasebe: "Vacuolar type H^+-ATPase genes:Presence of four genes including pseudogenes for the 16 kDa proteolipid subunit in the human genome." Biochem.Biophys.Res.Commyn.183. 659-666 (1992)
[Publications] S.Tamura: "Sequence motif in control region of the H^+/K^+-ATPase a and β subunit genes recognized by gastric specific nuclear protein." FEBS Lett.298. 137-141 (1992)
[Publications] Y.Moriyama: "Involvement of a non-proton pump factor (possibly Donnan-type equilibrium)in maintenance of an acidic pH in lysosomes." FEBS Lett.302. 18-20 (1992)
[Publications] M.Futai: "A glycine-rich sequence in the catalytic site of F-type ATPase." J.Biomembr.and Bioenerg.24. 463-467 (1992)
[Publications] Y.Moriyqma: "Role of vacuolar-type H^+-ATPase in neuronal and endocrine systems." J.Exp.Biol.172. 171-178 (1992)
[Publications] M.Futai: "Escherichia coli ATP synthase (F-type ATPase):Catalytic site and regulation of H^+ translocation." J.Exp.Biol.172. 443-449 (1992)
[Publications] H.Omote: "Effects mutations of conserved Lys-155 and Thr-156 residues in the phosphate binding glycine-rich sequence of the F_1-ATPase β subunit of Escherichia coli." J.Biol.Chem.267. 20571-20576 (1992)
[Publications] K.Shin: "F_0F_1-ATPase γ subunit mutations perturb the coupling between catalysis and transport" J.Biol.Chem.267. 20835-20839 (1992)
[Publications] R.K.Nakamoto: "Escherichia coli F_0F_1-ATPase:Residues involved in catalysis and coupling." Anal.N.Y.Acad.Sci.(1992)
[Publications] R.K.Nakamoto: "The γ subunit of the Escherichia coli ATP synthase:Mutations in the carboxyl-terminal region restore energy coupling to the amino-terminal mutant γ Met-23->Lys." Anal.N.Y.Acad.Sci.268. (1993)
[Publications] A.Iwamoto: "Domains near ATP γ phosphate in the catalytic site of H^+-ATPase:Model proposed from mutagenesis and inhibitor studies" J.Bl.N.Y.Acad.Sci.268. (1993)
[Publications] X.Wang: "Characterization of human autoantibodies reactibe to gastric parietal cells" Biochem.Biophys.Res.Commun.190. (1993)
[Publications] S.Yoshikawa: "Stategies for ctystallization of large membrane protein complexes." J.Crystal Growth. 122. 298-302 (1992)
[Publications] T.Yamashita: "Reconstitution of Na^+/H^+-antiporter of bovine renal brush-border membrane into proterliposomes and detection of a 110kDa protein cross- reactive with antibodies against a human Na^+/H^+-antiporter partial peptide in antiporter-active fractions after partial fractionation." J.Biochem.111. 162-167 (1992)
[Publications] M.Kuroda: "Immunohistochemical assessement of sarcoplasmic reticulum Ca^<2+>-ATPase distribution in various human tissues using novel monoclonal antibodies." Virchows Archiv A:Pathol. Anat.Histophthol.421. 527-532 (1992)
[Publications] T.Yamashita: "Characterization of Na^+/H^+-antipoter of bovine renal cortex and its immunoaffinity purification in reconstitutively actibe form." J.Biochem.113(2). (1993)
[Publications] Y.Kagawa: "The α_3β_3 and α_1β_1 complexes of ATP synthase." Ann.New York Acad.Sci.671. 366-376 (1992)
[Publications] H.Kamata: "Primary structure of the alanine carrier protein of thermophilic bacterium PS3." J.Biol.Chem.267. 21650-21655 (1992)
[Publications] Y.Kagawa: "The αβ complexes of ATP synthase:The αα_3β_3oligomer and α_1β_1 protomer." J.Bioenerg.Biomembr.24. 441-445 (1992)
[Publications] Y.Kobayashi: "The mutant mitochondrial genes in mitochondrial myopathy,encephalopathy,lactic acidosis and stroke-ike episodes (MELAS)were selectibely amplified through generations." J.Inher.Metab.Dis.15. 803-808 (1992)
[Publications] K.Tominaga: "Upstream region of a genomic gene for human mitochondrial transcription factor l." Biochim.Biophys.Acta. 1131. 217-219 (1992)
[Publications] H.Kamata: "Partial purification and reconstitution of inisitol 1,4,5-triphosphate receptor/Ca^2channel of bovine liver microsomes." J.Biochem.111. 546-552 (1992)
[Publications] C.Matsuda: "Immunohistochemical investigation of intracellular calcium pools in gerbil hippocampus following transient ischemia." Neuroscience Res.S17. S331 (1992)
[Publications] J.Masai: "Immaging of subunit complexes of thermophilic bacterium H^+-ATPase with scanning tunneling microscopy." UItramicroscopy. 42-44. 1194-1199 (1992)
[Publications] A.Ando: "Primary structure of chitosanase produced by Bacillus circulans MH-K." J.Gen.Appl.Microbiol.38. 135-144 (1992)
[Publications] Y.Kagawa: "Mitochondrial myopathy and transcriptional control." Med.Sports Sci.(Integration of Medical and Sports Science,Karger). 37. 324-328 (1992)
[Publications] S.Kawano: "Effect of proton pump inhibitor on gastric mucosa hemodynamics and tissue oxygenation in anesthetized rats." Eur.J.Pharmacol.211. 55-60 (1992)
[Publications] K.Shimazaki: "Involvement of calmodulin and calmodulin-dependent myosin light chain kinase in blue light-dependent H^+ pumping by guard cell protoplast from Vicia faba L" Plant Physiol.99(4). 1416-1421 (1992)
[Publications] K.Kusumi: "Accumulation of chloroplast tRNAGlu is temperature sensitive in virescent seedlings of rice." Plant Physiol.99(1). 142 (1992)
[Publications] H.Yamasaki: "Inhibition of the light-induced H^+ release from uncoupled thylakoid membrones by N-ethylmaleimide." Biochem.Biophys.Res.Commun.182. 1277-1281 (1992)
[Publications] I.Iwasaki: "Two types of channels involved in the malate ion transport across the tonoplast of a Crassulacean acid metabolism plant." Plant Physiol.98. 1494-1497 (1992)
[Publications] H.Arata: "Thermodynamics of malate transport across the tonoplast of leaf cells of CAM plants." Plant Cell Physiol.33. 873-880 (1992)
[Publications] K.Kusumi: "Temperature-sensitive expression of chloroplast tRNAGlu in virescent mutants of rice." Research in Photosynthesis(ed.N.Murata)Kluwer,Dordrecht. (1992)
[Publications] K.Kusumi: "Circadian rhythms in the regulation of phosphoenolpyruvate carbxylase from a CAM plant." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)
[Publications] I.Iwasaki: "Circadian rhythms in the regulation of phosphoenolpyruvate carbxylase from a CAM plant." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)
[Publications] T.Fuse: "Effects of light and high temperature on the leaves of a rice mutant sensitive to solar radiation." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)
[Publications] T.Kinoshita: "Dephosphorylation of the LHCII protein by light in guardcell protoplasts from Vicia faba L." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)
[Publications] H.Yamasaki: "Effect of catechols on the light-induced H^+ release from uncoupled membranes." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)
[Publications] K.Iba: "Chromosome walking in the region of Arabidopsis fadD locus using yeast artificial chromosomes." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)
[Publications] T.Matsuyama: "Leucine dehydrogenase from Bacillus stearothermophilus:Identification of active-site lysine by modification with pyridoxal phosphate." J.Biochem.112. 258-265 (1992)
[Publications] Y.Kazuta: "Probing the pyrophosphate-binding site in potato tuber UDP-glucose pyrophosphorylase with pyridoxal diphosphate." Protein Science. 2. (1993)
[Publications] H.Mori: "A chimeric α-glucan phosphorylase of plant type L and H isozymes.Functional role of 78-residue insertion in type L isozyme." J.Biol.Chem.268. (1993)
[Publications] T.Takada: "Quantitative immunogold localization of Na,K-ATPase along rat nephron." Histochemistry. 98. 183-197 (1992)
[Publications] K.Kawasaki: "Quantitative immunoelectron microscopic localization of Na,K-ATPase α-subunit in the epitherial cells of rat vestibular apparatus." Hear.Res.60. 64-72 (1992)
[Publications] T.Okami: "UItrastructural localization of Na^+,K^+-ATPase in the exorbital lacrimal gland of rat." Invest.Ophthamol.Vis,Sci.33. 196-204 (1992)
[Publications] H.Hayashi: "Properties of Active magnesium flux across the small intestine of guinea pig." Jpn.J.physiol.42. 561-575 (1992)
[Publications] T.Hoshi: "Proton secretion by the enterocytes and its role in intestinal absorptive functions." Adv.Physiol.Sci.(S.K.Manchanda,W.Selvamurthy,V.M.Kumar eds.)McMillan India,Delli,. 64-70 (1992)
[Publications] M.Nagasawa: "Purification of brush border membrane vesicles from rat renal cortex by size-exclusion chromatography." Anal.Biochem.201. 301-305 (1992)
[Publications] T.Goda: "Carbohydrate digestive capacity in jejunum of rats subjected to simulated weightlessness." J.Nutr.Biochem.3. 172-175 (1992)
[Publications] K.Ueda: "Human P-glycoprotein transports cortisol,aldosterone,and dexamethasone,but not progesterone." J.Biol.Chem.267. 24248-24252 (1992)
[Publications] H.Saito: "Transcellular transport of organic cation across monolayers of kidney epithelial cell line LLC-PK_1." Am.J.Physiol.262. C59-C66 (1992)
[Publications] R.Hori: "Expression of renal organic cation transporter in Xenopus leavis oocytes." Blochem.J.283(3. 409 (1992)
[Publications] R.Hori: "Surface binding and intracellular uptake of gentamicin in the cultured kidney epithelial cell line(LLC-PK_1)." J.Pharmacol.Exp.Ther.261(3. 1200-1205 (1992)
[Publications] M.Takano: "Transport of procainamide in a kidney epithelial cell line LLC-PK_1." Biochim.Biophys.Acta. 1108. 133-139 (1992)
[Publications] Y.Tanigawa: "Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLC-PK1)." J.Pharmacol.Exp.Ther.263(2. 840-845 (1992)
[Publications] K.Ohoka: "P-Aminohippurate transport in rat renal brush-border membranes:a potential-sensitive transport system and an anion exchanger." Biol.Pharm.Bull.
[Publications] T.Katsura: "Characteristics of organic cation transporter in rat renal basolateral membrane." Blochim.Biophys.Acta.
[Publications] Y.Mukuhata: "Photophosphorylation elements in Halobacteriaan A-type ATP synthase and bacterial rhodopsins." J.Bioenerg.Biomembr.24(6). 547-553 (1992)
[Publications] K.Ihara: "Halobacterial A-ATP synthase in relation to V-ATPase." J.Exp.Biol.172. 475-485 (1992)
[Publications] Y.Mukohata: "Comparative studies on bacteriorhodopsin-type pumps:Basic physicochemical data for proton pumps." Structures and Functions of Retinal Proteins (ed.J.L.Rigaud)Colloque INSERM,John Libbey Eurotext Ltd.,Montrouge,France. 221. PP101-104 (1992)
[Publications] J.Minagawa: "Identification of geme and copper ligands in subunit I of the cytochrome bo complex in Escherichia coli." J.Biol.Chem.267. 2096-2104 (1992)
[Publications] K.Saiki: "Hemo O biosynthesis in Escherichia coli:The cyoE gene in the cytocgrome bo operon encodes a protoheme IX farnesyltransferase." Biochim.Biophys.Res.Commun.189. 1491-1497 (1992)
[Publications] M.Tsubaki: "Structure of heme-copper binuclear center of the cytochrome bo complex in Escherichia coli:EPR and Fourier-transform infrared spectroscopic studies." Biochemistry. 32. (1993)

1992 Fiscal Year Annual Research Report

細胞エネルギー共役系の構築と調節

Principal Investigator

安楽 泰宏 東京大学, 理学部, 教授 (20012643)

Research Products

[Publications] Y.Anraku: "Genetic and cell biological aspects of the yeast vacuolar H^+-ATPase." J.Bioenerg.Biomembr.24. 395-405 (1992)

[Publications] Y.Anraku: "Molecular genetics of the yeast vacuolar H^+-ATPase." J.Exp.Biol.172. 67-81 (1992)

[Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.1.Isolation anmd characterzation of two classes of vam mutants." J.Biol.Chem.267. 18665-18670 (1992)

[Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.2.VAM7,a gene for regulating morphogenic assembly of the vacuoes." J.Biol.Chem.267. 18671-18675 (1992)

[Publications] Y.Wada: "Chloride transport of yeast vacuolar membrane vesicles: A study of in vacuolar acidification." Biochim.Biophys.Acta. 1101. 296-302 (1992)

[Publications] R.Hirata: "Mutations at the putative junction sites of the yeast VMA1 protein,the catalytic subunit of the vacuoal membrane H^+-ATPase,inhibit its processing by protein splicing." Biochem.Biophys.Res.Commun.188. 40-47 (1992)

[Publications] T.Hisabori: "Single site hydrolysis of TNP-ATP by F_1-ATPase from the thermophilic bacterium PS3 is accelerated by chase-addition of excess ATP." J.Biol.Chem.267. 4551-4556 (1992)

[Publications] D.Bar-Zvi: "Covalent binding of 3'-0-(4-Benzoyl)benzoyl adenosine 5'-triphosphate (BzATP)to the isolated α and β subunits and α_3β_3 core complex of TF_1." J.Biol.Chem.267. 11029-11033 (1992)

[Publications] K.Tozawa: "Molecular dissection of the β subunit of F_1-ATPase into peptide fragments." J.Biol.Chem.267. 16484-16490 (1992)

[Publications] M.Sumi: "A DNA fragment homologous to F^1-ATPase β subunit was amplified from genomic DNA of Methanosarsina barkeri: Indication of an archaebacterial F-type ATPase." FEBS Lett.314. 207-210 (1992)

[Publications] N.Ishii.: "Three dimentional structure of F^1-ATPase of thermophilic bacterium PS3 obtained from electron crystallography." J.Biochem.113. 245-250 (1993)

[Publications] M,Jounouchi: "Catalytic site in the β subunit of H^+-ATPase from Escherichia coli:Glycine-rich sequence and its interaction weth Ser-174." Arch.Biochem.Biophys.292. 376-381 (1992)

[Publications] M,Jounouchi: "Role of the amino terminal region of the ε subunit of Escherichia coli H^+-ATPase(F_0F_1)." Arch.Biochem.Biophys.292. 87-94 (1992)

[Publications] M.Futai: "Catalytic site the β subunit of H^+-ATPase from Escherichia coli: Glycine-rich sequence and its interaction with Ser-174." Acta Physiol.Scan.146. 125-130 (1992)

[Publications] J.Miki: "Mapping of antigenic sites to monoclonal antibodies on the primary structure of the F_1-ATPase β subunit from Escherichia coli: Conserved amino-terminal region of the subunit in the F_1." Arch.Biochem.Biophys.294. 373-381 (1992)

[Publications] S.Noguchi: "Assembly of a hybrid from the α subunit Na^+/K^+-ATPase and β subunit of H^+/K^+-ATPase and β subunit of H^+/K^+-ATPase." Biochem.Biophys.Res.Commun.182. 659-666 (1992)

[Publications] M.Hasebe: "Vacuolar type H^+-ATPase genes:Presence of four genes including pseudogenes for the 16 kDa proteolipid subunit in the human genome." Biochem.Biophys.Res.Commyn.183. 659-666 (1992)

[Publications] S.Tamura: "Sequence motif in control region of the H^+/K^+-ATPase a and β subunit genes recognized by gastric specific nuclear protein." FEBS Lett.298. 137-141 (1992)

[Publications] Y.Moriyama: "Involvement of a non-proton pump factor (possibly Donnan-type equilibrium)in maintenance of an acidic pH in lysosomes." FEBS Lett.302. 18-20 (1992)

[Publications] M.Futai: "A glycine-rich sequence in the catalytic site of F-type ATPase." J.Biomembr.and Bioenerg.24. 463-467 (1992)

[Publications] Y.Moriyqma: "Role of vacuolar-type H^+-ATPase in neuronal and endocrine systems." J.Exp.Biol.172. 171-178 (1992)

[Publications] M.Futai: "Escherichia coli ATP synthase (F-type ATPase):Catalytic site and regulation of H^+ translocation." J.Exp.Biol.172. 443-449 (1992)

[Publications] H.Omote: "Effects mutations of conserved Lys-155 and Thr-156 residues in the phosphate binding glycine-rich sequence of the F_1-ATPase β subunit of Escherichia coli." J.Biol.Chem.267. 20571-20576 (1992)

[Publications] K.Shin: "F_0F_1-ATPase γ subunit mutations perturb the coupling between catalysis and transport" J.Biol.Chem.267. 20835-20839 (1992)

[Publications] R.K.Nakamoto: "Escherichia coli F_0F_1-ATPase:Residues involved in catalysis and coupling." Anal.N.Y.Acad.Sci.(1992)

[Publications] R.K.Nakamoto: "The γ subunit of the Escherichia coli ATP synthase:Mutations in the carboxyl-terminal region restore energy coupling to the amino-terminal mutant γ Met-23->Lys." Anal.N.Y.Acad.Sci.268. (1993)

[Publications] A.Iwamoto: "Domains near ATP γ phosphate in the catalytic site of H^+-ATPase:Model proposed from mutagenesis and inhibitor studies" J.Bl.N.Y.Acad.Sci.268. (1993)

[Publications] X.Wang: "Characterization of human autoantibodies reactibe to gastric parietal cells" Biochem.Biophys.Res.Commun.190. (1993)

[Publications] S.Yoshikawa: "Stategies for ctystallization of large membrane protein complexes." J.Crystal Growth. 122. 298-302 (1992)

[Publications] M.Kuroda: "Immunohistochemical assessement of sarcoplasmic reticulum Ca^<2+>-ATPase distribution in various human tissues using novel monoclonal antibodies." Virchows Archiv A:Pathol. Anat.Histophthol.421. 527-532 (1992)

[Publications] T.Yamashita: "Characterization of Na^+/H^+-antipoter of bovine renal cortex and its immunoaffinity purification in reconstitutively actibe form." J.Biochem.113(2). (1993)

[Publications] Y.Kagawa: "The α_3β_3 and α_1β_1 complexes of ATP synthase." Ann.New York Acad.Sci.671. 366-376 (1992)

[Publications] H.Kamata: "Primary structure of the alanine carrier protein of thermophilic bacterium PS3." J.Biol.Chem.267. 21650-21655 (1992)

[Publications] Y.Kagawa: "The αβ complexes of ATP synthase:The αα_3β_3oligomer and α_1β_1 protomer." J.Bioenerg.Biomembr.24. 441-445 (1992)

[Publications] Y.Kobayashi: "The mutant mitochondrial genes in mitochondrial myopathy,encephalopathy,lactic acidosis and stroke-ike episodes (MELAS)were selectibely amplified through generations." J.Inher.Metab.Dis.15. 803-808 (1992)

[Publications] K.Tominaga: "Upstream region of a genomic gene for human mitochondrial transcription factor l." Biochim.Biophys.Acta. 1131. 217-219 (1992)

[Publications] H.Kamata: "Partial purification and reconstitution of inisitol 1,4,5-triphosphate receptor/Ca^2channel of bovine liver microsomes." J.Biochem.111. 546-552 (1992)

[Publications] C.Matsuda: "Immunohistochemical investigation of intracellular calcium pools in gerbil hippocampus following transient ischemia." Neuroscience Res.S17. S331 (1992)

[Publications] J.Masai: "Immaging of subunit complexes of thermophilic bacterium H^+-ATPase with scanning tunneling microscopy." UItramicroscopy. 42-44. 1194-1199 (1992)

[Publications] A.Ando: "Primary structure of chitosanase produced by Bacillus circulans MH-K." J.Gen.Appl.Microbiol.38. 135-144 (1992)

[Publications] Y.Kagawa: "Mitochondrial myopathy and transcriptional control." Med.Sports Sci.(Integration of Medical and Sports Science,Karger). 37. 324-328 (1992)

[Publications] S.Kawano: "Effect of proton pump inhibitor on gastric mucosa hemodynamics and tissue oxygenation in anesthetized rats." Eur.J.Pharmacol.211. 55-60 (1992)

[Publications] K.Shimazaki: "Involvement of calmodulin and calmodulin-dependent myosin light chain kinase in blue light-dependent H^+ pumping by guard cell protoplast from Vicia faba L" Plant Physiol.99(4). 1416-1421 (1992)

[Publications] K.Kusumi: "Accumulation of chloroplast tRNAGlu is temperature sensitive in virescent seedlings of rice." Plant Physiol.99(1). 142 (1992)

[Publications] H.Yamasaki: "Inhibition of the light-induced H^+ release from uncoupled thylakoid membrones by N-ethylmaleimide." Biochem.Biophys.Res.Commun.182. 1277-1281 (1992)

[Publications] I.Iwasaki: "Two types of channels involved in the malate ion transport across the tonoplast of a Crassulacean acid metabolism plant." Plant Physiol.98. 1494-1497 (1992)

[Publications] H.Arata: "Thermodynamics of malate transport across the tonoplast of leaf cells of CAM plants." Plant Cell Physiol.33. 873-880 (1992)

[Publications] K.Kusumi: "Temperature-sensitive expression of chloroplast tRNAGlu in virescent mutants of rice." Research in Photosynthesis(ed.N.Murata)Kluwer,Dordrecht. (1992)

[Publications] K.Kusumi: "Circadian rhythms in the regulation of phosphoenolpyruvate carbxylase from a CAM plant." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

[Publications] I.Iwasaki: "Circadian rhythms in the regulation of phosphoenolpyruvate carbxylase from a CAM plant." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

[Publications] T.Fuse: "Effects of light and high temperature on the leaves of a rice mutant sensitive to solar radiation." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

[Publications] T.Kinoshita: "Dephosphorylation of the LHCII protein by light in guardcell protoplasts from Vicia faba L." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

[Publications] H.Yamasaki: "Effect of catechols on the light-induced H^+ release from uncoupled membranes." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

[Publications] K.Iba: "Chromosome walking in the region of Arabidopsis fadD locus using yeast artificial chromosomes." Research in Photosynthesis (ed.N.Murata)Kluwer,Dodrecht. (1992)

[Publications] T.Matsuyama: "Leucine dehydrogenase from Bacillus stearothermophilus:Identification of active-site lysine by modification with pyridoxal phosphate." J.Biochem.112. 258-265 (1992)

[Publications] Y.Kazuta: "Probing the pyrophosphate-binding site in potato tuber UDP-glucose pyrophosphorylase with pyridoxal diphosphate." Protein Science. 2. (1993)

[Publications] H.Mori: "A chimeric α-glucan phosphorylase of plant type L and H isozymes.Functional role of 78-residue insertion in type L isozyme." J.Biol.Chem.268. (1993)

[Publications] T.Takada: "Quantitative immunogold localization of Na,K-ATPase along rat nephron." Histochemistry. 98. 183-197 (1992)

[Publications] K.Kawasaki: "Quantitative immunoelectron microscopic localization of Na,K-ATPase α-subunit in the epitherial cells of rat vestibular apparatus." Hear.Res.60. 64-72 (1992)

[Publications] T.Okami: "UItrastructural localization of Na^+,K^+-ATPase in the exorbital lacrimal gland of rat." Invest.Ophthamol.Vis,Sci.33. 196-204 (1992)

[Publications] H.Hayashi: "Properties of Active magnesium flux across the small intestine of guinea pig." Jpn.J.physiol.42. 561-575 (1992)

[Publications] T.Hoshi: "Proton secretion by the enterocytes and its role in intestinal absorptive functions." Adv.Physiol.Sci.(S.K.Manchanda,W.Selvamurthy,V.M.Kumar eds.)McMillan India,Delli,. 64-70 (1992)

[Publications] M.Nagasawa: "Purification of brush border membrane vesicles from rat renal cortex by size-exclusion chromatography." Anal.Biochem.201. 301-305 (1992)

[Publications] T.Goda: "Carbohydrate digestive capacity in jejunum of rats subjected to simulated weightlessness." J.Nutr.Biochem.3. 172-175 (1992)

[Publications] K.Ueda: "Human P-glycoprotein transports cortisol,aldosterone,and dexamethasone,but not progesterone." J.Biol.Chem.267. 24248-24252 (1992)

[Publications] H.Saito: "Transcellular transport of organic cation across monolayers of kidney epithelial cell line LLC-PK_1." Am.J.Physiol.262. C59-C66 (1992)

[Publications] R.Hori: "Expression of renal organic cation transporter in Xenopus leavis oocytes." Blochem.J.283(3. 409 (1992)

[Publications] R.Hori: "Surface binding and intracellular uptake of gentamicin in the cultured kidney epithelial cell line(LLC-PK_1)." J.Pharmacol.Exp.Ther.261(3. 1200-1205 (1992)

[Publications] M.Takano: "Transport of procainamide in a kidney epithelial cell line LLC-PK_1." Biochim.Biophys.Acta. 1108. 133-139 (1992)

[Publications] Y.Tanigawa: "Transport of digoxin by human P-glycoprotein expressed in a porcine kidney epithelial cell line (LLC-PK1)." J.Pharmacol.Exp.Ther.263(2. 840-845 (1992)

[Publications] K.Ohoka: "P-Aminohippurate transport in rat renal brush-border membranes:a potential-sensitive transport system and an anion exchanger." Biol.Pharm.Bull.

[Publications] T.Katsura: "Characteristics of organic cation transporter in rat renal basolateral membrane." Blochim.Biophys.Acta.

[Publications] Y.Mukuhata: "Photophosphorylation elements in Halobacteriaan A-type ATP synthase and bacterial rhodopsins." J.Bioenerg.Biomembr.24(6). 547-553 (1992)

[Publications] K.Ihara: "Halobacterial A-ATP synthase in relation to V-ATPase." J.Exp.Biol.172. 475-485 (1992)

[Publications] Y.Mukohata: "Comparative studies on bacteriorhodopsin-type pumps:Basic physicochemical data for proton pumps." Structures and Functions of Retinal Proteins (ed.J.L.Rigaud)Colloque INSERM,John Libbey Eurotext Ltd.,Montrouge,France. 221. PP101-104 (1992)

安楽泰宏東京大学, 理学部, 教授 (20012643)