細胞エネルギー共役系の構築と調節

1995 Fiscal Year Annual Research Report

• Project/Area Number: 04266102
• Research Institution: The University of Tokyo
• Principal Investigator: 安楽 泰宏 東京大学, 大学院・理学系研究科, 教授 (20012643)
• Co-Investigator(Kenkyū-buntansha): 向畑 泰男 名古屋大学, 理学部, 教授 (10028110) ; 香川 靖雄 自治医科大学, 医学部, 教授 (30048962) ; 川喜田 正夫 東京都臨床医学総合研究所, 部長 (00012740) ; 吉田 賢右 東京工業大学, 資源化学研究所, 教授 (90049073) ; 二井 将光 大阪大学, 産業科学研究所, 教授 (50012646)
• Keywords: 細胞エネルギー / ATP合成酵素 / 液胞型ATP分解酵素 / 呼吸酵素 / 電子伝達複合体 / 輸送タンパク / オスモエンザイム / 化学浸透学説

Research Abstract

本総括班の研究実績は以下のとおりである.
1.平成7年10月,「細胞エネルギー共役系の構築と調節」(平成4年度-6年度)に係わる研究経過等の報告書をとりまとめ,同年10月の業績・成果報告会に提出し,ヒアリングを受けた.
2.運営委員会(7年9月17日および11月14-15日)を開催し,成果とりまとめの基本方針を審議した.
3.総括班主催の公開講演会「バイオエナジェテイクスの新展開:エネルギー変換系の構造とメカニクス」(7年12月19日:名古屋大学医学部・鶴友会館)を開催し,本重点領域研究の成果を発表するとともに,今後の研究推進方針を討議した.
4.合同班会議(6年12月20-22日)を開催し,全班員の研究成果を討議し,その評価を行った.
5.総括班会議を併催して研究成果の合同評価を行った.また,研究実績報告書の取りまとめの方針を決定し,同報告書を平成8年2月上梓した.

Research Products

• [Publications] I.Tanida: "Cooperation of calcium and vacuolar H^+-ATPase in intracellular Ca^<2+>homeostasis of yest cells." J.Biol.Chem.270. 10113-10119 (1995)
• [Publications] M.Maeda: "Roles of gastric GATA DNA-binding proteins." J.Exp.Biol.(In press). (1996)
• [Publications] H.Omote: "β Subunit Glu-185 of Escheirchia coliH^+-ATPase(ATP synthase)is an esential residue for cooperative catalysis." J.Biol.Chem.270. 25656-25660 (1995)
• [Publications] C.Jeanteur: "β/α Subunit interaction is required for catalysis by H^+-ATPase(ATP synthase):β Subunit amino terminal replacements suppress a γ subunit mutation having long unrelated carboxyl terminal." J.Biol.Chem.270. 22850-22854 (1995)
• [Publications] R.K.Nakamoto: "The ATP synthase γ subunit:Suppresor mutagenesis reveals three helical regions involved in energy coupling." J.Biol.Chem.270. 14042-14046 (1995)
• [Publications] Y.Moriyama: "Microvesicles isolated from bovine posterior pituitary accumulate norepinephrine." J.Biol.Chem.270. 11424-11429 (1995)
• [Publications] Y.Moriyama: "Quinacrine mustard and lipophilic cations inhibitory to both vacuolar H^+-ATPase and F_0_1-ATP synthase." FEBS Lett. 359. 69-72 (1995)
• [Publications] M.Maeda: "The intrinsic factor gene:Its 5′-upstream region and chief cell-specific trnscription." J.Biochem.117. 1305-1311 (1995)
• [Publications] T.Nishi: "Identification of the promoter region of the human histamine H2 receptorgene." Biochem.Biophys.Res.Commum.210. 616-623 (1995)
• [Publications] M.Yoshida: "A common topology of proteins catalyzing ATP-triggered reactions." FEBS Lett.359. 1-5 (1995)
• [Publications] M.Miyauchi: "F_1ATPase α-subunit made up from two fragments(1-395,396-503)is stabilized by ATP and complexes containing it obey altered kinetics." Biochim.Biophys.Acta. 1229. 225-232 (1995)
• [Publications] K.Saika: "Aminimum catalytic unit of F_1-ATPase shows non-cooperative ATPase activity inherent in a single catalytic site with a K_m70μM." FEBS Lett.368. 207-210 (1995)
• [Publications] T.Matsui: "Expression of the wild-type andthe Cys-/Trp-less α3β3γ complex of thermipholic F_1-ATPase in Escherichia coli." Biochim.Biohys.Acta. 1231. 139-146 (1995)
• [Publications] T.Kato: "Analysis of time dependent change of Escherichia ColiF_1-ATPase activity and its relationship with apparent negative cooperativity." Biochim.Biophys.Acta. 1231. 275-281 (1995)
• [Publications] K.Tozawa: "Conformational dynamics monitored by His-179 and His-200 of isolated thermophilic F_1-ATPase β subunit which rerside at the entrance of′conical tunnel′ in holoenzyme." FEBS Lett.376. 190-194 (1995)
• [Publications] C.Kaibara: "Strutural asymmetry of F_1-ATPase caused by the γ subunit generates a high affinity nucleotide binding site" J.Biol.Chem.271(印刷中). (1996)
• [Publications] K.Fukuyama: "Tertiary structure of [2Fe-2S]ferredoxin from Spirulina platensis refined at 2.5Å resolution:Structural comparison of plant-type ferredoxins and electrostatic potential analysis." J.Biochem.117. 1017-1023 (1995)
• [Publications] H.Oh-oka: "Two molecules of cytochrome c function as the electron donors to P840 in the reaction center complex isolated from a green bacterium,Chlorobium tepium." FEBS Lett.365. 30-34 (1995)
• [Publications] H.oh-oka: "Photoactive reaction center(PscA/cytochrome c_<551>)_2complex of Chlorobium limicola." Proc.Xth Int.Photosynthesis Congress. (印刷中). (1995)
• [Publications] 南 善子: "広く生体反応に関わる鉄硫黄蛋白質" 生物物理. 35. 7-12 (1995)
• [Publications] A.Ono: "Conformational aberrance of Sendai virus F_0 protein in thapsigargin-treated cells allowing exit from the endoplasmic reticulum but causing arrest at the Golgi complex." J.Biochem.118. 1248-1254 (1995)
• [Publications] M.Sugimoto: "Significance of urinary N1,N8-diacetylspermidine and N1,N12-diacetylspermine as indicators of neoplastic diseases." J.Cancer Res.Clin.Oncol.121. 317-319 (1995)
• [Publications] T.Suzuki: "Sites of labeling with N-(3-pyrene)maleimide on Ca^<2+>-transporting ATPase of the sarcoplasmic reticulum." J.Biochem.117. 881-887 (1995)
• [Publications] K.Hiramatsu: "Determination of amounts of polyamines excreted in urine:demonstration of N1,N8-diacetylspemidine and N1,N12-diacetylspermine as components commonly occurring in normal." J.Biochem.117. 107-112 (1995)
• [Publications] T.Okajima: "Cloning,sequencing,and expression in Escherichia coli cDNA encoding porcine brain UMP-CMPkinase." J.Biochem.117. 980-986 (1995)
• [Publications] T.Fukui: "Exploring the uncleotide-binding site in proteins by affinity labeling and site-directed mutagenesis" J.Biochem.117. 1139-1144 (1995)
• [Publications] Y.Kagawa: "Gene of heat shock protein of sulfur-dependent archaeal hyperthermophile Desulfurcoccus." Biochem.Biophys.Res.Commun.214. 730-736 (1995)
• [Publications] M.Sato: "ATP-hydrolyzing excitation state of the reconstituted α3β3 complex of ATP synthase from the thermphilic bacterium PS3:Structural chracteristics shown by time-resolved small-angle X-rayscattering with synchrotron radiation." J.Biochem.117. 113-119 (1995)
• [Publications] H.Endo: "A candidate gene for RNA export carrier protein has two RBDs(RNA binding domain and Ran binding domain)." Nucleic acid Symp.Series. 33. 30-32 (1995)
• [Publications] D.Takai: "Age-associated changes of mitochondrial translation and respiratory function in mouse brain." Biochem.Biophys.Res.Commun.217(印刷中). (1995)
• [Publications] S.Akiyama: "Energetics and formation of ATP synthase:Allosteric,chemiosmotic and biosynthetic control of αβ and αβγ core." Biochemistry of Cell Membrane(eds.S.Papa and J.M.Tager)Birkhauser Verlag,Basel,Swiss. 167-178 (1995)
• [Publications] H.Endo: "Heart/muscle-specific alternative splicing in ATP synthase γ-subunit pre-mRNA during muscle development." Cardiac Development and Gene Regulation(ed.Y.Tazaki),Excepta Medica. 77-97 (1995)
• [Publications] 小澤高将: "遺伝子医学:ミトコンドリア病" 第24回日本医学会会誌. 1. 207-213 (1995)
• [Publications] Y.Orii: "Facilitated intramolecular electron transfer in the Escherichia coli bo-type ubiquinol oxidase requires chloride." Biochemistry. 34. 1127-1132 (1995)
• [Publications] T.Mogi: "CuB promotes both binding and reduction of dioxgen at the heme-copper binuclear center in the Escherichia coli bo-type ubiquinol oxidase." FEBS Lett.370. 259-263 (1995)
• [Publications] M.Sato-Watanade: "Stabilization of a semiquinone redical at the high affinity quinone binding site of the Escherichia coli bo-type ubiquinol oxidase." FEBS Lett.374. 265-269 (1995)
• [Publications] S.Hirota: "Resonance Raman study on axial ligands of heme irons in cytochrome bd-type ubiquinol oxidase from Escherichia coli." Biospectroscopy. 1. 305-311 (1995)
• [Publications] M.Tsubaki: "Cyanide-binding site of bd-type ubiquinol oxidase from Escherichia coli." J.Biol.Chem.270. 28565-28569 (1995)
• [Publications] K.Denda: "Characterization of chimeric heme-copper respiratory oxidases using subunits I of Escherichia coli cytochrome bo and Halobacterium salinarium cytochrome aa_3." Biochem.Biophys.Res.Commun.217. 428-436 (1995)
• [Publications] M.Tsubaki: "FT-IR and EPR studies on cyanide-bindings to the heme-copper binuclear center of cytochrome bo-type ubiquinol oxidase from Escherichia coli:Release of CuB-cyano complex in the partially reduced states." J.Biol.Chem.271(印刷中). (1996)
• [Publications] Y.Moriyama: "Localization of N-methylmaleimide-sensitive fusion protein in pinealocytes" Neuro.Report. 6. 1757-1760 (1995)
• [Publications] Y.Tsukahara: "A subpopulation of large ganglion neurons express Isk protein mRNA:An in situ hybridization analysis in the rat eye." Mol Brain Res.29. 376-380 (1995)
• [Publications] A.Nakai: "Expression and phosphorylation of BiP/GRP78,a molecular chaperone in the endoplasmic reticulum,during the Differentiation of a mouse myeloblastic cell line." Cell Struct.Func.20. 33-39 (1995)
• [Publications] 山本章嗣: "ライソゾームの機能とV-ATPase." 生体の科学. 44. 262-266 (1995)
• [Publications] A.Miyai: "Na^+/myo-inositol cotransport is regulated by tonicity in cultured rat mesangial cells." Kidney Int.47. 473-480 (1995)
• [Publications] S.-S.Chen: "Ethanol induces heterogeneous reduction of cytochrome a-3 within perfused rat liver lobule assessed using microspectroscopy." Alcohol and Alcoholism. 30. 187-194 (1995)
• [Publications] A.Kaneko: "Activation of Na^+/H^+exchanger by hepatocyte growth factor in hepatoctes." Hepatology. 22. 629-636 (1995)
• [Publications] H.-J.Schaefer: "2,8-Diazo-ATP,a short-length bifunctional photoaffinity label for photoaffinity cross-linking of a stable F_1 in ATP synthase(from thermophilic bacteria PS3)." FEBS Lett.377. 408-412 (1995)
• [Publications] 香川靖雄: "老化のバイオサイエンス" 羊土社(印刷中), (1996)
• [Publications] 宇井理生: "生体膜-新しい膜学への招待" 講談社, 298 (1995)