1994 Fiscal Year Annual Research Report

細胞エネルギー共役系の構築と調節

Research Project

Project/Area Number	04266102
Research Institution	The University of Tokyo
Principal Investigator	安楽泰宏東京大学, 大学院・理学系研究科, 教授 (20012643)
Co-Investigator(Kenkyū-buntansha)	向畑泰男名古屋大学, 理学部, 教授 (10028110) 香川靖雄自治医科大学, 医学部, 教授 (30048962) 川喜田正夫東京都臨床医学総合研究所, (研究職), 部長 (00012740) 吉田賢右東京工業大学, 資源化学研究所, 教授 (90049073) 二井将光大阪大学, 産業科学研究所, 教授 (50012646)
Keywords	細胞エネルギー / ATP合成酵素 / 液胞型ATP分解酵素 / 呼吸酵素 / 電子伝達複合体 / 輸送タンパク / オスモエンザイム / 化学浸透光説
Research Abstract	本総括班の研究実績は以下のとおりである。 1.平成6年度の研究組織および研究者名簿、研究状況報告書を作成し、文部省に提出した(6年8月)。 2.ニュースレター第1号(6年7月)、第2号(7年2月)を刊行し重点領域研究の計画、実施状況、国際交流の成果等を全班員に周知伝達した。 3.運営委員会班長会議(6年9月)を開催し、公開講演会の開催、研究成果の取りまとめの方針を決定した。 4.班員の研究成果を速やかに公表するため、日本生化学会シンポジューム「細胞ホメオスタシスとイオン・溶質輸送」(6年9月)、日本分子生物学会シンポジューム「生体膜のエネルギー転換系の分子生物学」(6年12月)を開催した。 5.総括班主催の公開講演会「細胞イオンバランスの維持・調節」(6年12月:大阪千里ライフサイエンスセンター)を開催し、本重点領域研究の成果を公表した。 6.合同班会議(6年12月20-22日)を開催し、全班員の研究成果を討議した。総括班会議を併催して研究成果の合同評価を行い、研究業績の取りまとめの方針を決定した。 7.上記の方針に従い、平成7年化学研究費補助金「研究成果公開促進費」研究成果公開発表(A)計画調書を作成し、文部省に提出した(7年1月)。

Research Products

(87 results)

All Other

All Publications (87 results)

[Publications] Y.Mineuki: "Loss of microtubles in the interphase cells of onion (Allium cepa L.) root tips from the cell cortex and their appearance in the cytoplasm after treatment with cycloheximide." Plant Physiol.104. 281-284 (1994)
[Publications] J.Nakajima-Shimada: "Determination of intracellular Calcium concentration of individual host cells infected with Trypanosoma cruzi using microfluorimetry in conjunction with digital image processing." Jpn. J. Parasitol.43. 136-139 (1994)
[Publications] Y.Anraku: "Protozyme: Emerging evidence in nature." J. Biochem.115. 175-178 (1994)
[Publications] I.Yamato: "Site-specific alteration of Arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli." J. Biol. Chem.269. 5720-5724 (1994)
[Publications] S.Yoshida: "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae." Mol. Gen. Genet.242. 631-640 (1994)
[Publications] H.Qadota: "Conditional lethaliy of a yeast strain expressing human RHOA in place of RHO1." Proc. Natl. Acad. Sci. USA. 91. 9317-9321 (1994)
[Publications] Y.Takita: "The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca^<2+> tolerance in yeast." Mol. Gen. Genet.(in press.). (1995)
[Publications] Y.Moriyana: "ATP-dependent uptake anti-neoplastic agents by acidic organelles." J. Biochem.115. 213-218 (1994)
[Publications] T.Mogi: "Biosynthesis and functional role of heme O and heme A." Mol. Microbiol.14. 391-398 (1994)
[Publications] T.Mogi: "Molecular structure of a heme-copper redox center of the Escherichia coli ubiquinol oxidase: Evidence and model" J. Biochem.116. 741-747 (1994)
[Publications] M. Sato-Watanabe: "Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols." J. Biol. Chem.269. 28899-28907 (1994)
[Publications] M.Sato-Watanabe: "Identification of a novel quinone binding site in the cytochrome bo complex from Escherichia coli." J. Biol. Chem.269. 28908-28912 (1994)
[Publications] M.Tsubaki: "Molecular structure of redox metal centers of the cytochrome bo complex from Escherichia coli: Spectroscopic characterizations of the subunit I histidine mutant oxidases." J. Biol. Chem.(in press.). (1995)
[Publications] K.Saiki: "An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase." FEBS Lett.351. 385-388 (1994)
[Publications] S.Hirota: "Observation of the Fe-O_2 and Fe^<IV>=O Raman bands in dioxygen reduction by cytochrome bo-type ubiquinol oxidase from Escherichia coli." FEBS Lett.352. 67-70 (1994)
[Publications] Y.Orii: "Facilitated intra-molecular electron transfer in the cytochrome bo-type ubiquinol oxidase initiated upon reaction of the fully-reduced enzyme with dioxygen." FEBS Lett.352. 151-154 (1994)
[Publications] M.Maeda: "Gastric proton pump (H^+/K^+-ATPase): Structure and gene regulation through GATA DNA-binding protein(s)." J. Biochem.115. 6-14 (1994)
[Publications] H.Omote: "The α/β subunit interaction in H^+-ATPase (ATP synthase): An Eschericia coli α subunit mutation (αArg-296→Cys) restored coupling efficiency to the deleterious β subunit mutant (βSer-174→Phe)." J. Biol. Chem.269. 10265-10269 (1994)
[Publications] S.Mushiake: "Genes for members of the GATA-binding protein family (GATA-GT1 and GATA-GT2) together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)
[Publications] R.K.Nakamoto: "The F_oF_1 ATP synthase: Structure involved in catalysis, transport and coupling." Biomembranes. 6(in press.). (1995)
[Publications] M.Futai: "Catalysis and energy coupling ofH^+-ATPase(ATP synthase): Molecular biological approaches." Biochim. Biophys. Acta. 1187. 165-170 (1994)
[Publications] M.-Y. Park: "Conserved Glu-181 and Arg-182 residues of Escherichia coli H^+-ATPase (ATP synthase) β subunit are essential for catalysis: Properties of 33 mutants between βGlu-161 and βLys-201 residues." J. Biochem.116. 1139-1145 (1994)
[Publications] A.Iwamoto: "N-Ethlmaleimide-sensitive mutant (βVal-153→Cys) Escherichia coli F_1 ATPase: Cross-linking of the mutant β subunit with the α Subunit." FEBS Lett.352. 243-246 (1994)
[Publications] Y.Moriyama: "Quinnacrine mustard and hydrophobic amines both vacuolar H^+-ATPase and F_oF_1 ATP synthase." Biochim. Biophys. Res. Commun.(in press.). (1995)
[Publications] T.Uno: "Resonance Raman and Fourier-transform infrared studies on the subunit I histidine mutants of the cytochrome bo complex in Escherichia coli: Molecular structure of redox centers." J. Biol. Chem.269. 11912-11920 (1994)
[Publications] M.Futai: "H^+-ATPase: Catalysis and H^+ translocation." Molecular Aspects of Enzyme Catalysis. Kodansha 2nd/VCH Publishers, N.Y..(in press.). (1995)
[Publications] M.Yuzaki: "A stimulus paradigm inducing long-term desensitization of AMPA receptors evokes a specific increase in BDNF mRNA in cerebellar slices." Learning and Memory,. (in press.). (1995)
[Publications] S.Akiyama: "Energetics and formation of ATP synthase: Allosteric, chemiosmotic, and biosynthetic control of αβ and αβγ core." Biochemistry of Cell Membranes (Papa, S.and Tager, J.M.,eds.) Birkhauser, Basel, Switzerland,. (in press.).
[Publications] 二井将光: "オスモエンザイム, H^+-ATP合成酵素‥反応と輸送‥" 蛋白質核酸酵素. 39. 1141-1151 (1994)
[Publications] 香川靖雄: "パーティクル銃による遺伝子導入‥ミトコンドリア遺伝子治療とP^o株細胞" 実験医学増刊「遺伝子治療の最前線」. 12. 1835-1840 (1994)
[Publications] 香川靖雄: "インスリンと糖代謝の分子生物学." 病態生理. 13. 1-11 (1994)
[Publications] 香川靖雄: "ミトコンドリアの分子遺伝学と糖尿病." 日本臨床. 52. 85-91 (1994)
[Publications] M.Kawamoto: "New crystal forms and preliminary X-ray diffraction studies of mitochondrial cytochrome bc_1 complex from bovine heart." J.Mol.Biol.244. 238-241 (1994)
[Publications] T.Miyazaki: "Crystallization and preliminary X-ray diffraction studies of the α-amylase inhibitor coded 0.19 from wheat kernel." J.Biochem.115. 178-181 (1994)
[Publications] N.Kunishima: "Crystal structure of the fungal peroxidas from Arthromyces ramosus at 1.9Å resolution. Structural comparisons with the lignin and cytochrome c peroxidases." J.Mol.Biol.235. 331-344 (1994)
[Publications] J.-Y.Cui: "Molecular cloning and nucleotide sequences of cDNAs encoding subunits I, II, and IX of Euglena gracilis mitochondrial complex III." J.Biochem.115. 98-107 (1994)
[Publications] A.Ono: "Transport of envelope proteins of Sendai virus, HN and F_o, is blocked at different steps by thapsigargin and other perturbants to intracellular Ca^<2+>." J.Biochem.116. 649-656 (1994)
[Publications] T.Suzuki: "Sites of labeling with N-(3-pyrene)maleimide on Ca^<2+>-transporting ATPase of the sarcoplasmic reticulum." J.Biochem.117(in press.). (1995)
[Publications] J.-I. Hayashi: "Functional and morphological abnormalities of mitochondria in human cells containing mitochondrial DNA with pathogenic point mutations in tRNA genes." J.Biol.Chem.269. 19060-19066 (1994)
[Publications] J.-I. Hayashi: "Nuclear but not mitochondrial genome involvement in human age-related mitochondrial dysfunction. Functional integrity of mitochondrial DNA from aged subjects." J. Biol. Chem.269. 6878-6883 (1994)
[Publications] H.Endo: "Exclusion of an alternatively spliced exon in human ATP synthase γ-subunit pre-mRNA requires de novo protein synthesis." J.Biol.Chem.269. 12488-12493 (1994)
[Publications] S.Burgard: "Effects of magnesium ions on the relative conformation of nucleotide binding sites of F_1-ATPase as studied by ESR-spectroscopy." J.Biol.Chem.269. 17815-17819 (1994)
[Publications] M.Nakanishi: "Purification and reconstitution of an intestinal Na^+-dependent neutral L-α-amino acid transporter." J.Biol.Chem.269. 9325-9329 (1994)
[Publications] S.Akiyama: "Gene structure and cell type-specific expression of the human ATP α subunit." Biochim.Biophys.Acta. 1219. 129-140 (1994)
[Publications] A.Schuhen: "Photoaffinity cross-linking of ATP synthase complexes by fluorescent bifunctional diadenine-dinucleotide." Biol.Chem.Hoppe-Seyler. 375. S64 (1994)
[Publications] T.Koike: "Transcription of the MRP RNA gene in frog I oocytes requires a novel cis-element." Biochem.Biophys.Res.Commun.202. 225-233 (1994)
[Publications] M.Knoche: "2-Azido-1,N6-etheno-ATP: A fluorescent photoaffinity label for ATP synthase complexes." Biol.Chem.Hoppe Seyler. 375. S64 (1994)
[Publications] C.Matsuda: "Comparison of the ATPase activities of bovine heart and liver mitochondrial ATP synthase with different tissue-specific γ- subunit isoforms." Biochem.Biophys.Res.Commun.200. 671-678 (1994)
[Publications] 香川靖雄: "エネルギー代謝制御における糖質の意義." NANO GIGA. 3. 54-60 (1994)
[Publications] 香川靖雄: "パーティクル銃とP^O細胞の融合技術." 細胞工学. 13. 830-834 (1994)
[Publications] 富永薫: "核遺伝子によるミトコンドリアDNAの発現調節." 臨床科学. 130. 453-463 (1994)
[Publications] 香川靖雄: "分子シャペロンとその誘導." 蛋白質核酸酵素. 39. 808-817 (1994)
[Publications] 香川靖雄: "ミトコンドリア異常による糖尿病." Isotope News. 484. 5 (1994)
[Publications] 香川靖雄: "分子シャペロンとその誘導." 蛋白質核酸酵素. 39. 791-792 (1994)
[Publications] 香川靖雄: "ストレスタンパク質と細胞小器官." 細胞工学. 13. 169-175 (1994)
[Publications] S.Ueno: "Functional consequences of the substitution of disulfide-bonded segment, Cys127-Cys150, located in the extracellular domain of the Na, K-ATPase β subunit: Arg148 is essential for hte functional expression of Na, K-ATPase." J.Biochem.(in press.). (1995)
[Publications] K.Yokoyama: "Isolation of prokaryotic V_oV_1-ATPase from a thermophilic eubacterium Thermus thermophilus." J.Biol.Chem.269. 12248-12253 (1994)
[Publications] M.Okada: "Tyr-341 of the β subunit is a major Km-determining residue of TF_1-ATPase; Parallel effect of its mutations on Kd(ATP) of the β subunit and on the Km of the α_3β_3γ complex." J.Biochem.115. 789-796 (1994)
[Publications] K.Saika: "Crystallization of the mutant β subunit of F_1-ATPase from thermophilic Bacillus PS3." J.Mol.Biol.236. 709-711 (1994)
[Publications] T.Hisabori: "Asymmetry of the three catalytic sites on β subunits of TF_1 from a thermophilic Bacillus PS3." J.Biochem.115. 497-501 (1994)
[Publications] J.-M. Jault: "Probing the specificity of nucleotide binding to the F_1-ATPase from thermophiic Bacillus PS3 and its isolated α and β subunits with 2-N_3-[β,γ-^<32>P]ATP." Arch.Biochem.Biophis.310. 282-288 (1994)
[Publications] T.Amano: "Spatial precision of a catalytic carboxylate of F_1-ATPase β subunit probed by introducing different carboxylate containing side chains." FEBS Lett.348. 93-98 (1994)
[Publications] T.Amano: "Structural model of the ATP-binding domain of the F_1-β subunit based on analogy to the RecA protein." FEBS Lett.351. 1-5 (1994)
[Publications] S.Kawashima: "Widespread tissue distribution of multiple mitochondrial DNA deletions in familial mitochondrial myopathy." Muscle Nerve. 17. 741-746 (1994)
[Publications] Y.Kagawa: "Mitochondrial α-keto acid dehydrogenase complexes." Jp.Soc.Prom.Sci.,Tokyo. pp.1-37 (1994)
[Publications] Y.Tanaka: "Characterization of signaling pathways to Na^+/H^+ exchanger acivation with epidermal growth factor in hepatocytes." Hepatology. 20. 966-974 (1994)
[Publications] K.Kusumi: "Regulation of PEP-carboxylase by biological clock in a CAM plant." Plant Cell Physiol.35. 233-242 (1994)
[Publications] K.Kusumi: "Chlorophyll deficiency caused by a specific blockage of the C_5-pathway in seedlings of virescent mutant rice." Plant Cell Physiol.35. 445-449 (1994)
[Publications] H.Kodama: "Genetic enhancement of cold tolerance by expression of a gene for chloroplast ω-3 fatty acid desaturase in transgenic tobacco." Plant Physiol.105. 601-605 (1994)
[Publications] T.Hamada: "Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase." Gene. 147. 293-294 (1994)
[Publications] T.Nishiuchi: "Genomic nucleotide sequence of a gene encoding a microsomal ω-3 fatty acid desaturase from Arabidopsis thaliana." Plant Physiol.105. 767-768 (1994)
[Publications] R.M.Hong: "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles." FEBS Lett.350. 253-257 (1994)
[Publications] Y.Mukohata: "Comparative studies on ion pumps of the bacterial rhodopsin family." Biophys.Chem.50. 191-201 (1994)
[Publications] E.P.Lukashev: "pH dependence of the absorption spectra and photochemical transformations of the archaerhodopsins." Photochem.Photobiol.60. 69-75 (1994)
[Publications] Y.Sugiyama: "Archae-opsin expressed in Escherichia coli and its conversion to purple pigment." J.Biochem.115. 1021-1026 (1994)
[Publications] K.Ihara: "Met-145 is a key residue in the dark adaptation of bacteriorhodopsin homologs." Biophys.J.67. 1187-1191 (1994)
[Publications] M.Tateno: "The novel ion pump rhodopsin from Haloarcula form a family independent from both the bacteriorhodopsin and archaerhodopsin families/tribes." Arch.Biochem.Biophys.315. 127-132 (1994)
[Publications] Y.Sugiyama: "The light-driven proton pump, cruxrhodopsin-2 from Haloarcula sp.arg-2 (bR^+hR^-), and its coupled ATP formation." Biochim.Biophys.Acta. (in press.). (1995)
[Publications] Y.Orii: "Facilitated intramolecular electron transfer in the Escherichia coli bo-type ubiquinol oxidase requires chloride." Biochemistry. (in press.). (1995)
[Publications] K.Furukawa: "Identificatioh of Lys-277 at the active site of Escherichia coli glycogen synthase: Application of affinity labeling combined with site-directed mutagenesis." J.Biol.Chem.269. 868-871 (1994)
[Publications] S.Kaya: "Pyridoxal 5'-phosphate probes at Lys-480 can sense the binding of ATP and the formation of phosphoenzymes in Na^+,K^+-ATPase." J.Biol.Chem.269. 7419-7422 (1994)
[Publications] C.Hountondji: "Affinity labeling of the two species of Escherichia coli Lysyl-tRNA synthetase with Adenosine di- and triphosphopyridoxals." J.Biochem.116. 493-501 (1994)
[Publications] C.Hountondji: "Affinity labeling of the two species of Escherichia coli Lysyl-tRNA synthetase with pyridoxal mono- and diphosphophyridoxals." J.Biochem.116. 502-507 (1994)
[Publications] J.Deguchi: "Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium." Invest.Ophthalomol.Vis.Sci.35. 568-579 (1994)
[Publications] K.Sugasawa: "Immunocytochemical analyses of distribution of Na, K-ATPase and GLUTI, insulin and transferrin receptors in developing retinal pigment epithelial cells." Cell Struct.Funct.19. 21-28 (1994)
[Publications] G.Kawa: "Sodium pump distribution is not reversed in the DBA/2FG-pcy, polycystic kidney disease model mouse." J.Am.Soc.Nephrol.4. 2040-2049 (1994)
[Publications] A.Yamamoto: "Immunocytochemical localization of Na, K-ATPase in rat muscle spindles." Cell Struct.Funct.19. 179-187 (1994)

1994 Fiscal Year Annual Research Report

細胞エネルギー共役系の構築と調節

Principal Investigator

安楽 泰宏 東京大学, 大学院・理学系研究科, 教授 (20012643)

Research Products

[Publications] Y.Mineuki: "Loss of microtubles in the interphase cells of onion (Allium cepa L.) root tips from the cell cortex and their appearance in the cytoplasm after treatment with cycloheximide." Plant Physiol.104. 281-284 (1994)

[Publications] J.Nakajima-Shimada: "Determination of intracellular Calcium concentration of individual host cells infected with Trypanosoma cruzi using microfluorimetry in conjunction with digital image processing." Jpn. J. Parasitol.43. 136-139 (1994)

[Publications] Y.Anraku: "Protozyme: Emerging evidence in nature." J. Biochem.115. 175-178 (1994)

[Publications] I.Yamato: "Site-specific alteration of Arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli." J. Biol. Chem.269. 5720-5724 (1994)

[Publications] S.Yoshida: "Genetic interactions among genes involved in the STT4-PKC1 pathway of Saccharomyces cerevisiae." Mol. Gen. Genet.242. 631-640 (1994)

[Publications] H.Qadota: "Conditional lethaliy of a yeast strain expressing human RHOA in place of RHO1." Proc. Natl. Acad. Sci. USA. 91. 9317-9321 (1994)

[Publications] Y.Takita: "The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca^<2+> tolerance in yeast." Mol. Gen. Genet.(in press.). (1995)

[Publications] Y.Moriyana: "ATP-dependent uptake anti-neoplastic agents by acidic organelles." J. Biochem.115. 213-218 (1994)

[Publications] T.Mogi: "Biosynthesis and functional role of heme O and heme A." Mol. Microbiol.14. 391-398 (1994)

[Publications] T.Mogi: "Molecular structure of a heme-copper redox center of the Escherichia coli ubiquinol oxidase: Evidence and model" J. Biochem.116. 741-747 (1994)

[Publications] M. Sato-Watanabe: "Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols." J. Biol. Chem.269. 28899-28907 (1994)

[Publications] M.Sato-Watanabe: "Identification of a novel quinone binding site in the cytochrome bo complex from Escherichia coli." J. Biol. Chem.269. 28908-28912 (1994)

[Publications] M.Tsubaki: "Molecular structure of redox metal centers of the cytochrome bo complex from Escherichia coli: Spectroscopic characterizations of the subunit I histidine mutant oxidases." J. Biol. Chem.(in press.). (1995)

[Publications] K.Saiki: "An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase." FEBS Lett.351. 385-388 (1994)

[Publications] S.Hirota: "Observation of the Fe-O_2 and Fe^<IV>=O Raman bands in dioxygen reduction by cytochrome bo-type ubiquinol oxidase from Escherichia coli." FEBS Lett.352. 67-70 (1994)

[Publications] Y.Orii: "Facilitated intra-molecular electron transfer in the cytochrome bo-type ubiquinol oxidase initiated upon reaction of the fully-reduced enzyme with dioxygen." FEBS Lett.352. 151-154 (1994)

[Publications] M.Maeda: "Gastric proton pump (H^+/K^+-ATPase): Structure and gene regulation through GATA DNA-binding protein(s)." J. Biochem.115. 6-14 (1994)

[Publications] H.Omote: "The α/β subunit interaction in H^+-ATPase (ATP synthase): An Eschericia coli α subunit mutation (αArg-296→Cys) restored coupling efficiency to the deleterious β subunit mutant (βSer-174→Phe)." J. Biol. Chem.269. 10265-10269 (1994)

[Publications] S.Mushiake: "Genes for members of the GATA-binding protein family (GATA-GT1 and GATA-GT2) together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)

[Publications] R.K.Nakamoto: "The F_oF_1 ATP synthase: Structure involved in catalysis, transport and coupling." Biomembranes. 6(in press.). (1995)

[Publications] M.Futai: "Catalysis and energy coupling ofH^+-ATPase(ATP synthase): Molecular biological approaches." Biochim. Biophys. Acta. 1187. 165-170 (1994)

[Publications] M.-Y. Park: "Conserved Glu-181 and Arg-182 residues of Escherichia coli H^+-ATPase (ATP synthase) β subunit are essential for catalysis: Properties of 33 mutants between βGlu-161 and βLys-201 residues." J. Biochem.116. 1139-1145 (1994)

[Publications] A.Iwamoto: "N-Ethlmaleimide-sensitive mutant (βVal-153→Cys) Escherichia coli F_1 ATPase: Cross-linking of the mutant β subunit with the α Subunit." FEBS Lett.352. 243-246 (1994)

[Publications] Y.Moriyama: "Quinnacrine mustard and hydrophobic amines both vacuolar H^+-ATPase and F_oF_1 ATP synthase." Biochim. Biophys. Res. Commun.(in press.). (1995)

[Publications] T.Uno: "Resonance Raman and Fourier-transform infrared studies on the subunit I histidine mutants of the cytochrome bo complex in Escherichia coli: Molecular structure of redox centers." J. Biol. Chem.269. 11912-11920 (1994)

[Publications] M.Futai: "H^+-ATPase: Catalysis and H^+ translocation." Molecular Aspects of Enzyme Catalysis. Kodansha 2nd/VCH Publishers, N.Y..(in press.). (1995)

[Publications] M.Yuzaki: "A stimulus paradigm inducing long-term desensitization of AMPA receptors evokes a specific increase in BDNF mRNA in cerebellar slices." Learning and Memory,. (in press.). (1995)

[Publications] S.Akiyama: "Energetics and formation of ATP synthase: Allosteric, chemiosmotic, and biosynthetic control of αβ and αβγ core." Biochemistry of Cell Membranes (Papa, S.and Tager, J.M.,eds.) Birkhauser, Basel, Switzerland,. (in press.).

[Publications] 二井将光: "オスモエンザイム, H^+-ATP合成酵素‥反応と輸送‥" 蛋白質核酸酵素. 39. 1141-1151 (1994)

[Publications] 香川靖雄: "パーティクル銃による遺伝子導入‥ミトコンドリア遺伝子治療とP^o株細胞" 実験医学増刊「遺伝子治療の最前線」. 12. 1835-1840 (1994)

[Publications] 香川靖雄: "インスリンと糖代謝の分子生物学." 病態生理. 13. 1-11 (1994)

[Publications] 香川靖雄: "ミトコンドリアの分子遺伝学と糖尿病." 日本臨床. 52. 85-91 (1994)

[Publications] M.Kawamoto: "New crystal forms and preliminary X-ray diffraction studies of mitochondrial cytochrome bc_1 complex from bovine heart." J.Mol.Biol.244. 238-241 (1994)

[Publications] T.Miyazaki: "Crystallization and preliminary X-ray diffraction studies of the α-amylase inhibitor coded 0.19 from wheat kernel." J.Biochem.115. 178-181 (1994)

[Publications] N.Kunishima: "Crystal structure of the fungal peroxidas from Arthromyces ramosus at 1.9Å resolution. Structural comparisons with the lignin and cytochrome c peroxidases." J.Mol.Biol.235. 331-344 (1994)

[Publications] J.-Y.Cui: "Molecular cloning and nucleotide sequences of cDNAs encoding subunits I, II, and IX of Euglena gracilis mitochondrial complex III." J.Biochem.115. 98-107 (1994)

[Publications] A.Ono: "Transport of envelope proteins of Sendai virus, HN and F_o, is blocked at different steps by thapsigargin and other perturbants to intracellular Ca^<2+>." J.Biochem.116. 649-656 (1994)

[Publications] T.Suzuki: "Sites of labeling with N-(3-pyrene)maleimide on Ca^<2+>-transporting ATPase of the sarcoplasmic reticulum." J.Biochem.117(in press.). (1995)

[Publications] J.-I. Hayashi: "Functional and morphological abnormalities of mitochondria in human cells containing mitochondrial DNA with pathogenic point mutations in tRNA genes." J.Biol.Chem.269. 19060-19066 (1994)

[Publications] J.-I. Hayashi: "Nuclear but not mitochondrial genome involvement in human age-related mitochondrial dysfunction. Functional integrity of mitochondrial DNA from aged subjects." J. Biol. Chem.269. 6878-6883 (1994)

[Publications] H.Endo: "Exclusion of an alternatively spliced exon in human ATP synthase γ-subunit pre-mRNA requires de novo protein synthesis." J.Biol.Chem.269. 12488-12493 (1994)

[Publications] S.Burgard: "Effects of magnesium ions on the relative conformation of nucleotide binding sites of F_1-ATPase as studied by ESR-spectroscopy." J.Biol.Chem.269. 17815-17819 (1994)

[Publications] M.Nakanishi: "Purification and reconstitution of an intestinal Na^+-dependent neutral L-α-amino acid transporter." J.Biol.Chem.269. 9325-9329 (1994)

[Publications] S.Akiyama: "Gene structure and cell type-specific expression of the human ATP α subunit." Biochim.Biophys.Acta. 1219. 129-140 (1994)

[Publications] A.Schuhen: "Photoaffinity cross-linking of ATP synthase complexes by fluorescent bifunctional diadenine-dinucleotide." Biol.Chem.Hoppe-Seyler. 375. S64 (1994)

[Publications] T.Koike: "Transcription of the MRP RNA gene in frog I oocytes requires a novel cis-element." Biochem.Biophys.Res.Commun.202. 225-233 (1994)

[Publications] M.Knoche: "2-Azido-1,N6-etheno-ATP: A fluorescent photoaffinity label for ATP synthase complexes." Biol.Chem.Hoppe Seyler. 375. S64 (1994)

[Publications] C.Matsuda: "Comparison of the ATPase activities of bovine heart and liver mitochondrial ATP synthase with different tissue-specific γ- subunit isoforms." Biochem.Biophys.Res.Commun.200. 671-678 (1994)

[Publications] 香川靖雄: "エネルギー代謝制御における糖質の意義." NANO GIGA. 3. 54-60 (1994)

[Publications] 香川靖雄: "パーティクル銃とP^O細胞の融合技術." 細胞工学. 13. 830-834 (1994)

[Publications] 富永薫: "核遺伝子によるミトコンドリアDNAの発現調節." 臨床科学. 130. 453-463 (1994)

[Publications] 香川靖雄: "分子シャペロンとその誘導." 蛋白質核酸酵素. 39. 808-817 (1994)

[Publications] 香川靖雄: "ミトコンドリア異常による糖尿病." Isotope News. 484. 5 (1994)

[Publications] 香川靖雄: "分子シャペロンとその誘導." 蛋白質核酸酵素. 39. 791-792 (1994)

[Publications] 香川靖雄: "ストレスタンパク質と細胞小器官." 細胞工学. 13. 169-175 (1994)

[Publications] S.Ueno: "Functional consequences of the substitution of disulfide-bonded segment, Cys127-Cys150, located in the extracellular domain of the Na, K-ATPase β subunit: Arg148 is essential for hte functional expression of Na, K-ATPase." J.Biochem.(in press.). (1995)

[Publications] K.Yokoyama: "Isolation of prokaryotic V_oV_1-ATPase from a thermophilic eubacterium Thermus thermophilus." J.Biol.Chem.269. 12248-12253 (1994)

[Publications] M.Okada: "Tyr-341 of the β subunit is a major Km-determining residue of TF_1-ATPase; Parallel effect of its mutations on Kd(ATP) of the β subunit and on the Km of the α_3β_3γ complex." J.Biochem.115. 789-796 (1994)

[Publications] K.Saika: "Crystallization of the mutant β subunit of F_1-ATPase from thermophilic Bacillus PS3." J.Mol.Biol.236. 709-711 (1994)

[Publications] T.Hisabori: "Asymmetry of the three catalytic sites on β subunits of TF_1 from a thermophilic Bacillus PS3." J.Biochem.115. 497-501 (1994)

[Publications] J.-M. Jault: "Probing the specificity of nucleotide binding to the F_1-ATPase from thermophiic Bacillus PS3 and its isolated α and β subunits with 2-N_3-[β,γ-^<32>P]ATP." Arch.Biochem.Biophis.310. 282-288 (1994)

[Publications] T.Amano: "Spatial precision of a catalytic carboxylate of F_1-ATPase β subunit probed by introducing different carboxylate containing side chains." FEBS Lett.348. 93-98 (1994)

[Publications] T.Amano: "Structural model of the ATP-binding domain of the F_1-β subunit based on analogy to the RecA protein." FEBS Lett.351. 1-5 (1994)

[Publications] S.Kawashima: "Widespread tissue distribution of multiple mitochondrial DNA deletions in familial mitochondrial myopathy." Muscle Nerve. 17. 741-746 (1994)

[Publications] Y.Kagawa: "Mitochondrial α-keto acid dehydrogenase complexes." Jp.Soc.Prom.Sci.,Tokyo. pp.1-37 (1994)

[Publications] Y.Tanaka: "Characterization of signaling pathways to Na^+/H^+ exchanger acivation with epidermal growth factor in hepatocytes." Hepatology. 20. 966-974 (1994)

[Publications] K.Kusumi: "Regulation of PEP-carboxylase by biological clock in a CAM plant." Plant Cell Physiol.35. 233-242 (1994)

[Publications] K.Kusumi: "Chlorophyll deficiency caused by a specific blockage of the C_5-pathway in seedlings of virescent mutant rice." Plant Cell Physiol.35. 445-449 (1994)

[Publications] H.Kodama: "Genetic enhancement of cold tolerance by expression of a gene for chloroplast ω-3 fatty acid desaturase in transgenic tobacco." Plant Physiol.105. 601-605 (1994)

[Publications] T.Hamada: "Cloning of a cDNA encoding tobacco ω-3 fatty acid desaturase." Gene. 147. 293-294 (1994)

[Publications] T.Nishiuchi: "Genomic nucleotide sequence of a gene encoding a microsomal ω-3 fatty acid desaturase from Arabidopsis thaliana." Plant Physiol.105. 767-768 (1994)

[Publications] R.M.Hong: "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles." FEBS Lett.350. 253-257 (1994)

[Publications] Y.Mukohata: "Comparative studies on ion pumps of the bacterial rhodopsin family." Biophys.Chem.50. 191-201 (1994)

[Publications] E.P.Lukashev: "pH dependence of the absorption spectra and photochemical transformations of the archaerhodopsins." Photochem.Photobiol.60. 69-75 (1994)

[Publications] Y.Sugiyama: "Archae-opsin expressed in Escherichia coli and its conversion to purple pigment." J.Biochem.115. 1021-1026 (1994)

安楽泰宏東京大学, 大学院・理学系研究科, 教授 (20012643)